Horse Chestnut Bleeding Canker Caused by Pseudomonas Syringae Pv. Aesculi in the Czech Republic

Mertelik, J.; Kloudova, K.; Pánková, Iveta; Krejzar, Václav; Kůdela, V.

doi:10.17660/actahortic.2013.990.5

Cited by 3 publications

(4 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phytophthora is a destructive parasitic fungus‐like organism causing brown rot in plants (Brasier & Strouts, ). Each tree in the present study tested negative for Phytophthora , a finding consistent with several other authors (Bultreys et al ., ; Schmidt et al ., ; de Keijzer et al ., ; Mertelik et al ., ). However, Phytophthora is identified in one or two samples of horse chestnut a year (Forest Research, ).…”

Section: Discussionmentioning

confidence: 97%

“…Since the early 2000s, the reported incidence of horse chestnut bleeding canker has dramatically increased and the disease has become severe in most areas of England, Wales and parts of Scotland. A similar increase in bleeding canker recordings has been found in the Netherlands (de Keijzer et al ., ), Belgium (Bultreys et al ., ), the Czech Republic (Mertelik et al ., ) and Germany (Schmidt et al ., ). In 2007, surveys showed 70% of horse chestnut trees in parts of England exhibited some signs of the disease, with 36% and 42% of the surveyed trees showing signs in Wales and Scotland, respectively (Forestry Commission, ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bleeding canker of horse chestnut (Aesculus hippocastanum) in Ireland: incidence, severity and characterization using DNA sequences and real‐time PCR

McEvoy¹,

O'Regan²,

Fleming

et al. 2016

Plant Pathology

View full text Add to dashboard Cite

A survey of bleeding canker disease, caused by Pseudomonas syringae pv. aesculi, was undertaken across Ireland. Incidence has become severe and can be considered epidemic, as 61% of the 1587 horse chestnut trees surveyed showed symptoms of the disease. Bacteria were isolated from a sample of trees and characterized using gyrB DNA sequencing. DNA was also extracted directly from wound tissue. The Irish P. syringae pv. aesculi genotype was identical to genotypes previously sequenced with gyrB from the UK and some other locations in Europe. Real-time PCR, using existing primers and a newly designed, more pathovar-specific primer set, was assessed for use in disease screening. With molecular screening, a total of 11 trees from a sample of 55 tested positive for P. syringae pv. aesculi in Ireland. It was more efficient to extract DNA directly from wound tissue, especially fresh bark, for disease detection than to undertake bacterial isolation with subsequent molecular analysis. A further set of sequencing primers was developed for the amplification of the gyrB gene from P. syringae pv. aesculi and their specificity was shown using a diverse sample of bacterial isolate DNAs. The study also isolated and identified other bacterial species from diseased material; some of these are known pathogens (Brenneria nigrifluens, P. marginalis and P. syringae) or have previously been identified as potentially beneficial endophytes of host trees (Erwinia billingiae, E. tolentana, P. fluorescens, P. putida and Raoultella).

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Bleeding canker of horse chestnut (Aesculus hippocastanum) in Ireland: incidence, severity and characterization using DNA sequences and real‐time PCR

McEvoy¹,

O'Regan²,

Fleming

et al. 2016

Plant Pathology

View full text Add to dashboard Cite

show abstract

“…The pathway that brought P. syringae pv. aesculi into Europe and the mechanism of its transfer to A. hippocastanum are unknown but, subsequent to its arrival, it has spread widely throughout the Netherlands and the U.K., and has been reported from Belgium, Germany, Ireland, France and the Czech Republic (Green et al ., ; EPPO, ; Mertelik et al ., ). A survey by the Forestry Commission in 2007 found that up to 40–70% of horse‐chestnuts in England, Wales and Scotland were showing symptoms of the disease (Forestry Commission, ).…”

Section: Introductionmentioning

confidence: 97%

Impact of the leaf minerCameraria ohridella(Lepidoptera:Gracillariidae) and bleeding canker disease on horse‐chestnut: direct effects and interaction

Straw

2013

Agri and Forest Entomology

View full text Add to dashboard Cite

1 The leaf miner Cameraria ohridella and bleeding canker disease (BCD) are invasive organisms causing severe damage to horse-chestnut trees in Europe. 2 Their impact and potential interaction were investigated by monitoring infestation and disease symptoms on 193 European horse-chestnuts Aesculus hippocastanum L. and 46 red horse-chestnuts Aesculus carnea J. Zeyh. over a 10-year period from 2002 to 2012. 3 Cameraria ohridella damaged up to 75% of the total leaf area of A. hippocastanum, but it had no influence on stem radial growth or general tree condition. Aesculus carnea was rarely attacked and only when growing close to heavily infested A. hippocastanum. 4 BCD, in contrast, was responsible for the death or removal of 11% of A.hippocastanum and 27% of A. carnea, and surviving trees showed a decrease in growth rate and decline in crown density. 5 Cameraria ohridella was more abundant on larger A. hippocastanum, whereas BCD was more prevalent amongst young, fast-growing A. hippocastanum, which resulted in a partial separation of the moth and disease between trees and habitats, as well as in the wider environment. 6 Trees with higher rates of leaf miner damage generally had a lower incidence of BCD and there was no evidence that C. ohridella either facilitated the spread of the disease or accentuated its impact.

show abstract

“…For example, bacterial canker of kiwifruit has rapidly spread worldwide within a short period of time [ 2 ]. Likewise, bleeding canker of horse chestnut has quickly established itself as a major threat to horse chestnut throughout Northwest Europe [ 3 ]. However, numerous diseases caused by P .…”

Section: Introductionmentioning

confidence: 99%

Extensive Field Survey, Laboratory and Greenhouse Studies Reveal Complex Nature of Pseudomonas syringae-Associated Hazelnut Decline in Central Italy

2016

View full text Add to dashboard Cite

Pseudomonas avellanae (Pav) has been reported as the causal agent of bacterial decline and bacterial canker of hazelnut in Italy and Greece, respectively. Both hazelnut diseases were reported to be similar in terms of symptoms, severity and persistence. In this study, we found that both symptomatic and asymptomatic trees in the field were colonized by Pav. Multilocus Sequence Typing (MLST) analysis showed that Pav strains isolated during this study in Italy belong to the P. syringae phylogroup 1 and they are closely related to Pav strains previously isolated in Greece from hazelnut bacterial canker. On the other hand, strains isolated in earlier studies from hazelnut decline in Italy belong to both phylogroup 1 and 2 of P. syringae. Both phylogroup 1 strains of P. syringae from Greece and Italy are different than strains isolated in this study in terms of their capacity to excrete fluorescent pigments on different media. Despite the same plant genotype and cropping practices adopted, the incidence of hazelnut decline ranged from nearly 0 to 91% across our study sites. No disease developed on plants inoculated with Pav through wounding while leaf scar inoculations produced only mild disease symptoms. Based on our results and the previously reported correlation between pedo-climatic conditions and hazelnut decline, we conclude that hazelnut decline in central Italy could be incited by a combination of predisposing (adverse pedo-climatic conditions) and contributing factors (Pav). Because this is a true decline different from “bacterial canker” described in Greece, we refer to it as hazelnut decline (HD).

show abstract

Horse Chestnut Bleeding Canker Caused by Pseudomonas Syringae Pv. Aesculi in the Czech Republic

Cited by 3 publications

References 4 publications

Bleeding canker of horse chestnut (Aesculus hippocastanum) in Ireland: incidence, severity and characterization using DNA sequences and real‐time PCR

Bleeding canker of horse chestnut (Aesculus hippocastanum) in Ireland: incidence, severity and characterization using DNA sequences and real‐time PCR

Impact of the leaf minerCameraria ohridella(Lepidoptera:Gracillariidae) and bleeding canker disease on horse‐chestnut: direct effects and interaction

Extensive Field Survey, Laboratory and Greenhouse Studies Reveal Complex Nature of Pseudomonas syringae-Associated Hazelnut Decline in Central Italy

Contact Info

Product

Resources

About