Horseradish peroxidase-functionalized gold nanoparticle label for amplified immunoanalysis based on gold nanoparticles/carbon nanotubes hybrids modified biosensor

Cui, Rongjing; Huang, Haiping; Yin, Zheng‐Zhi; Gao, Di; Zhu, Jun‐Jie

doi:10.1016/j.bios.2008.01.034

Cited by 213 publications

(51 citation statements)

References 26 publications

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“…Nanohybrids of GNPs and CNTs offer a more effective immobilization matrix than that of GNPs alone. Cui et al described a sensitive electrochemical biosensor with GNPs/ CNTs hybrids platform modified glass carbon electrode [101]. The CNTs were first activated and assembled on the electrode surface.…”

Section: Gnps Act As Immobilization Platformmentioning

confidence: 99%

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Gold nanoparticle-based biosensors

2010

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The unique properties of gold nanoparticles have stimulated the increasing interest in the application of GNPs in interfacing biological recognition events with signal transduction and in designing biosensing devices exhibiting novel functions. The optical properties of GNPs provide wide range opportunities for construction optical biosensors. The excellent biocompatibility, conductivity, catalytic properties and high surface-to-volume ratio and high density of GNPs facilitate extensive application of GNPs in construction of electrochemical and piezoelectric biosensors with enhanced analytical performance with respect to other biosensor designs. In this article, the recent advances in construction of GNP-based optical, electrochemical and piezoelectric biosensors are reviewed, and some illustrative examples given, with a focus on the roles GNPs play in the biosensing process and the mechanism of GNPs for improving the analytical performances. Finally, the review concludes with an outline of present and future research for the real-world applications.

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Section: Gnps Act As Immobilization Platformmentioning

confidence: 99%

“…Subsequently the GNPs were electrostatically adsorbed to the CNTs surface, followed by immobilization of the biosensing element, goat anti-human IgG, onto the GNPs. This approach exhibited a linear response range to IgG between 0.125 and 80 ng/mL with a detection limit of 40 pg/mL [101].…”

Section: Gnps Act As Immobilization Platformmentioning

confidence: 99%

Gold nanoparticle-based biosensors

2010

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“…Enhanced sensitivity was achieved by loading a large amount of enzyme towards an individual sandwich immunological reaction event. Recently, various types of nanomaterials have been used as carriers for loading enzymes and antibodies to enhance sensitivity, including gold nanoparticles, 24 irregularly shaped gold nanoparticles, 25 nanosilica particles, 26 carbon nanoparticles, 27,28 carbon nanotubes, 29 and graphene oxide. 30 For example, Chen et al have proposed a highly sensitive electrochemical immunosensor using irregular gold nanoparticles as carriers of horseradish peroxidase (HRP)-anti-AFP for signal amplification.…”

Section: Introductionmentioning

confidence: 99%

Fe3O4/Au magnetic nanoparticle amplification strategies for ultrasensitive electrochemical immunoassay of alfa-fetoprotein

Gan¹,

Jin²,

Li³

et al. 2011

IJN

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Background The purpose of this study was to devise a novel electrochemical immunosensor for ultrasensitive detection of alfa-fetoprotein based on Fe 3 O 4 /Au nanoparticles as a carrier using a multienzyme amplification strategy. Methods and results Greatly enhanced sensitivity was achieved using bioconjugates containing horseradish peroxidase (HRP) and a secondary antibody (Ab 2 ) linked to Fe 3 O 4 /Au nanoparticles (Fe 3 O 4 /Au-HRP-Ab 2 ) at a high HRP/Ab 2 ratio. After a sandwich immunoreaction, the Fe 3 O 4 /Au-HRP-Ab 2 captured on the electrode surface produced an amplified electrocatalytic response by reduction of enzymatically oxidized hydroquinone in the presence of hydrogen peroxide. The high content of HRP in the Fe 3 O 4 /Au-HRP-Ab 2 could greatly amplify the electrochemical signal. Under optimal conditions, the reduction current increased with increasing alfa-fetoprotein concentration in the sample, and exhibited a dynamic range of 0.005–10 ng/mL with a detection limit of 3 pg/mL. Conclusion The amplified immunoassay developed in this work shows good precision, acceptable stability, and reproducibility, and can be used for detection of alfa-fetoprotein in real samples, so provides a potential alternative tool for detection of protein in the laboratory. Furthermore, this immunosensor could be regenerated by simply using an external magnetic field.

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“…20,21 Our group has reported extensively on LbL based single and bi-enzyme biosensing systems incorporating multi-walled carbon nanotubes (MWCNTs) immobilized with organophosphorus hydrolase (OPH) and acetylcholinesterase (AChE), for discriminative detection between organophosphorus and non-organophosphorus pesticides. 13,22 In these organized LbL nanostructures, a homogenous and stable CNT-based assembly of multi-enzyme interfaces with desired architecture provides control over the position of the polyelectrolyte and the enzyme molecules compared to random hydrogels.…”

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confidence: 99%

Layer-by-Layer Assembly of Carbon Nanotubes Modified with Invertase/Glucose Dehydrogenase Cascade for Sucrose/O₂Biofuel Cell

Zhang

Arugula

Williams

et al. 2016

J. Electrochem. Soc.

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A layer-by-layer (LbL) assembly technique was employed to modify glassy carbon electrodes (GCEs) and screen printed electrodes (SPEs) utilizing multiwalled carbon nanotubes (MWCNTs)/polyelectrolyte binary composites and an enzyme cascade to facilitate efficient electron transfer in sucrose/O 2 biofuel cell. In this study, MWCNTs immobilized invertase (INV) and glucose dehydrogenase (GDH) were alternatively assembled upon polyethyleneimine (PEI) and DNA nanocomposites to construct a bioanode.[Ni(phendion)(phen)]Cl 2 complex and methylene green (MG) were investigated as redox mediators for electrocatalytic oxidation of NADH at reduced overpotentials. The LbL architecture showed advantages for sequential enzymatic reaction that favored the efficient penetration of substrate and products in a cascade system while MWCNTs facilitated the efficient electron transfer from sucrose oxidation and enhancement of the current density. With a GCE bioanode modified with a MG or Ni complex, the biofuel cell produced a higher power density (μW/cm 2 ) with 145.8% and 130.11% enhancement comparing to a SPE bioanode, respectively. Moreover, the Ni complex modified GCEs and SPEs produced power densities (μW/cm 2 ) 93.7% and 107.0% higher compared to MG modified bioanodes, respectively. The maximum current density of 1400 ± 46 μA/cm 2 was obtained with the Ni complex on GCE at an OCP of 604 ± 17 mV with a maximum power density of 405 ± 6 μW/cm 2 . The LbL assembly showed great feasibility as a simple and efficient way to construct controlled MWCNT-multi-enzyme modified electrodes for biofuel cell applications. Biofuel cells convert the chemical energy of biofuels into electric energy via the enzymatically catalyzed oxidation and reduction reactions. One of the most important factors affecting the performance of fuel cell is the fabrication of bioanode which has great influence in generation of electrical power outputs. Therefore developing effective bioanodes remains critical. Till date, several reports have been published on various approaches to assemble enzymes on the anodes in BFCs utilizing carbon based materials.1-4 Amongst those layerby-layer (LbL) assembly technique via electrostatic interactions of oppositely charged species has emerged as a very attractive way to construct multilayer films of polyelectrolytes, biomolecules, 5 and organic materials 6-8 offering advantages in various fields, such as surface functionalization, 9,10 drug delivery, 11,12 and biosensing. [13][14][15] Its application was considered plausible in the development of amperometric biosensors initiating vast research activities on biosensors comprised of LbL organized multilayers. LbL nanostructures decorated with multi-enzymes were proven to be one of the successful strategies to establish high electrical performance, long-term stability and long lifetime in bioelectronics devices.14,16-18 For example, LbL structures consisting of Au nanoparticles (AuNPs), thiol-functionalized polyaniline and glucose oxidase (GOx) were fabricated for glucose biosensing ...

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Horseradish peroxidase-functionalized gold nanoparticle label for amplified immunoanalysis based on gold nanoparticles/carbon nanotubes hybrids modified biosensor

Cited by 213 publications

References 26 publications

Gold nanoparticle-based biosensors

Gold nanoparticle-based biosensors

Fe3O4/Au magnetic nanoparticle amplification strategies for ultrasensitive electrochemical immunoassay of alfa-fetoprotein

Layer-by-Layer Assembly of Carbon Nanotubes Modified with Invertase/Glucose Dehydrogenase Cascade for Sucrose/O₂Biofuel Cell

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Horseradish peroxidase-functionalized gold nanoparticle label for amplified immunoanalysis based on gold nanoparticles/carbon nanotubes hybrids modified biosensor

Cited by 213 publications

References 26 publications

Gold nanoparticle-based biosensors

Gold nanoparticle-based biosensors

Fe3O4/Au magnetic nanoparticle amplification strategies for ultrasensitive electrochemical immunoassay of alfa-fetoprotein

Layer-by-Layer Assembly of Carbon Nanotubes Modified with Invertase/Glucose Dehydrogenase Cascade for Sucrose/O2Biofuel Cell

Contact Info

Product

Resources

About

Layer-by-Layer Assembly of Carbon Nanotubes Modified with Invertase/Glucose Dehydrogenase Cascade for Sucrose/O₂Biofuel Cell