Hospital-acquired adenovirus 7h infantile respiratory infection in Chile

Palomino, María Angélica; Larrañaga, Carmen; Avendaño, Luis F.

doi:10.1097/00006454-200006000-00007

Cited by 37 publications

(42 citation statements)

References 9 publications

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“…Respiratory syncytial virus (RSV) is the principal cause of ALRI, causing yearly winter epidemics that frequently challenge health resources (3,4,5,9,12,15,21). The expanded use of new techniques has facilitated the local identification of etiologic agents, allowing the comparison of clinical and epidemiological features with biological agent characteristics (2,6,7,14,16,17,19). The aim of this study is to present an overview of RSV infection causing pediatric admissions for ALRI in Chile over a long enough period of time to support recommendations for better clinical and epidemiological management.…”

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confidence: 99%

“…Confirmatory IFA for RSV, adenovirus, influenza A and B viruses, and parainfluenza viruses 1 to 3 were performed for cultures with and without CPE. Standard IFA was done using monoclonal antibodies provided by L. Anderson, Centers for Disease Control and Prevention (CDC), Atlanta, Ga., and P. Pothier, Dijon, France, as previously described (3,17). From 1994 to 2000, RSV strains from NPA or positive cultures were grouped by IFA with monoclonal antibodies 133-1H and 93-11C (CDC) and 2B8 (P. Pothier) for group A typing and 102-10B (CDC) for group B typing (1).…”

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confidence: 99%

“…The surveillance policy of collecting 25 to 50 samples per month from inpatients with ALRI is as efficient as taking a greater number of outpatient samples. IFA is sufficiently sensitive and easy to implement for routine RSV detection (3,17).…”

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confidence: 99%

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Surveillance for Respiratory Syncytial Virus in Infants Hospitalized for Acute Lower Respiratory Infection in Chile (1989 to 2000)

Avendaño¹,

Palomino

Larrañaga³

2003

J Clin Microbiol

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Hospitalized infants (4,618) were studied for lower respiratory infections from 1989 through 2000 by routine immunofluorescence assay and viral isolation. The hospitalization rate for respiratory syncytial virus (RSV) averaged 2% per year. The fatality rate was 0.1%. Monthly RSV detection varied from 14 to 88%, and epidemics lasted 3.5 to 6 months. From 1994 high-early versus low-late epidemic patterns alternately were observed, the first influenced by a group B strain.Acute respiratory infections are a major worldwide health problem because of associated high morbidity and mortality rates. In Chile acute lower respiratory infections (ALRI) are the primary cause of hospital admissions during infancy. Respiratory syncytial virus (RSV) is the principal cause of ALRI, causing yearly winter epidemics that frequently challenge health resources (3,4,5,9,12,15,21). The expanded use of new techniques has facilitated the local identification of etiologic agents, allowing the comparison of clinical and epidemiological features with biological agent characteristics (2,6,7,14,16,17,19). The aim of this study is to present an overview of RSV infection causing pediatric admissions for ALRI in Chile over a long enough period of time to support recommendations for better clinical and epidemiological management. We present 12 years of data from immunofluorescence assays and isolation for routine viral diagnosis in the pediatric context.A total of 4,618 out of 21,912 children under 2 years of age admitted for ALRI to the Roberto del Rio Children's Hospital, Santiago, Chile, were studied prospectively for RSV from January 1989 to December 2000. Exclusion criteria were prematurity; recurrent wheezing or asthma; and underlying chronic pulmonary, cardiac, or neurological diseases. During the cold season (April to September) two ALRI admissions were randomly selected per day in order to collect 40 to 60 cases per month. During the warm season (October to March) almost all patients were enrolled. For each patient, clinical features, personal and family history of asthma, and the admission diagnosis were obtained from hospital records.Nasopharyngeal aspirates (NPA) were routinely obtained within the first 48 h after admission and were immediately transported to the laboratory for viral isolation and indirect immunofluorescence assay (IFA). Weekday specimens were processed on the same day, but those obtained on weekends were kept at 4°C until processing. Samples were inoculated into HEp-2 and MDCK cells and observed for cytopathic effect (CPE) for 1 week. Confirmatory IFA for RSV, adenovirus, influenza A and B viruses, and parainfluenza viruses 1 to 3 were performed for cultures with and without CPE. Standard IFA was done using monoclonal antibodies provided by L. Anderson, Centers for Disease Control and Prevention (CDC), Atlanta, Ga., and P. Pothier, Dijon, France, as previously described (3, 17). From 1994 to 2000, RSV strains from NPA or positive cultures were grouped by IFA with monoclonal antibodies 133-1H and 93-11C (CDC) and 2B8 ...

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Surveillance for Respiratory Syncytial Virus in Infants Hospitalized for Acute Lower Respiratory Infection in Chile (1989 to 2000)

Avendaño¹,

Palomino

Larrañaga³

2003

J Clin Microbiol

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“…Hospitalised patients presenting haematological diseases, with prolonged hospital stays, are at a high risk of severe HAdV infection and represent a potential source of nosocomial outbreaks 25 . Studies have reported rates of HAdV infection ranging from 2 to 21%, with a mortality rate of 10-60%, depending on the immunosuppressive regimen used 26 .…”

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confidence: 99%

Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection

Watanabe

Carraro²,

Camargo

et al. 2013

Rev. Soc. Bras. Med. Trop.

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Introduction: Human adenoviruses (HAdV) play an important role in the aetiology of severe acute lower respiratory infection, especially in immunocompromised individuals. The aim of the present study was to detect HAdV using two different methods, direct fluorescence assay (DFA) and nested polymerase chain reaction (nested PCR), in samples collected from patients with acute respiratory infection (ARI) within 7 days of symptom onset. Methods: Samples (n=643) were collected from patients in different risk groups from 2001 to 2010: 139 adult emergency room patients (ERP); 205 health care workers (HCW); 69 renal transplant outpatients (RTO); and 230 patients in a haematopoietic stem cell transplantation program (HSCT). Results: Adenovirus was detected in 13.2% of the 643 patients tested by DFA and/or PCR: 6/139 (4.3%) adults in the ERP group, 7/205 (3.4%) in the HCW group, 4/69 (5.8%) in the RTO group and 68/230 (29.5%) in the HSCT patient group. Nested PCR had a higher detection rate (10%) compared with the DFA test (3.8%) (p<0.001). HSCT patients exhibited a significantly higher rate of HAdV infection. Conclusions: The adenovirus detection rate of the nested PCR assay was higher than that of the DFA test. However, the use of molecular methods in routine diagnostic laboratory work should be evaluated based on the specific circumstances of individual health services.

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“…48 En este sentido son factores de riesgo: edad menor de 12 meses, antecedente de hospitalización reciente por infección del tracto respiratorio inferior, inmunodeficiencia subyacente, antecedentes de neumonía o asma e infección nosocomial por adenovirus. 49,50 En países endémi-cos también destacan las micobacterias (M. tuber-culosis y micobacterias atípicas). 29 Es importante tener presente la bronquitis bacteriana persistente como causa de bronquiectasias.…”

Section: Inmunodeficienciasunclassified

Bronquiectasias en pediatría, aproximación diagnóstica y tratamiento

et al. 2012

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RESUMENLas bronquiectasias son un problema de salud en países desarrollados y en vías de desarrollo. La fibrosis quística es una causa importante, si bien fuera de ésta existen causas que convierten a las bronquiectasias no relacionadas con fibrosis quística en un diagnóstico frecuente. Su diagnóstico precoz basado en la clínica y posterior confirmación radiológica es el punto de partida para determinar la etiología e instaurar un tratamiento dirigido a la causa subyacente. Para ello es menester una evaluación ordenada y sistemá-tica. Se presenta una revisión sobre la etiología y tratamiento de las bronquiectasias, especialmente de las no relacionadas con fibrosis quística. Palabras clave: bronquiectasias, fibrosis quística, antibióticos, infección bronquial, diagnóstico. SUMMARYBronchiectasis is still an important health problem in both, developed and developing countries. Cystic fibrosis is one of the most important causes, but a great variety of other causes makes non cystic fibrosis bronchiectasis a relatively frequent diagnosis. Early diagnosis based on medical history and radiological confirmation is only the starting point to determine the specific etiology, in order to establish a treatment focused on the underlying cause. For this purpose, an orderly and systematic diagnostic evaluation is required. A review article about bronchiectasis, particularly those not related to cystic fibrosis, is presented.

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Hospital-acquired adenovirus 7h infantile respiratory infection in Chile

Abstract: The high secondary attack rate observed, stresses the importance of adequate isolation of patients and the need for rapid and sensitive viral diagnosis.

Cited by 37 publications

References 9 publications

Surveillance for Respiratory Syncytial Virus in Infants Hospitalized for Acute Lower Respiratory Infection in Chile (1989 to 2000)

Surveillance for Respiratory Syncytial Virus in Infants Hospitalized for Acute Lower Respiratory Infection in Chile (1989 to 2000)

Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection

Bronquiectasias en pediatría, aproximación diagnóstica y tratamiento

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