Host and viral RNA-binding proteins involved in membrane targeting, replication and intercellular movement of plant RNA virus genomes

Abstract

Many plant viruses have positive-strand RNA [(+)RNA] as their genome. Therefore, it is not surprising that RNA-binding proteins (RBPs) play important roles during (+)RNA virus infection in host plants. Increasing evidence demonstrates that viral and host RBPs play critical roles in multiple steps of the viral life cycle, including translation and replication of viral genomic RNAs, and their intra- and intercellular movement. Although studies focusing on the RNA-binding activities of viral and host proteins, and their associations with membrane targeting, and intercellular movement of viral genomes have been limited to a few viruses, these studies have provided important insights into the molecular mechanisms underlying the replication and movement of viral genomic RNAs. In this review, we briefly overview the currently defined roles of viral and host RBPs whose RNA-binding activity have been confirmed experimentally in association with their membrane targeting, and intercellular movement of plant RNA virus genomes.

Zinc and Copper Ions Differentially Regulate Prion-Like Phase Separation Dynamics of Pan-Virus Nucleocapsid Biomolecular Condensates

“…Intriguing research suggests that highly disordered RNA chaperones were among the earliest proteins to evolve, with their PrLDs providing solubilization and entropic exclusion effects and the bypassing of energy consuming iterative annealing activities [ 119 , 120 ]. Plant virus movement proteins (MPs) are also disordered and possess a wide range of functions, such as interacting with viral proteins and vRNA to form ribonucleoprotein complexes facilitating cell-to-cell and long-distance movement of the viral genome within the plant body [ 121 , 122 ]. The cysteine-histidine-rich region of cucumber mosaic virus MP contributes to plasmodesmal targeting and Zn 2+ binding and pathogenesis.…”

In-depth proteomic analysis of Varroa destructor: Detection of DWV-complex, ABPV, VdMLV and honeybee proteins in the mite

“…On contrary, the Q substitution had significantly higher score for DNA-dependent protein kinase compared to the K and A substitution. Both phosphoglycerate kinase 72 73 and DNA-dependent protein kinase 74 75 were found associated with virus replication. Therefore, we suggest that the A/K/Q single-amino-acid substitution influence DWV replication in honeybee.…”

Harnessing host ROS-generating machinery for the robust genome replication of a plant RNA virus

“…Plant RNA viruses must interact and use host factors at every step of the replication cycle (e.g., RNA synthesis, virus assembly, movement, counterdefense, and transmission). Examples include eukaryotic translation initiation factors, RNA-binding proteins, membrane trafficking machinery, and lipid-generating enzymes (17,19,20,55,56). These host factors simultaneously serve as virus restriction determinants if they have mutations that are unfavorable for virus replication (56).…”