In-depth proteomic analysis of Varroa destructor: Detection of DWV-complex, ABPV, VdMLV and honeybee proteins in the mite

Erban, Tomáš; Harant, Karel; Hubálek, Martin; Vítámvás, Pavel; Kamler, Martin; Poltronieri, Palmiro; Tyl, Jan; Markovic, Martin; Titěra, Dalibor

doi:10.1038/srep13907

Cited by 42 publications

(42 citation statements)

References 90 publications

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“…The host in which predominant amplification occurs remains unclear. A recent study demonstrated the absence of non-structural and high abundance of structural proteins in Varroa, suggesting that DWV proteins accumulated in the gut after feeding and not as a result of viral replication in the mite [44]. However, studies using FISH (Fluorescence in situ hybridization) probes or immunohistochemical techniques may help resolve this uncertainty.…”

Section: Discussionmentioning

confidence: 99%

A Comparison of Deformed Wing Virus in Deformed and Asymptomatic Honey Bees

Brettell

Mordecai

Schroeder

et al. 2017

Insects

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Deformed wing virus (DWV) in association with Varroa destructor is currently attributed to being responsible for colony collapse in the western honey bee (Apis mellifera). The appearance of deformed individuals within an infested colony has long been associated with colony losses. However, it is unknown why only a fraction of DWV positive bees develop deformed wings. This study concerns two small studies comparing deformed and non-deformed bees. In Brazil, asymptomatic bees (no wing deformity) that had been parasitised by Varroa as pupae had higher DWV loads than non-parasitised bees. However, we found no greater bilateral asymmetry in wing morphology due to DWV titres or parasitisation. As expected, using RT-qPCR, deformed bees were found to contain the highest viral loads. In a separate study, next generation sequencing (NGS) was applied to compare the entire DWV genomes from paired symptomatic and asymptomatic bees from three colonies on two different Hawaiian islands. This revealed no consistent differences between DWV genomes from deformed or asymptomatic bees, with the greatest variation seen between locations, not phenotypes. All samples, except one, were dominated by DWV type A. This small-scale study suggests that there is no unique genetic variant associated with wing deformity; but that many DWV variants have the potential to cause deformity.

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Section: Discussionmentioning

confidence: 99%

A Comparison of Deformed Wing Virus in Deformed and Asymptomatic Honey Bees

Brettell

Mordecai

Schroeder

et al. 2017

Insects

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“…Instead, most mite-parasitized pupae still develop into bees which emerge without showing obvious symptoms, hence, the parasitizing mites just either act as mechanical vectors of DWV or are even DWV-free (Yue and Genersch, 2005). Given that in a non-collapsing colony the majority of mites will not harbour replicating DWV, it is not surprising that no evidence for DWV replication in mites was found when only four mites were analyzed (Santillán-Galicia et al, 2008) or when phoretic mites collected from healthy adult bees were used for analysis (Erban et al, 2015). These results were correct for the analyzed mites, but the studies suffered from not having analyzed the right mites.…”

Section: Does Dwv Replicate In Mites?mentioning

confidence: 99%

In vivo evolution of viral virulence: switching of deformed wing virus between hosts results in virulence changes and sequence shifts

Gisder

Möckel

Eisenhardt

et al. 2018

Environmental Microbiology

149

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Summary The health of the Western honey bee is threatened by a global epidemic of deformed wing virus (DWV) infections driven by the ectoparasitic mite Varroa destructor acting as mechanical and biological virus vector. Three different variants of DWV, DWV‐A, ‐B and ‐C exist. Virulence differences between these variants and their relation to V. destructor are still controversially discussed. We performed laboratory experiments to analyze the virulence of DWV directly isolated from crippled bees (DWVP0) or after one additional passage in bee pupae (DWVP1). We demonstrated that DWVP0 was more virulent than DWVP1 for pupae, when pupal mortality was taken as virulence marker, and for adult bees, when neurotropism and cognitive impairment were taken as virulence markers. Phylogenetic analysis supported that DWV exists as quasispecies and showed that DWVP0 clustered with DWV‐B and DWVP1 with DWV‐A when the phylogeny was based on the master sequences of the RNA‐dependent RNA polymerase but not so when it was based on the VP3 region master sequences. We propose that switching of DWV between the bee and the mite host is accompanied by changes in viral sequence, tissue tropism and virulence and that the RNA‐dependent RNA polymerase is involved in determining host range and virulence.

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“…The impact of varroa mites on honey bee health is particularly severe because varroa can also act as a vector for a number of bee viruses [145,146,147,148]. The act of feeding on bees by varroa directly injects a large number of viral particles into the host [145,147,149], selecting for certain (more virulent) virus strains and causing viral pathologies, such as immunosuppression, weight loss, decreased flight ability, and reduced lifespan [19,150,151,152].…”

Section: Parasites and Queen Qualitymentioning

confidence: 99%

Queen Quality and the Impact of Honey Bee Diseases on Queen Health: Potential for Interactions between Two Major Threats to Colony Health

Amiri

Strand

Rueppell

et al. 2017

Insects

130

103

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Western honey bees, Apis mellifera, live in highly eusocial colonies that are each typically headed by a single queen. The queen is the sole reproductive female in a healthy colony, and because long-term colony survival depends on her ability to produce a large number of offspring, queen health is essential for colony success. Honey bees have recently been experiencing considerable declines in colony health. Among a number of biotic and abiotic factors known to impact colony health, disease and queen failure are repeatedly reported as important factors underlying colony losses. Surprisingly, there are relatively few studies on the relationship and interaction between honey bee diseases and queen quality. It is critical to understand the negative impacts of pests and pathogens on queen health, how queen problems might enable disease, and how both factors influence colony health. Here, we review the current literature on queen reproductive potential and the impacts of honey bee parasites and pathogens on queens. We conclude by highlighting gaps in our knowledge on the combination of disease and queen failure to provide a perspective and prioritize further research to mitigate disease, improve queen quality, and ensure colony health.

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In-depth proteomic analysis of Varroa destructor: Detection of DWV-complex, ABPV, VdMLV and honeybee proteins in the mite

Cited by 42 publications

References 90 publications

A Comparison of Deformed Wing Virus in Deformed and Asymptomatic Honey Bees

A Comparison of Deformed Wing Virus in Deformed and Asymptomatic Honey Bees

In vivo evolution of viral virulence: switching of deformed wing virus between hosts results in virulence changes and sequence shifts

Queen Quality and the Impact of Honey Bee Diseases on Queen Health: Potential for Interactions between Two Major Threats to Colony Health

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