Host Cell-Trichomonad Interactions and Virulence Assays Using in In Vitro Systems

Honigberg, B. M.

doi:10.1007/978-1-4612-3224-7_9

Cited by 17 publications

(14 citation statements)

References 49 publications

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“…It has been suggested that natural antibodies to the parasite, generated by cross-reacting antigens with the normal vaginal flora, may enhance the complement-mediated lysis (Shaio et al 1991). Specific antibody responses to T. vaginalis antigens in serum have also been reported (Alderete et al 1991c); however, similar to their local counterparts, these circulating antibody levels also differ and appear to have no function in helping the host to get rid of the infection (Honigberg 1990). Although an association between the presence of local antibody and low parasite counts has been postulated, there is no conclusive evidence to suggest that the presence of IgA antibodies is specifically related to the immune response to T. vaginalis.…”

Section: Discussionmentioning

confidence: 93%

Specific IgA response, T-cell subtype and cytokine profile in experimental intravaginal trichomoniasis

Kaur

Gupta

et al. 2001

Parasitol Res

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Trichomoniasis caused by Trichomonas vaginalis may lead to either a complete absence of symptoms or to severe inflammatory manifestations in infected women. Studies of the role of immune responses in the pathogenesis and varied symptomatology of this disease are lacking. Mice may prove useful as an experimental model for intravaginal trichomoniasis in developing an understanding of the role of local immune responses in the pathogenesis and varied symptomatology of this disease. The present study reports the levels of anti-Trichomonas IgA antibodies in serum and vaginal washes, and T-cell subtype and cytokine profile in vaginal cervical tissues of mice infected intravaginally with T. vaginalis isolates from 15 symptomatic and 15 asymptomatic women. It also correlates the responses with symptomatology of the patients. Successful intravaginal infection was established by inoculating T. vaginalis in BALB/c mice preinoculated with Lactobacillus acidophilus and pretreated with oestradiol. A significant increase in specific IgA antibody levels was detected with enzyme-linked immunosorbent assay in vaginal secretions and serum samples collected on the 7th post-infection day from animals infected with isolates from asymptomatic women when compared with mice infected with isolates from symptomatic women. T-cell subset analysis showed significant differences, with increased CD4+ T-cell count in animals infected with isolates from asymptomatic women compared with animals infected using isolates from symptomatic women. No difference in CD8+ T cells was observed between the two groups. Cytokine profile revealed significantly higher (P < 0.001) production of gamma-IFN and IL-2 in mice infected with asymptomatic isolates compared with animals infected with symptomatic isolates, using T. vaginalis crude antigen extract and nonspecific mitogen (ConA) as stimulants for vaginal cervical lymphocytes. However, no difference in IL-4 levels was observed in the two groups of animals. In contrast, significant increase in tumour necrosis factor (TNF-alpha) levels was observed in animals infected with asymptomatic isolates compared with those infected with isolates from symptomatic women and controls, thereby indicating that TNF-alpha may play an important role in the inflammatory response to trichomoniasis. The study further suggests that specific IgA antibodies might help to protect asymptomatic individuals from severe infection and T-lymphocytes may play an important function in the eradication of the parasite. The cytokine profile indicated the involvement of Th-1 like responses in mice infected with asymptomatic isolates, compared with those infected with symptomatic isolates.

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Section: Discussionmentioning

confidence: 93%

Specific IgA response, T-cell subtype and cytokine profile in experimental intravaginal trichomoniasis

Kaur

Gupta

et al. 2001

Parasitol Res

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“…Others proved that cytoadherence to vaginal epithelium was higher in isolates from SYM women than ASYM one [34]. T. vaginalis isolated from symptomatic women was able to stimulate strong chemotactic response towards polymorphonuclear leukocytes (PMN) as compared to those isolated from asymptomatic one [35]. In experimental study, a higher inflammatory response also observed in mice infected with symptomatic isolates as compared to those infected with asymptomatic isolates [36].…”

Section: Discussionmentioning

confidence: 95%

Kinetics of Serum and Local Leukotriene B<sub>4</sub> Response in Experimental Intravaginal Trichomoniasis by <i>T. vaginalis</i> Isolates from Symptomatic and Asymptomatic Women

Eida¹,

Eida²,

Salem³

2015

AID

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“…The adhesions are concentrated on the side opposite the undulating membrane while laminin binding proteins are ubiquitous on the entire surface of the parasite (Costa et al, 1988). Parasite binds to numerous host macromolecules which may serve a nutritive purpose while few may protect the parasite by modifying their host defences, thereby helping in evasion of the host immunity (Honigberg, 1990). In Vitro studies have indicated time dependent significant difference in the percentage of vaginal epithelial cells (VEC) attached by trichomonads as well as number of parasites attached per VEC in T.vaginalis isolates from symptomatic and asymptomatic women.…”

Section: Adherence and Adhesionsmentioning

confidence: 99%

“…The differences in virulence in different strains by In-Vivo (Honigberg et al, 1966) and In-Vitro (Honigberg, 1990) assays have been reported. The In-Vivo virulence assays in experimental mouse model have shown that intraperitoneal infection of trichomonads produces visceral (pancreatic and hepatic) necrosis and extent of necrosis is proportional to the level of virulence of the inoculated strain, which can result in death.…”

Section: Strain Variationmentioning

confidence: 99%

Human Trichomoniasis due to Trichomonas vaginalis - Current Perspectives

Malla¹

2012

Sexually Transmitted Infections

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Host Cell-Trichomonad Interactions and Virulence Assays Using in In Vitro Systems

Cited by 17 publications

References 49 publications

Specific IgA response, T-cell subtype and cytokine profile in experimental intravaginal trichomoniasis

Specific IgA response, T-cell subtype and cytokine profile in experimental intravaginal trichomoniasis

Kinetics of Serum and Local Leukotriene B<sub>4</sub> Response in Experimental Intravaginal Trichomoniasis by <i>T. vaginalis</i> Isolates from Symptomatic and Asymptomatic Women

Human Trichomoniasis due to Trichomonas vaginalis - Current Perspectives

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Host Cell-Trichomonad Interactions and Virulence Assays Using in In Vitro Systems

Cited by 17 publications

References 49 publications

Specific IgA response, T-cell subtype and cytokine profile in experimental intravaginal trichomoniasis

Specific IgA response, T-cell subtype and cytokine profile in experimental intravaginal trichomoniasis

Kinetics of Serum and Local Leukotriene B&lt;sub&gt;4&lt;/sub&gt; Response in Experimental Intravaginal Trichomoniasis by &lt;i&gt;T. vaginalis&lt;/i&gt; Isolates from Symptomatic and Asymptomatic Women

Human Trichomoniasis due to Trichomonas vaginalis - Current Perspectives

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Kinetics of Serum and Local Leukotriene B<sub>4</sub> Response in Experimental Intravaginal Trichomoniasis by <i>T. vaginalis</i> Isolates from Symptomatic and Asymptomatic Women