Kinetics of Serum and Local Leukotriene B&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt; Response in Experimental Intravaginal Trichomoniasis by &amp;lt;i&amp;gt;T. vaginalis&amp;lt;/i&amp;gt; Isolates from Symptomatic and Asymptomatic Women

Eida, Amany M; Eida, Omima M; Salem, Ahmed Saied

doi:10.4236/aid.2015.51004

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…These data therefore suggest that LTs are dispensable to VVC immunopathology. Based on the detectable and increased presence of vaginal LTB 4 during acute VVC shown here, as well as during experimental vaginal Trichomonas vaginalis infection ( Eida et al, 2015 ), it will be interesting to determine the mechanisms of LT production and receptor expression, both locally and systemically, during vaginal infection. Unfortunately, the assessment of LTs (locally or systemically) in the presence of the drugs is not informative since the drugs act as receptor antagonists and not synthesis inhibitors.…”

Section: Discussionmentioning

confidence: 94%

Leukotrienes Are Dispensable for Vaginal Neutrophil Recruitment as Part of the Immunopathological Response During Experimental Vulvovaginal Candidiasis

et al. 2021

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Recruitment of polymorphonuclear neutrophils (PMNs) into the vaginal lumen is the hallmark of an acute immunopathologic inflammatory response during vulvovaginal candidiasis (VVC) caused by Candida albicans. Recurrent VVC (RVVC) remains a chronic health burden in affected women worldwide despite the use of antifungal therapy. Based on the role leukotrienes (LTs) play in promoting inflammation, leukotriene receptor antagonists (LTRAs) targeted for LTB4 (etalocib) or LTC4, LTD4, and LTE4 (zafirlukast or montelukast) have been shown to reduce inflammation of epithelial tissues. An open-label pilot study using long-term regimens of zafirlukast in women with RVVC indicated the potential for some relief from recurrent episodes. To investigate this clinical observation further, we evaluated the effects of LT antagonistic agents and LT deficiency on the immunopathogenic response in a mouse model of VVC. Results showed that mice given daily intraperitoneal injections of individual LTRAs, starting 2days prior to vaginal inoculation with C. albicans and continuing through 14days post-inoculation, had no measurable reduction in PMN migration. The LTRAs were also ineffective in reducing levels of the hallmark vaginal inflammatory markers (S100A8, IL-1β) and tissue damage (LDH) associated with the immunopathogenic response. Finally, LT-deficient 5-lipoxygenase knockout mice showed comparable levels of vaginal fungal burden and PMN infiltration to wild-type mice following inoculation with a vaginal (ATCC 96113) or laboratory (SC5314) C. albicans isolate. These results indicate that despite some clinical evidence suggestive of off-target efficacy of LTRAs in RVVC, LTs and associated signaling pathways appear to be dispensable in the immunopathogenesis of VVC.

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Section: Discussionmentioning

confidence: 94%