Host defense cathelicidins in cattle: types, production, bioactive functions and potential therapeutic and diagnostic applications

Young-Speirs, Morgan; Drouin, Dominique; Cavalcante, Paloma A.; Barkema, Herman W.; Cobo, Eduardo R.

doi:10.1016/j.ijantimicag.2018.02.006

Cited by 54 publications

(30 citation statements)

References 87 publications

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“…In CHS buffalo, LL-37 was significantly more abundant than in TN buffalo (based on both LFQ and PRM), suggesting that the CHS buffalo might increase the thermotolerance to infection. As LL-37 is important for the immune and inflammatory response [31], it is possible that the higher LL-37 serum levels seen here in CHS buffalo increase the defense against microbial invasions, compensating for the relatively low level of antioxidant enzymes (GPX3).…”

Section: Discussionmentioning

confidence: 93%

Serum proteomics analysis reveals the thermal fitness of dairy buffalo to chronic heat stress

Liu

Guo

Mao

et al. 2019

Preprint

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Background: Chronic heat stress (CHS), aggravated by global warming, reduces the production efficiency of the buffalo dairy industry. CHS changes protein abundance, and low-abundant proteins take important roles in biological processes. Results: The objective of the study was to assess differences in low-abundant serum proteins in dairy buffaloes at thermoneutral (TN) or under chronic heat stress (CHS) conditions with proteomic approaches. Six dairy buffaloes as reference animal raised in TN season, and another six dairy buffaloes raised in CHS to discover the molecular mechanism of thermal fitness in hot season with serum proteomics. After the removal of multiple high-abundant proteins in serum, 344 low-abundant proteins were identified in serum with label-free quantification. Of these, 17 low-abundant differentially expressed serum proteins with known functions were detected, and five of these differentially expressed proteins were validated with parallel reaction monitoring. These five proteins were associated with various aspects of heat stress, including decreased heat production, increased blood oxygen delivery, and enhanced natural disease resistance. Conclusions: Lipase (LPL), glutathione peroxidase 3 (GPX3), cathelicidin-2 (CATHL2), ceruloplasmin (CP), and hemoglobin subunit alpha 1 (HBA1) were shown to play cooperative roles in CHS fitness in dairy buffalo. Dairy buffaloes adapt to CHS and hypoxia with high levels of RBCs, HBA1 and CP increased blood oxygen delivery capacity and thermal fitness.

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Section: Discussionmentioning

confidence: 93%

Serum proteomics analysis reveals the thermal fitness of dairy buffalo to chronic heat stress

Liu

Guo

Mao

et al. 2019

Preprint

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“…LL-37, a small cationic antimicrobial peptide produced in the epithelial cells, disrupts the activity of gram-negative bacteria by damaging and destroying bacterial membranes [ 49 , 50 ]. LL-37 plays key roles in inflammatory response regulation and immune mediator induction [51,52]. In heat-stressed mice, LL-37 production was suppressed by the nicotinic acetylcholine system, increasing the susceptibility of the mice Staphylococcus aureus [53 ].…”

Section: Discussionmentioning

confidence: 99%

“…suggesting that the HS buffalo might also be more susceptible to infection. As LL-37 is important for the immune and inflammatory response [54,55]. it is possible that the higher LL-37 serum levels seen here in HS buffalo might compensate for the relatively low GPX3 levels.…”

Section: Discussionmentioning

confidence: 99%

System-level Insights into the Adaptation of Domestic Dairy Buffalo to Chronic Heat Stress

Gu¹,

Tang²,

Fu³

et al. 2019

Preprint

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Chronic heat stress (HS), aggravated by global warming, reduces the production efficiency of the buffalo dairy industry. Here, we conducted a proteomic analysis to investigate the adaptation strategies used by buffalo in response to heat stress. Seventeen differentially abundant proteins with known functions were detected using label-free quantification (LFQ), and five of these differentially expressed proteins were validated with parallel reaction monitoring (PRM). These five proteins were associated with various aspects of heat stress, including decreased heat production, increased blood oxygen delivery, and enhanced natural disease resistance. Lipase (LPL), glutathione peroxidase 3 (GPX3), cathelicidin-2 (CATHL2, LL-37), ceruloplasmin (CP), and hemoglobin subunit alpha 1 (HBA1) were shown to play cooperative roles in the tolerance of chronic HS in dairy buffalo. We found that high levels of HBA1 increased blood oxygen transport capacity. Our results increase our understanding of the adaptation of dairy buffalo to chronic heat stress.

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“…Its murine ortholog, mCRAMP, is also an important member of this family, as evidenced by in vivo studies documenting that Cramp-deficient mice are more susceptible to bacterial infection than wild-type mice [9]. In addition, although the immune-modulatory properties remain not well understood, bovine cathelicidin peptides BMAP-27 and BMAP-34 have been shown to efficiently inhibit the growth of pathogenic bacteria [10]. Mammalian cathelicidins are highlighted as promising alternatives to traditional antibiotics for the treatment of bacterial infection [3].…”

Section: Introductionmentioning

confidence: 99%

Differential Abilities of Mammalian Cathelicidins to Inhibit Bacterial Biofilm Formation and Promote Multifaceted Immune Functions of Neutrophils

Xie

Zan

Zhang

et al. 2020

IJMS

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Mammalian cathelicidins act as the potent microbicidal molecules for controlling bacterial infection, and are considered promising alternatives to traditional antibiotics. Their ability to modulate host immune responses, as well as their bactericidal activities, is essential for therapeutic interventions. In this study, we compared the bactericidal activities, antibiofilm activities and immune-modulatory properties of cathelicidins BMAP-27, BMAP-34, mCRAMP, and LL-37, and evaluated the therapeutic efficacy of the combination of BMAP-27 and LL-37 using a mouse pulmonary infection model. Our results showed that all of the four cathelicidins effectively killed bacteria via rapid induction of membrane permeabilization, and BMAP-27 exhibited the most excellent bactericidal activity against diverse bacterial pathogens. BMAP-27, mCRAMP, and LL-37 effectively inhibited biofilm formation, while BMAP-34, mCRAMP and LL-37 exerted immunomodulatory functions with varying degrees of efficacy by stimulating the chemotaxis of neutrophils, inducing the production of reactive oxygen species, and facilitating the formation of neutrophil extracellular traps. Of note, the combination of BMAP-27 and LL-37 effectively enhanced the clearance of Pseudomonas aeruginosa and reduced the organ injury in vivo. Together, these findings highlight that identifying the appropriate synergistic combination of mammalian cathelicidins with different beneficial properties may be an effective strategy against bacterial infection.

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Host defense cathelicidins in cattle: types, production, bioactive functions and potential therapeutic and diagnostic applications

Cited by 54 publications

References 87 publications

Serum proteomics analysis reveals the thermal fitness of dairy buffalo to chronic heat stress

Serum proteomics analysis reveals the thermal fitness of dairy buffalo to chronic heat stress

System-level Insights into the Adaptation of Domestic Dairy Buffalo to Chronic Heat Stress

Differential Abilities of Mammalian Cathelicidins to Inhibit Bacterial Biofilm Formation and Promote Multifaceted Immune Functions of Neutrophils

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