Host-dependent microbes

Hanks, John H.

doi:10.1128/br.30.1.114-135.1966

Cited by 15 publications

(1 citation statement)

References 58 publications

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“…Among the factors considered responsible for the obligate parasitism may be biochemical and physiological defects. "Energy parasitism," membrane defects, or lesions in pathways of intermediary metabolism have been suggested for rickettsiae and the chlamydia (10,11,20,31). The genomic size of C. burnetii has been estimated as 1.8 x 107 daltons (26), and 109 daltons may be calculated for the genome of Rickettsia rickettsii (15).…”

Section: Resultsmentioning

confidence: 99%

Synthesis of ribonucleotides and their participation in ribonucleic acid synthesis by Coxiella burnetii

Christian¹,

Paretsky²

1977

J Bacteriol

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Synthesis of ribonucleic acid (RNA) by the deoxyribonucleic acid-dependent RNA polymerase of Coxiella burnetii required adenosine, uridine, guanosine, and cytidine 5'-triphosphates. Cell-free preparations of this obligate intracellular procaryotic parasite had competence to phosphorylate ribonucleoside mono- and diphosphates in the presence of exogenous adenosine and guanosine 5'-triphosphates to the corresponding di- and triphosphates. C. burnetii contained about 2 nmol of adenosine 5'-triphosphate per mg of protein, which could serve as a approximately P donor for in vivo synthesis of nucleoside triphosphates. The latter were then used as substrates in the synthesis of RNA in a coordinated metabolic system with C. burnetii RNA polymerase. It is suggested that during infection the rickettsiae might obtain the nucleotides necessary for RNA synthesis from the vacuoles in which C. burnetii proliferates.

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