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Many of tlie cliaracteristics axid potentialitics of chick tissue cultures are continually being recognized, aiid adapted to experimental purposes. Their remarkable ability, under appropriate circumstances, to subsist without replacemcnt of the nutrient medium deserves widcr recognition. This property may be used not only to facilitate the maintenance of stock cultures, but also niay be exploited in the study of cell iiutrition and other problems. Parker ('38) mentions that cultures will rcmain alive at room temperatures for several weeks.Saiiders ( '40), maintained guinea pig embryonic brain in Simms' serum nltrafiltrate for TO days at 23"C., stated that Simnis demonstrated viability in adult chicken aorta cultures after 47 clays a t room temperature.In studying the possibility of using chick tissue culturcs as host cells f o r the propagation of leprosy bacili, it was found that tlie rapid growth arid division of the cultures undcr the classical conditioiis of cultivatioiz resulted in early disappearance of the bacilli by simple dilution. The extremely sluggish developmerit of leprosy, even in susccptiblc individuals, made it desirable to maintain cell populations in as stable a conclitiori as possible. It was incidental to such it-ork that the present observations were made. Under the more f avorahle of the conditioiis to be described, 2 drops of imbcddiiig coagnPresent address : Leonard Wood Memorial nepartinent of Racteriology aiid Immunology, Harvard Medical School, 25 Shattuek St., Boston 15, Massaehusetts. 235

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