Sterilization of Skin

Hanks, John H.; Wallace, Roslyn E.

doi:10.3181/00379727-71-17132

Cited by 53 publications

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“…Foetal rats were delivered by Caesarian section, livers were removed aseptically and rinsed free of blood in balanced salts solution (Hanks & Wallace, 1949) and dried on sterile paper napkins. Livers were chopped on a Mickle Chopper (The Mickle Laboratories Engineering Co., Gomshall, Surrey, U.K.) set at 0.1 mm using two traverses, one perpendicular to the other.…”

Section: Methodsmentioning

confidence: 99%

Tyrosine aminotransferase induction in hepatocytes cultured from rat foetuses treated with dexamethasone in utero

Yeoh¹,

Arbuckle²,

Oliver³

1979

Biochemical Journal

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1. The administration of dexamethasone to foetal rats in utero does not result in the appearance of specific tyrosine aminotransferase activity even after 24h. 2. When foetal hepatocytes are cultured in vitro from animals treated in utero with dexamethasone, significantly higher activities of specific tyrosine aminotransferase are found than in untreated controls. 3. Dexamethasone in vitro induces specific tyrosine aminotransferase in cells cultured from control animals, and the effect is maximal at 10nM in the culture medium. 4. Actinomycin D at 0.2,ug/ml in the culture medium completely prevents the induction of activity in vitro. 5. In cultures established from animals treated with dexamethasone in utero, the increase in specific tyrosine aminotransferase activity over the control cultures is only marginally decreased in the presence of actinomycin D. 6. The results can be interpreted to mean that dexamethasone in utero stimulates the transcription of enzyme-specific mRNA, which is not translated until a translational block in the foetal liver is removed by the conditions of culture in vitro.

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Section: Methodsmentioning

confidence: 99%

Tyrosine aminotransferase induction in hepatocytes cultured from rat foetuses treated with dexamethasone in utero

Yeoh¹,

Arbuckle²,

Oliver³

1979

Biochemical Journal

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“…About 90% of the cells were intact as measured by trypan blue exclusion. The cells were incubated at 23°C in calcium-and glucose-free Hanks medium [9] [13].…”

Section: Methodsmentioning

confidence: 99%

Calcium‐mediated alterations in the oxidation—reduction state of pyridine nucleotides in isolated liver cells

Ontko

Otto

1975

FEBS Letters

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“…The isolated lymphocytes were washed twice with Hank's balanced salt solution [7]. In order to remove the contaminating monocytes, 1 × 107 lymphocytes were suspended in 1 ml of a modified RPMI-1640 solution and kept overnight in polycarbonate dishes (pH 7.4, 20°C).…”

Section: Methodsmentioning

confidence: 99%

Ca²⁺ uptake and IP₃‐induced Ca²⁺ release in permeabilized human lymphocytes

Eberl

Schnell

1987

FEBS Letters

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The 4SCa2 + uptake and 4sCa2 + release in saponin-permeabilized human lymphocytes were studied. An ATPdependent Ca 2 + uptake into a nonmitochondrial, intracellular Ca 2 + store is observed which is approx. 2 orders of magnitude greater than the ATP-independent Ca 2 + uptake. The Ca 2 + uptake is inhibited by vanadate, but it is insensitive to oligomycin and ruthenium red. IP 3 induces dose-dependent 4sCa2 + release. For half-maximum Ca 2 + release 0.25-0.5 pM IP 3 is required. The results of our studies suggest that 45Ca2 + is predominantly stored within the endoplasmic reticulum of the lymphocytes.

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Sterilization of Skin

Cited by 53 publications

References 0 publications

Tyrosine aminotransferase induction in hepatocytes cultured from rat foetuses treated with dexamethasone in utero

Tyrosine aminotransferase induction in hepatocytes cultured from rat foetuses treated with dexamethasone in utero

Calcium‐mediated alterations in the oxidation—reduction state of pyridine nucleotides in isolated liver cells

Ca²⁺ uptake and IP₃‐induced Ca²⁺ release in permeabilized human lymphocytes

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Sterilization of Skin

Cited by 53 publications

References 0 publications

Tyrosine aminotransferase induction in hepatocytes cultured from rat foetuses treated with dexamethasone in utero

Tyrosine aminotransferase induction in hepatocytes cultured from rat foetuses treated with dexamethasone in utero

Calcium‐mediated alterations in the oxidation—reduction state of pyridine nucleotides in isolated liver cells

Ca2+ uptake and IP3‐induced Ca2+ release in permeabilized human lymphocytes

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Ca²⁺ uptake and IP₃‐induced Ca²⁺ release in permeabilized human lymphocytes