Host environment as a modulating factor of swine natural killer cell activity

Richerson, Joan T.; Misfeldt, Michael L.

doi:10.1016/0165-2427(89)90143-8

Cited by 16 publications

(9 citation statements)

References 17 publications

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“…4 and 5 ). This is contrary to recent findings by Richerson and Misfeldt (1989) in Sinclair swine, where in vitro NK-CMC is suggested to correlate with stages of tumor progression or regression.…”

Section: Release Of Interferon Upon Contact Of Lymphocytes With Targecontrasting

confidence: 99%

Natural killer (NK) activity of porcine blood lymphocytes against allogeneic melanoma target cells

Büttner

Wanke²,

Obermann

1991

Veterinary Immunology and Immunopathology

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Allogeneic PM/86 melanoma cells of Munich Troll miniature swine have been used for the demonstration of porcine peripheral blood NK cell activity. Compared with the specific lysis of xenogeneic K562-, U937- and Vero-target cells, NK cell-mediated cytotoxicity (NK-CMC) against PM/86 melanoma tumor cells was significantly lower in a 16 h chromium release assay. The target cell susceptibility to peripheral blood NK-CMC of both adult Troll miniature swine and German Landrace sows was very similar. Cold target inhibition assays revealed the allogeneic PM/86 melanoma cells to be the most powerful inhibitors of NK-CMC. Nylon wool non-adherent lymphocytes produced interferon (IFN)-alpha in different quantities upon contact with NK susceptible target cells. The NK effector cells could be stimulated to a higher lytic activity against all susceptible targets by a moderate dose of natural human interleukin-2 (nhuIL-2). The role of NK-CMC in melanoma tumor rejection and/or prevention of metastases is yet unknown in swine although porcine melanoma serves as a good model for the disease in man.

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Section: Release Of Interferon Upon Contact Of Lymphocytes With Targecontrasting

confidence: 99%

Natural killer (NK) activity of porcine blood lymphocytes against allogeneic melanoma target cells

Büttner

Wanke²,

Obermann

1991

Veterinary Immunology and Immunopathology

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“…Others (Norley and Wardley, 1983;Richerson and Misfeldt, 1989) have shown an association between in vivo infection with African Swine-Fever virus and suppressed NK activity. Exposure of immunologically competent pigs (12-16 weeks of age) to multiple pathogens in the form of vaccines significantly increased NK activity (Richerson and Misfeldt, 1989). These studies thus indicated that NK cells may be relevant in mediating anti-microbial and antiviral responses.…”

Section: Target Cellsmentioning

confidence: 95%

Natural killer (NK) cells in domestic animals: Phenotype, target cell specificity and cytokine regulation

Evans

Jaso-Friedmann

1993

Vet Res Commun

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A comprehensive review of NK cells in animals of veterinary medical importance has not been previously published. However, these cells have a high level of immunological/medical relevance due to their role in tumour cell destruction and B-cell regulation, as well as their inhibitory activities against various parasites and bacteria. In the present review, NK cells from agriculturally important animals are characterized. Cell phenotype descriptions have shown that for each species, unique (i.e. non-cross-reactive with anti-human CD antibodies) and different monoclonals are required to identify NK cells. These cells lyse certain tumour and virus transformed target cells and, as might be expected from the diverse species compared in this review, analysis of the tissue distribution of NK cells gives highly varied results. NK cell morphology differs in these species from small agranular to large granular lymphocytes. The final area considered relates to studies on the effects of cytokines on NK function and to research identifying which cytokines (if any) are produced by NK cells during activation responses. The largest quantity of available data concerns functional and descriptive studies in these animals. However, it is appropriate to consider this research as a starting point on which to build comparative and molecular studies of the roles of NK cells in immunosurveillance and immunoregulation.

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“…For porcine NK cells a perforin + CD2 + CD3 -CD4 -CD5 -CD6 -CD8α + CD8ß -CD11b + CD16 + phenotype has been described and it was shown that these lymphocytes can perform immediate cytotoxicity against NK-susceptible targets [11][12][13]. Moreover, in parasitic as well as in viral infections increases in NK cell number and activity have been reported [14,15], but also inhibitory effects on NKcell mediated cytotoxicity and cytokine production by viral infections are described [16][17][18][19]. Despite these hints on important functions of porcine NK cells in vivo, so far no investigations on the existence of functionally differing NK-cell subsets have been reported.…”

Section: Introductionmentioning

confidence: 99%

Porcine CD8αdim/-NKp46high NK cells are in a highly activated state

Mair

Müllebner

Essler

et al. 2013

Vet Res

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Natural Killer (NK) cells play a crucial role in the early phase of immune responses against various pathogens. In swine so far only little information about this lymphocyte population exists. Phenotypical analyses with newly developed monoclonal antibodies (mAbs) against porcine NKp46 recently revealed that in blood NKp46- and NKp46+ cells with NK phenotype exist with comparable cytotoxic properties. In spleen a third NKp46-defined population with NK phenotype was observed that was characterised by a low to negative CD8α and increased NKp46 expression. In the current study it is shown that this NKp46high phenotype was correlated with an increased expression of CD16 and CD27 compared to the CD8α+NKp46- and NKp46+ NK-cell subsets in spleen and blood. Additionally NKp46high NK cells expressed elevated levels of the chemokine receptor CXCR3 on mRNA level. Functional analyses revealed that splenic NKp46high NK cells produced much higher levels of Interferon-γ and Tumor Necrosis Factor-α upon stimulation with cytokines or phorbol-12-myristate-13-acetate/Ionomycin compared to the other two subsets. Furthermore, cross-linking of NKp46 by NKp46-specific mAbs led to a superior CD107a expression in the NKp46high NK cells, thus indicating a higher cytolytic capacity of this subset. Therefore porcine splenic NKp46high NK cells represent a highly activated subset of NK cells and may play a profound role in the immune surveillance of this organ.

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Host environment as a modulating factor of swine natural killer cell activity

Cited by 16 publications

References 17 publications

Natural killer (NK) activity of porcine blood lymphocytes against allogeneic melanoma target cells

Natural killer (NK) activity of porcine blood lymphocytes against allogeneic melanoma target cells

Natural killer (NK) cells in domestic animals: Phenotype, target cell specificity and cytokine regulation

Porcine CD8αdim/-NKp46high NK cells are in a highly activated state

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