Host Lipids in Positive-Strand RNA Virus Genome Replication

Zhang, Zhenlu; He, Guijuan; Filipowicz, Natalie; Randall, Glenn; Belov, George A.; Kopek, Benjamin G.; Wang, Xiaofeng

doi:10.3389/fmicb.2019.00286

Cited by 118 publications

(109 citation statements)

References 188 publications

(282 reference statements)

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“…For example, (+)ssRNA viruses require the host translation machinery to synthesize the proteins necessary for their replication. These proteins are then assembled in a membrane-associated replication complex that replicates viral RNA ( Zhang et al, 2019 ). Most (+)ssRNA virus replication complexes are associated with the endoplasmic reticulum (ER), although this can also occur on the mitochondrial outer membrane (OMM) in Flock house virus ( Miller and Ahlquist, 2002 ; Virgin et al, 2007 ).…”

Section: Sars-c O V-2 Structure and Replication Cymentioning

confidence: 99%

“…A number of (+)ssRNA viruses require lipid metabolism for viral genome replication and virions assembly ( Zhang et al, 2019 ). In fact, mitochondrial β-oxidation is essential to replenish metabolic intermediates and to produce the energy required for HCV replication ( Diamond et al, 2010 ; Rasmussen et al, 2011 ).…”

Section: Viral Control Of Mitochondrial Dynamics and Metabolismmentioning

confidence: 99%

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Mitochondria Targeted Viral Replication and Survival Strategies—Prospective on SARS-CoV-2

et al. 2020

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SARS-CoV-2 is a positive sense RNA coronavirus that constitutes a new threat for the global community and economy. While vaccines against SARS-CoV-2 are being developed, the mechanisms through which this virus takes control of an infected cell to replicate remains poorly understood. Upon infection, viruses completely rely on host cell molecular machinery to survive and replicate. To escape from the immune response and proliferate, viruses strategically modulate cellular metabolism and alter subcellular organelle architecture and functions. One way they do this is by modulating the structure and function of mitochondria, a critical cellular metabolic hub but also a key platform for the regulation of cellular immunity. This versatile nature of mitochondria defends host cells from viruses through several mechanisms including cellular apoptosis, ROS signaling, MAVS activation and mitochondrial DNA-dependent immune activation. These events are regulated by mitochondrial dynamics, a process by which mitochondria alter their structure (including their length and connectivity) in response to stress or other cues. It is therefore not surprising that viruses, including coronaviruses hijack these processes for their survival. In this review, we highlight how positive sense RNA viruses modulate mitochondrial dynamics and metabolism to evade mitochondrial mediated immune response in order to proliferate.

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Section: Sars-c O V-2 Structure and Replication Cymentioning

confidence: 99%

Section: Viral Control Of Mitochondrial Dynamics and Metabolismmentioning

confidence: 99%

Mitochondria Targeted Viral Replication and Survival Strategies—Prospective on SARS-CoV-2

et al. 2020

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“…(+)RNA viruses can also manipulate the host cell lipid and cholesterol trafficking machinery to produce RO membranes with much higher phosphatidylinositol 4phosphate (PI(4)P) and cholesterol content than the host ER/Golgi membranes from which they are derived. To this end, many enteroviruses exploit the PI(4)P-driven cholesterol transport system normally used at ER MCSs, via recruitment of OSBP/OSBPL proteins to the RO membrane [21][22][23][24] . The abundant interactions of SARS-CoV-2 proteins with host MCS components and lipid/cholesterol transfer proteins strongly suggests that this virus manipulates lipid trafficking functions in a similar manner.…”

Section: Suppmentioning

confidence: 99%

A SARS-CoV-2 BioID-based virus-host membrane protein interactome and virus peptide compendium: new proteomics resources for COVID-19 research

St-Germain

Astori

Samavarchi-Tehrani

et al. 2020

Preprint

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SummaryKey steps of viral replication take place at host cell membranes, but the detection of membrane-associated protein-protein interactions using standard affinity-based approaches (e.g. immunoprecipitation coupled with mass spectrometry, IP-MS) is challenging. To learn more about SARS-CoV-2 - host protein interactions that take place at membranes, we utilized a complementary technique, proximity-dependent biotin labeling (BioID). This approach uncovered a virus-host topology network comprising 3566 proximity interactions amongst 1010 host proteins, highlighting extensive virus protein crosstalk with: (i) host protein folding and modification machinery; (ii) membrane-bound vesicles and organelles, and; (iii) lipid trafficking pathways and ER-organelle membrane contact sites. The design and implementation of sensitive mass spectrometric approaches for the analysis of complex biological samples is also important for both clinical and basic research proteomics focused on the study of COVID-19. To this end, we conducted a mass spectrometry-based characterization of the SARS-CoV-2 virion and infected cell lysates, identifying 189 unique high-confidence virus tryptic peptides derived from 17 different virus proteins, to create a high quality resource for use in targeted proteomics approaches. Together, these datasets comprise a valuable resource for MS-based SARS-CoV-2 research, and identify novel virus-host protein interactions that could be targeted in COVID-19 therapeutics.

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“…Indeed, viral proteins modulating membrane curvature were also shown to promote the formation of membrane contact sites and the recruitment of host factors involved in lipid metabolism (van der Schaar et al 2016), jn particular phosphatidylinositol-4-phosphate (PI4P) or phosphatidylethanolamine (PE) synthesis (Altan-Bonnet 2017). Interestingly, the accumulation of PIP4 or PE is often accompanied with an enrichment in sterol that might contribute to the stabilization of membrane curvature and is important for the replication of the virus (Zhang et al 2019).…”

Section: Hijacking Membrane Remodeling: Lessons Learned From Viral Inmentioning

confidence: 99%

Intracellular Membranes: Conserved Mechanisms of Formation and Regulation Throughout Evolution

Mir¹,

Biou²,

Dautin³

et al. 2020

Preprint

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Membrane remodeling and phospholipid biosynthesis are normally tightly regulated to maintain the shape and function of cells. Indeed, different physiological mechanisms ensure a precise coordination between de novo phospholipid biosynthesis and modulation of membrane morphology. Interestingly, the overproduction of certain membrane proteins hijack these regulation networks, leading to the formation of impressive intracellular membrane structures in both prokaryotic and eukaryotic cells. The proteins triggering membrane proliferation share two major common features: 1) they promote the formation of highly curved membrane domains and 2) they lead to an enrichment in anionic, cone-shaped phospholipids (cardiolipin or phosphatidic acid) in the newly formed membranes. Taking into account the available examples of membrane proliferation upon protein overproduction, together with the latest biochemical, biophysical and structural data, we explore the relationship between protein synthesis and membrane biogenesis. We propose a mechanism for the formation of these non-physiological intracellular membranes that shares similarities with natural inner membrane structures found in α-proteobacteria, mitochondria and some viruses-infected cells, pointing towards a conserved feature through evolution. We hope that the information discussed in this review will give a better grasp of the biophysical mechanisms behind physiological and induced intracellular membrane proliferation, inspiring new biotechnological applications.

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Host Lipids in Positive-Strand RNA Virus Genome Replication

Cited by 118 publications

References 188 publications

Mitochondria Targeted Viral Replication and Survival Strategies—Prospective on SARS-CoV-2

Mitochondria Targeted Viral Replication and Survival Strategies—Prospective on SARS-CoV-2

A SARS-CoV-2 BioID-based virus-host membrane protein interactome and virus peptide compendium: new proteomics resources for COVID-19 research

Intracellular Membranes: Conserved Mechanisms of Formation and Regulation Throughout Evolution

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