Host plant odours and their recognition by the odourant‐binding proteins of <scp><i>Diaphorina citri</i></scp> Kuwayama (Hemiptera: Psyllidae)

Zheng-bing, Wang; Gao, Chuanhe; Liu, Jiali; Zhou, Wenwu; Zeng, Xinnian

doi:10.1002/ps.5786

Cited by 15 publications

(17 citation statements)

References 68 publications

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“…Multiple strategies may be applied, such as the application of specific VOCs to attract or repel insects, or the dysfunction of OBPs through the key binding sites of VOCs. These universal strategies may be useful in attempts to manage the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), which prefers to lay eggs on young citrus leaves and is the vector of Candidatus Liberibacter asiaticus causing Huanglongbing 73,74 …”

Section: Discussionmentioning

confidence: 99%

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Characterization of the binding ability of the odorant binding protein BminOBP9 of Bactrocera minax to citrus volatiles

Yao

Zhao

Zhong

et al. 2020

Pest Management Science

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BACKGROUND Bactrocera minax, one of the most important citrus pests, oviposits exclusively on citrus fruit. In the insect olfactory system, odorant‐binding proteins (OBPs) facilitate the initial recognition role of host odor molecules. The aim of this study was to characterize the functional OBPs of B. minax and identify specific volatile organic compounds in the Citrus genus as OBP targets. RESULTS BminOBP9 (BminGOBP99a), a closely related homolog of BdorGOBP99a, which reduces the egg‐laying behavior of Bactrocera dorsalis through silencing technology, was cloned, expressed, and purified. The binding ability of BminOBP9 to 11 citrus volatiles was then examined using fluorescence competition binding assays (FCBA). The results demonstrated that BminOBP9 could bind to all tested citrus volatiles, as could BdorGOBP99a, ZcucGOBP99a, and ZtauGOBP99a. Interestingly, the binding ability of BminOBP9 was the strongest among the four, suggesting that BminOBP9 may have a function in the specific recognition of citrus volatiles. Furthermore, we aligned the above four proteins and found nine distinctive amino acid sites in BminOBP9. To identify the unique binding sites of BminOBP9, we produced the nine mutants using site‐directed mutagenesis. Further FCBA showed that the binding ability of the nine mutants to citrus volatiles significantly reduced, and six of them (substitutes S24P, L36F, E53K, N68D, D112A, and S118R) had the weakest binding ability. CONCLUSION The results demonstrated that BminOBP9 was the specific protein involved in the perception of citrus host volatiles by B. minax. Moreover, BminOBP9 could prove efficient in screening the candidate odors for pest management. © 2020 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

“…These universal strategies may be useful in attempts to manage the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), which prefers to lay eggs on young citrus leaves and is the vector of Candidatus Liberibacter asiaticus causing Huanglongbing. 73,74…”

Section: Wileyonlinelibrarycom/journal/psmentioning

confidence: 99%

Characterization of the binding ability of the odorant binding protein BminOBP9 of Bactrocera minax to citrus volatiles

Yao

Zhao

Zhong

et al. 2020

Pest Management Science

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“…The tissue‐specific expression patterns of OBP‐encoding genes in insects are usually related to their physiological functions 26 . OBP6 exhibited high expression in the antennae of D. citri and stronger binding affinity to some host plant volatiles 25,26 . Our previous studies have shown that AbamOBP1, AbamOBP24, and AbamOBP30, have evident male antenna‐specific expression patterns 40 .…”

Section: Discussionmentioning

confidence: 99%

“…OBP14 in Cnaphalocrocis medinalis Güenée (Lepidoptera: Pyralidae) was found to mediate the recognition ability for nerolidol and β‐ionone 24 . Some OBPs in Diaphorina citri are involved in the perception of some important host plant odorants such as β‐caryophyllene and some terpenoids 25,26 …”

Section: Introductionmentioning

confidence: 99%

Discovery of behaviorally active semiochemicals in Aenasius bambawalei using a reverse chemical ecology approach

Yang

Duan

et al. 2021

Pest Management Science

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BACKGROUND The invasive mealybug, Phenacoccus solenopsis, has caused serious damage to cotton crops throughout the world. Aenasius bambawalei is a dominant endoparasitoid of P. solenopsis. Exploration of behaviorally active semiochemicals may promote the efficacy of parasitoids used in biological control. Reverse chemical ecology, based on the physiological function of odorant‐binding proteins (OBPs), provides an effective approach to screen behaviorally active compounds to target insect pests. Determination of the binding mechanisms and specificity towards different odorants in A. bambawalei may facilitate the development of more‐efficient biological control strategies. RESULTS We characterized the expression profile and analyzed the binding affinity of OBP28 in A. bambawalei. AbamOBP28 showed high expression in the wings and antennae of both male and female A. bambawalei. A fluorescence competitive binding assay indicated that AbamOBP28 displayed strong binding affinity to most candidate ligands. Circular dichroism spectra demonstrated that 1‐octen‐3‐one, myrcene, dodecane, 2,4,4‐trimethyl‐2‐pentene, nonanal, and limonene elicited conformational changes in AbamOBP28. Electrophysiological and behavioral bioassays revealed that diethyl sebacate, 2,4,4‐trimethyl‐2‐pentene, and 1‐octen‐3‐one evoked significant electroantennography responses and functioned as attractants in A. bambawalei at specific concentrations. Furthermore, three‐dimensional structure modeling and molecular docking showed that hydrogen bonds were formed by Glu1 and Ser75 of AbamOBP28 with diethyl sebacate, respectively. CONCLUSION These results demonstrate that AbamOBP28 is involved in the chemoreception of A. bambawalei. The identified protein provides a potential target for efficient enemy utilization and pest control, and the overall results may help develop protocols for more effective screening of behaviorally active semiochemicals. © 2021 Society of Chemical Industry

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“…There are many methods and devices that have been reported to extract and analyze VOCs in plant leaves, such as simultaneous distillation–extraction (SDE) [ 20 ], steam distillation (SD) [ 21 ], and supercritical fluid extraction (SFE) [ 22 ]. Wang et al analyzed the components of volatiles from the new shoots of six healthy host plant species via the headspace solid-phase microextraction (HS-SPME) method and showed that β-caryophyllene was the characteristic VOC in flushing shoots of the tested ACP host plant species [ 23 ]. However, they did not analyze VOCs from HLB-infected species.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Volatile Organic Compounds of Healthy and Huanglongbing-Infected Navel Orange and Pomelo Leaves by HS-GC-IMS

et al. 2020

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The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is the only natural vector of bacteria responsible for Huanglongbing (HLB), a worldwide destructive disease of citrus. ACP reproduces and develops only on the young leaves of its rutaceous host plants. Olfactory stimuli emitted by young leaves may play an important role in ACP control and HLB detection. In this study, volatile organic compounds (VOCs) from healthy and HLB-infected young leaves of navel orange and pomelo were analyzed by headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS). A total of 36 compounds (including dimers or polymers) were identified and quantified from orange and 10 from pomelo leaves. Some compounds showed significant differences in signal intensity between healthy and HLB-infected leaves and may constitute possible indicators for HLB infection. Principal component analysis (PCA) clearly discriminated healthy and HLB-infected leaves in both orange and pomelo. HS-GC-IMS was an effective method to identify VOCs from leaves. This study may help develop new methods for detection of HLB or find new attractants or repellents of ACP for prevention of HLB.

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Host plant odours and their recognition by the odourant‐binding proteins of Diaphorina citri Kuwayama (Hemiptera: Psyllidae)

Cited by 15 publications

References 68 publications

Characterization of the binding ability of the odorant binding protein BminOBP9 of Bactrocera minax to citrus volatiles

Characterization of the binding ability of the odorant binding protein BminOBP9 of Bactrocera minax to citrus volatiles

Discovery of behaviorally active semiochemicals in Aenasius bambawalei using a reverse chemical ecology approach

Characterization of Volatile Organic Compounds of Healthy and Huanglongbing-Infected Navel Orange and Pomelo Leaves by HS-GC-IMS

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