Host Restriction of
            <i>Salmonella enterica</i>
            Serotype Typhimurium Pigeon Isolates Does Not Correlate with Loss of Discrete Genes

Andrews‐Polymenis, Helene; Rabsch, Wolfgang; Porwollik, Steffen; McClelland, Michael; Rosetti, Carlos; Adams, L. Garry; Bäumler, Andreas J.

doi:10.1128/jb.186.9.2619-2628.2004

Cited by 29 publications

(29 citation statements)

References 28 publications

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“…As they evolve toward host specificity, more genomic rearrangements will be observed. Elegant studies previously performed on pigeon-associated serovar Typhimurium indicate that these strains are beginning to pursue a divergent evolutionary path relative to the more common generalist serovar Typhimurium strains (1,15,19). Results obtained in this study confirm this.…”

supporting

confidence: 81%

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Pigeon-Associated Strains of Salmonella enterica Serovar Typhimurium Phage Type DT2 Have Genomic Rearrangements at rRNA Operons

et al. 2004

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confidence: 81%

“…The observation that Fels-1 and Fels-2 are missing from these strains is not surprising, as similar results have been found in other serovar Typhimurium strains recently (1,17). No genomic differences between strains with the inversion and the strain lacking the inversion were detected.…”

supporting

confidence: 59%

Pigeon-Associated Strains of Salmonella enterica Serovar Typhimurium Phage Type DT2 Have Genomic Rearrangements at rRNA Operons

et al. 2004

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“…The set of signal intensities for each gene and strain (denoted P gs , with a gene range of [1,4048] for g and a serovar range of [1,40] for s) was used to calculate the mean normalized signal intensity of P gs (denoted as ) 2 was plotted in order of increasing variance (Fig. 1a), and using these values as estimators for gene variability, the 4,048 genes for which data were available from all strains were sorted using the decision tree illustrated in Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Microarray genome comparison of the host-restricted S. enterica serovar Typhimurium DT2 and DT99 pigeon isolate genomes to the broad-host-range S. enterica serovar Typhimurium LT2 genome has shown no genetic islands, present in LT2, whose loss could be associated with host restriction (1). Similarly, in this study, we were also unable to distinguish the genomes of two S. enterica serovar Typhimurium pigeon isolates, S6332 and S1055, from those of the remaining S. enterica serovar Typhimurium strains.…”

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confidence: 99%

Identification of Core and Variable Components of the Salmonella enterica Subspecies I Genome by Microarray

et al. 2005

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We have performed microarray hybridization studies on 40 clinical isolates from 12 common serovars within Salmonella enterica subspecies I to identify the conserved chromosomal gene pool. We were able to separate the core invariant portion of the genome by a novel mathematical approach using a decision tree based on genes ranked by increasing variance. All genes within the core component were confirmed using available sequence and microarray information for S. enterica subspecies I strains. The majority of genes within the core component had conserved homologues in Escherichia coli K-12 strain MG1655. However, many genes present in the conserved set which were absent or highly divergent in K-12 had close homologues in pathogenic bacteria such as Shigella flexneri and Pseudomonas aeruginosa. Genes within previously established virulence determinants such as SPI1 to SPI5 were conserved. In addition several genes within SPI6, all of SPI9, and three fimbrial operons (fim, bcf, and stb) were conserved within all S. enterica strains included in this study. Although many phage and insertion sequence elements were missing from the core component, approximately half the pseudogenes present in S. enterica serovar Typhi were conserved. Furthermore, approximately half the genes conserved in the core set encoded hypothetical proteins. Separation of the core and variant gene sets within S. enterica subspecies I has offered fundamental biological insight into the genetic basis of phenotypic similarity and diversity across S. enterica subspecies I and shown how the core genome of these pathogens differs from the closely related E. coli K-12 laboratory strain.

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“…NC_003197) and additional probes derived from the serovar Typhi CT18 genome (GenBank accession no. NC_003198) and other Salmonella sequences from public databases have been designed (2,15,30). Hybridization of chromosomal DNA from standard Salmonella enterica laboratory collections allowed discrimination of a total of Ͼ100 strains of different S. enterica subspecies, various subspecies 1 serovars, and even different strains of the same serovar (29).…”

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confidence: 99%

Pilot Study To Evaluate Microarray Hybridization as a Tool for Salmonella enterica Serovar Typhimurium StrainDifferentiation

et al. 2005

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In developed countries, Salmonella enterica subspecies 1 serovars Enteritidis and Typhimurium range among the most common causes of bacterial food-borne infections. The surveillance and typing of epidemic Salmonella strains are important tools in epidemiology. Usually, Salmonella enterica subspecies 1 serovars are differentiated by serotyping for diagnostic purposes. Further differentiation is done by phage typing as well as molecular typing techniques. Here we have designed and evaluated a prototype DNA microarray as a tool for serovar Typhimurium strain differentiation. It harbors 83 serovar Typhimurium probes obtained by differential subtractive hybridization and from the public database. The microarray yielded reproducible hybridization patterns in repeated hybridizations with chromosomal DNA of the same strain and could differentiate five serovar Typhimurium reference strains (DT204, DT104, DT208, DT36, and LT2). Furthermore, the microarray identified two distinct groups among 13 serovar Typhimurium DT104 strains. This correlated with observations from pulsed-field gel electrophoresis analysis. Twenty-three further serovar Typhimurium strains were analyzed to explore future directions for optimization of the simple 83-probe DNA microarray. The data presented here demonstrate that DNA microarrays harboring small numbers of selected probes are promising tools for serovar Typhimurium strain typing.Salmonella spp. are pathogenic enterobacteria which can cause food-borne diseases ranging from a mild gastroenteritis to systemic infections (typhoid fever). In developed countries, infections with Salmonella enterica subspecies 1 serovars Enteritidis and Typhimurium range among the most common causes of bacterial food-borne infections.For diagnostic purposes, Salmonella enterica subspecies 1 serovars are differentiated by serotyping (28), which allows reliable identification of organisms that cause infections associated with higher risks (i.e., serovar Typhi). For epidemiological surveys, detection of outbreaks, and uncovering of the chains of transmission, serotyping is insufficient because serovar Enteritidis and serovar Typhimurium often account for Ͼ80% of all human salmonelloses. For further differentiation, serotyping is complemented by phage typing as well as molecular typing techniques.During the past decades serovar Typhimurium strains have been differentiated by phage typing (1). The Anderson phage typing scheme is based on the empirical observation that certain phages can cause lytic infections in one serovar Typhimurium strain but not another. A set of 34 bacteriophages allows more than 200 patterns of resistance or sensitivity, the "phage types" (definitive phage types [DTs]), to be distinguished. Phage typing has proven very useful for distinguishing many serovar Typhimurium strains. It has been invaluable for surveillance of the emergence and spread of epidemic serovar Typhimurium strains (i.e., DT204c and DT104) during the past decades (23,40,(42)(43)(44)49).In spite of its success, phage typing has ...

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Host Restriction of Salmonella enterica Serotype Typhimurium Pigeon Isolates Does Not Correlate with Loss of Discrete Genes

Cited by 29 publications

References 28 publications

Pigeon-Associated Strains of Salmonella enterica Serovar Typhimurium Phage Type DT2 Have Genomic Rearrangements at rRNA Operons

Pigeon-Associated Strains of Salmonella enterica Serovar Typhimurium Phage Type DT2 Have Genomic Rearrangements at rRNA Operons

Identification of Core and Variable Components of the Salmonella enterica Subspecies I Genome by Microarray

Pilot Study To Evaluate Microarray Hybridization as a Tool for Salmonella enterica Serovar Typhimurium StrainDifferentiation

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