Host status of wild roe deer in bovine tuberculosis endemic areas

Mycobacterium bovis Infection of Red Fox, France

“…In 2015, we conducted a small-scale investigation on trapped red foxes (n = 6) in a municipality of Dordogne, a TB-endemic region in southwestern France, where multihost cycles involving cattle, badgers, wild boar, and even red deer and roe deer ( Capreolus capreolus ) exist ( 6 ). The necropsy examination included detailed macroscopic inspection of lymph nodes and abdominal and thoracic viscera ( 6 ).…”

“…In 2015, we conducted a small-scale investigation on trapped red foxes (n = 6) in a municipality of Dordogne, a TB-endemic region in southwestern France, where multihost cycles involving cattle, badgers, wild boar, and even red deer and roe deer ( Capreolus capreolus ) exist ( 6 ). The necropsy examination included detailed macroscopic inspection of lymph nodes and abdominal and thoracic viscera ( 6 ). We found no TB-like visible lesions; pooled tissue samples (retropharyngeal, tracheobronchial, mediastinal, and mesenteric lymph nodes) were submitted bacterial culture and molecular diagnosis ( 6 ); urine, feces, and oropharyngeal swabs were taken, and DNA was extracted as previously described ( 7 ).…”

“…The necropsy examination included detailed macroscopic inspection of lymph nodes and abdominal and thoracic viscera ( 6 ). We found no TB-like visible lesions; pooled tissue samples (retropharyngeal, tracheobronchial, mediastinal, and mesenteric lymph nodes) were submitted bacterial culture and molecular diagnosis ( 6 ); urine, feces, and oropharyngeal swabs were taken, and DNA was extracted as previously described ( 7 ). We performed molecular diagnostic testing using MTBC PCR (IS 6110 and IS 1081 ) and the regions of difference and through spoligotyping ( 6 ), the latter 2 enabling differentiation of the MTBC members when used on highly concentrated DNAs.…”

“…We found no TB-like visible lesions; pooled tissue samples (retropharyngeal, tracheobronchial, mediastinal, and mesenteric lymph nodes) were submitted bacterial culture and molecular diagnosis ( 6 ); urine, feces, and oropharyngeal swabs were taken, and DNA was extracted as previously described ( 7 ). We performed molecular diagnostic testing using MTBC PCR (IS 6110 and IS 1081 ) and the regions of difference and through spoligotyping ( 6 ), the latter 2 enabling differentiation of the MTBC members when used on highly concentrated DNAs. Of the 6 red foxes, 4 were bacteriology positive; molecular tools enabled identification of M. bovis spoligotype SB0120 and MTBC or M. bovis excretion in feces of all infected animals (Table).…”

The VetMAXTM M. tuberculosis Complex PCR Kit detects MTBC DNA in antemortem and postmortem samples from White rhinoceros (Ceratotherium simum), African elephants (Loxodonta africana) and African buffaloes (Syncerus caffer).

“…To date, the largest reported study for the use of a standardised commercial RT-qPCR in cattle was the use of the VetMAX TM MTBC qPCR Kit using a Bayesian approach and reported a sensitivity of 87.7% and a speci city of 97% (13). Since then, this RT-qPCR has been used extensively in wildlife like wild boars, badgers, and deer and its use has drastically improved bTB surveillance programs in France (16)(17)(18).…”