Menstrual hygiene maintenance is very low cost and reliable in Pakistan for adolescents’ girls to prevent any dangerous future consequences. Adolescent girl’s good awareness and practices is very important in smooth handling and maintain menstrual hygiene. Objective: To assess the effect of educational program on awareness and practices regarding menstrual hygiene among adolescents’ girls. Methods: This was a quasi-experimental study carried out at high school, named Government Girls High School Kahna Nau, Tehsil Model Town Lahore. Data were collected from overall 110 adolescents’ girls were studying at Government Girls High School Kahna Nau Tehsil Model Town Lahore about awareness and practices regarding menstrual hygiene. Data were collected using awareness and practices questionnaire regarding menstrual hygiene. Pre and Post-interventional awareness and practices was assessed using the same questionnaire. SPSS 25.0 was used for data analysis. Results: Overall 73.6% of the adolescent’s girls had reported poor pre-interventional awareness, which enhanced to excellent awareness (100%) after interventions. Similarly, 79.1% of the adolescent girls were reported unsafe practices in pre-intervention phase while 94.5% of the adolescent girls reported safe practices after interventions. There was significant difference among pre and post-interventional awareness (P<0.001) and practices (P<0.001) of adolescent girls regarding menstrual hygiene. Conclusions: Adolescent girls reported poor awareness and unsafe practices regarding menstrual hygiene. The findings of the study showed that educational interventions are the key for enhancing the awareness and practices of adolescents regarding menstrual hygiene.