HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution

Kuo, Feng-Chih; Neville, Matt J.; Sabaratnam, Rugivan; Wesolowska-Andersen, Agata; Phillips, Daniel J.; Wittemans, Laura B. L.; Dam, Anke; Loh, Nellie Y.; Todorčević, Marijana; Denton, Nathan; Kentistou, Katherine A.; Joshi, Peter K.; Christodoulides, Constantinos; Langenberg, Claudia; Collas, Philippe; Karpe, Fredrik; Pinnick, Katherine E.

doi:10.1016/j.celrep.2022.111136

Cited by 25 publications

(15 citation statements)

References 104 publications

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“…The result preliminarily indicated that HOTAIR might participate in the regulation of the NF-κB signaling pathway by regulating the expression of IκBα. There has been reports that HOTAIR could recruit the PRC2 complex to regulate the expression of target genes ( 15 , 25 , 31 , 32 ). In order to study whether HOTAIR could also interact with PRC2 in BCSCs, we carried out RNA pulldown and found that the components enhancer of zeste homolog 2 (EZH2) and suppressor of zeste 12 (SUZ12) of the PRC2 could be detected ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Long noncoding RNA HOTAIR regulates the stemness of breast cancer cells via activation of the NF-κB signaling pathway

Wang¹,

Liu²,

Li³

et al. 2022

Journal of Biological Chemistry

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Section: Resultsmentioning

confidence: 99%

Long noncoding RNA HOTAIR regulates the stemness of breast cancer cells via activation of the NF-κB signaling pathway

Wang¹,

Liu²,

Li³

et al. 2022

Journal of Biological Chemistry

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“…Here, we showed that HOTAIR expression in SF is decreased after stimulation by local inflammatory factors, which could explain this decreased expression in RA SF. Other recent studies have shown that HOTAIR expression could be regulated by various factors in the local microenvironment, such as hypoxia (43), hormons (24) or inflammatory factors (44).…”

Section: Discussionmentioning

confidence: 99%

“…Beyond the joint, HOTAIR appears to be involved in the site-specific regulation of inflammation in other organs. Thus, it has recently been identified as a major regulator of region-specific development of adipose tissue, which is associated with site-specific metabolic complications, with exclusive expression in gluteofemoral subcutaneous adipose tissue (24,55). In addition, HOTAIR is mostly expressed in distal dermal fibroblasts (18).…”

Section: Discussionmentioning

confidence: 99%

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The long-non coding RNA HOTAIR as site specific regulator of inflammation in chronic arthritis

Elhaï¹,

Micheroli

Houtman

et al. 2023

Preprint

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Most forms of arthritis, have a distinctive topographical pattern of joint involvement. Beyond these differences among diseases, there are also differences in phenotype and response to treatment between joints of the same type of arthritis, suggesting that molecular mechanisms may differ depending on joint location. Here we show that there are joint-specific molecular and tissue changes in the synovium and in local stromal cells (synovial fibroblasts;SF). The long non-coding RNA HOTAIR, expressed only in lower extremities SF, regulates much of this site-specific gene expression in SF. Downregulation of HOTAIR after TNF stimulation regulated relevant inflammatory pathways by epigenetic and transcriptional mechanisms and modified the migratory function of SF, decreased SF-mediated osteoclastogenesis, and increased the attraction of B cells by SF. Since site-specific expression of HOTAIR was also measured in the skin, spine and gastrointestinal tract, we propose HOTAIR as important epigenetic factor that modulates site-specific phenotypes of chronic inflammation.

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“…Nqo1-AS1 upregulates Nqo1 expression by binding to the Nqo1 3 UTR and increasing Nqo1 mRNA stability, thereby attenuating cigarette smokeinduced oxidative stress [4]. Although many studies have been conducted on antisense lncRNAs [5], the role of lncRNAs in porcine adipogenesis remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

LncPLAAT3-AS Regulates PLAAT3-Mediated Adipocyte Differentiation and Lipogenesis in Pigs through miR-503-5p

Wang

Chai

Wang

et al. 2023

Genes

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Animal fat deposition has a significant impact on meat flavor and texture. However, the molecular mechanisms of fat deposition are not well understood. LncPLAAT3-AS is a naturally occurring transcript that is abundant in porcine adipose tissue. Here, we focus on the regulatory role of lncPLAAT3-AS in promoting preadipocyte proliferation and adipocyte differentiation. By overexpressing or repressing lncPLAAT3 expression, we found that lncPLAAT3-AS promoted the transcription of its host gene PLAAT3, a regulator of adipocyte differentiation. In addition, we predicted the region of lncPLAAT3-AS that binds to miR-503-5p and showed by dual luciferase assay that lncPLAAT3-AS acts as a sponge to absorb miR-503-5p. Interestingly, miR-503-5p also targets and represses PLAAT3 expression and helps regulate porcine preadipocyte proliferation and differentiation. Taken together, these results show that lncPLAAT3-AS upregulates PLAAT3 expression by absorbing miR-503-5p, suggesting a potential regulatory mechanism based on competing endogenous RNAs. Finally, we explored lncPLAAT3-AS and PLAAT3 expression in adipose tissue and found that both molecules were expressed at significantly higher levels in fatty pig breeds compared to lean pig breeds. In summary, we identified the mechanism by which lncPLAAT3-AS regulates porcine preadipocyte proliferation and differentiation, contributing to our understanding of the molecular mechanisms of lipid deposition in pigs.

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HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution

Cited by 25 publications

References 104 publications

Long noncoding RNA HOTAIR regulates the stemness of breast cancer cells via activation of the NF-κB signaling pathway

Long noncoding RNA HOTAIR regulates the stemness of breast cancer cells via activation of the NF-κB signaling pathway

The long-non coding RNA HOTAIR as site specific regulator of inflammation in chronic arthritis

LncPLAAT3-AS Regulates PLAAT3-Mediated Adipocyte Differentiation and Lipogenesis in Pigs through miR-503-5p

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