Housekeeping gene variability in normal and cancerous colorectal, pancreatic, esophageal, gastric and hepatic tissues

Rubie, Claudia; Kempf, Katja; Hans, Joachim; Su, Tiefen; Tilton, Bettina; Georg, Thomas; Brittner, Brigitte; Ludwig, Bjoern; Schilling, Martin K.

doi:10.1016/j.mcp.2004.10.001

Cited by 254 publications

(195 citation statements)

References 31 publications

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“…This difference in housekeeping gene expression between tumor tissues and adjacent normal tissues has been well documented. 28 Three independent tissue blots were immunoblotted with the indicated antibodies, and densitometric analysis CMA and cancer has yet to be established. While this manuscript was under review, a report was published suggesting the role of CMA in tumor growth.…”

Section: Lamp2a Overexpression In Breast Tumors Promotes Cancer Cell mentioning

confidence: 99%

LAMP2A overexpression in breast tumors promotes cancer cell survival via chaperone-mediated autophagy

2012

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Section: Lamp2a Overexpression In Breast Tumors Promotes Cancer Cell mentioning

confidence: 99%

LAMP2A overexpression in breast tumors promotes cancer cell survival via chaperone-mediated autophagy

2012

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“…ACTB was chosen as the endogenous control for expression studies in gastric cancer tissues since it showed the lowest variability across samples in the microarray data and had been identified as the most appropriate housekeeper for this tissue type. 28 GAPDH was used to control expression analyses in cell lines. For expression analyses in fibroblasts from indirect coculture experiments, TaqMan s -PCRs were conducted using the 'RNA UltraSenset One-Step Quantitative RT-PCR System' (Invitrogen).…”

Section: Gene Ontology Analysismentioning

confidence: 99%

THBS4, a novel stromal molecule of diffuse-type gastric adenocarcinomas, identified by transcriptome-wide expression profiling

et al. 2011

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Gastric adenocarcinomas can be divided into two major histological types, the diffuse and intestinal type (Laurén classification). Since they diverge in many clinical and molecular characteristics, it is widely accepted that they represent distinct disease entities that may benefit from different therapeutic approaches. Gene expression profiling studies have identified numerous genes that are differentially expressed between them. However, none of these studies covered the whole transcriptome and the published gene lists reveal little overlap, raising the need for further, more comprehensive analyses. Here, we present the first transcriptome-wide expression profiling study comparing the two types (diffuse n ¼ 19, intestinal n ¼ 24), which identified 41000 genes that are differentially expressed. Among them, thrombospondin 4 (THBS4) showed the strongest correlation to histological type, with vast overexpression in the diffuse type. Quantitative real-time PCR validated this strong overexpression and revealed that intestinal tumors generally lack THBS4 expression. Immunohistochemistry demonstrated THBS4 overexpression on the protein level (n ¼ 10) and localized THBS4 to the stromal aspect. Its expression was primarily observed within the extracellular matrix surrounding the tumor cells, with the highest intensities found in regions of high tumor cell density and invasion. Intestinal tumors and matched non-neoplastic gastric epithelium and stroma did not feature any relevant THBS4 expression in a preliminary selection of analyzed cases (n ¼ 5). Immunohistochemical colocalization and in vitro studies revealed that THBS4 is expressed and secreted by cancer-associated fibroblasts. Furthermore, we show that THBS4 transcription in fibroblasts is stimulated by tumor cells. This study is the first to identify THBS4 as a powerful marker for diffuse-type gastric adenocarcinomas and to provide an initial characterization of its expression in the course of this disease.

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“…Gene expression of IL-8 was analyzed in relation to the levels of the slope matched housekeeping gene Cyclophilin C (CycC) [29] . Since reporting of data obtained from raw CT values falsely represent the variations, we converted the individual CT values to the linear form as follows:…”

Section: Real-time Pcrmentioning

confidence: 99%

Correlation of IL-8 with induction, progression and metastatic potential of colorectal cancer

Rubie

Frick²,

Pfeil³

et al. 2007

WJG

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107

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AIM:To investigate the expression profile of IL-8 in inflammatory and malignant colorectal diseases to evaluate its potential role in the regulation of colorectal cancer (CRC) and the development of colorectal liver metastases (CRLM).METHODS: IL-8 expression was assessed by quantitative real-time PCR (Q-RT-PCR) and the enzyme-linked immunosorbent assay (ELISA) in resected specimens from patients with ulcerative colitis (UC, n = 6) colorectal adenomas (CRA, n = 8), different stages of colorectal cancer (n = 48) as well as synchronous and metachronous CRLM along with their corresponding primary colorectal tumors (n = 16).RESULTS: IL-8 mRNA and protein expression was significantly up-regulated in all pathological colorectal entities investigated compared with the corresponding neighboring tissues. However, in the CRC specimens IL-8 revealed a significantly more pronounced overexpression in relation to the CRA and UC tissues with an average 30-fold IL-8 protein up-regulation in the CRC specimens in comparison to the CRA tissues. Moreover, IL-8 expression revealed a close correlation with tumor grading. Most interestingly, IL-8 up-regulation was most enhanced in synchronous and metachronous CRLM, if compared with the corresponding primary CRC tissues. Herein, an up to 80-fold IL-8 overexpression in individual metachronous metastases compared to normal tumor neighbor tissues was found. CONCLUSION:Our results strongly suggest an association between IL-8 expression, induction and progression of colorectal carcinoma and the development of colorectal liver metastases.

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Housekeeping gene variability in normal and cancerous colorectal, pancreatic, esophageal, gastric and hepatic tissues

Cited by 254 publications

References 31 publications

LAMP2A overexpression in breast tumors promotes cancer cell survival via chaperone-mediated autophagy

LAMP2A overexpression in breast tumors promotes cancer cell survival via chaperone-mediated autophagy

THBS4, a novel stromal molecule of diffuse-type gastric adenocarcinomas, identified by transcriptome-wide expression profiling

Correlation of IL-8 with induction, progression and metastatic potential of colorectal cancer

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