How biomass composition determines protein extractability

Sari, Yessie Widya; Syafitri, Utami Dyah; Sanders, J.P.M.; Bruins, Marieke E.

doi:10.1016/j.indcrop.2015.03.020

Cited by 61 publications

(47 citation statements)

References 62 publications

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“…The chemical composition and structure of cell walls in legume and grass species may affect the protein extraction as pre-treatment techniques that alter the cell wall structure resulted in enhanced protein extraction yields. 25 The increase in aNDF with advancing maturity is probably a result of changes in the composition of the cell wall with maturity. 26 Grasses contain more NDF compared with legumes, which is mostly due to differences in the NDF contents between grass and legume leaves.…”

Section: Dry Matter Yield and Chemical Compositionmentioning

confidence: 99%

Dry matter yield, chemical composition and estimated extractable protein of legume and grass species during the spring growth

et al. 2017

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White clover could be a good candidate for protein production purpose in a biorefinery due to its high extractable protein content per kg DM. In order to maximise the protein production capacity, harvest should take place during early growth due to a decline in protein extractability with maturity. The final economy of the concept will depend on the value of the fibre after extraction of the protein. © 2017 Society of Chemical Industry.

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Section: Dry Matter Yield and Chemical Compositionmentioning

confidence: 99%

Dry matter yield, chemical composition and estimated extractable protein of legume and grass species during the spring growth

et al. 2017

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“…The yield of protein extracted from the leaf can differ as a result of different plant materials used and the extraction conditions (Bals et al, 2012;Sari, Syafitri, Sanders, & Bruins, 2015). The pre-treatment techniques that disrupt the cell wall structure resulted in a marked increase in the extracted protein yield (Oldfield, Taylor, & Singh, 2005).…”

Section: True Protein Extractabilitymentioning

confidence: 99%

Estimation of extractable protein in botanical fractions of legume and grass species

Solati

Jørgensen

Eriksen

et al. 2017

Grass and Forage Science

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With a globally strong interest in bio‐based products such as fuels and chemicals, a feasible source of protein for the industry with positive economic impacts could be from leaves. However, more knowledge is needed on how to improve the content of extractable protein. Grasses and legumes have a high content of protein with a favourable amino acid composition. The extractable true protein was estimated at two harvest dates in leaf and stem of the legume species white clover (Trifolium repens L.), red clover (Trifolium pratense L.) and lucerne (Medicago sativa L.) and the grass species perennial ryegrass (Lolium perenne L.) and tall fescue (Festuca arundinacea L.) using the Cornell Net Carbohydrate and Protein System. Extractable true protein was more concentrated in the leaf than in the stem of all the species. Easily extractable true protein in the leaf constituted 63%–68% and 54% of crude protein in legumes and grasses respectively. If the cell wall‐bound true protein in the leaf can be extracted, concentration of extractable true protein may increase by 7%–14% and 21%–26% of crude protein in legume and grass respectively. Legume leaf could be superior to grass leaf for protein production in a biorefinery due to higher extractable true protein both on a mass basis and per hectare. In white clover leaf, a significant decline in concentration of extractable true protein with maturity was simultaneous with a substantial increase in extractable true protein per hectare.

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“…The yield of extracted protein is one of the most critical parameters in producing plant protein isolates, along with protein quality and concentration in the product. Protein extractability depends to a high degree on the type of protein, its native or denatured state, and the matrix from which the protein is extracted . BSG contains prolamin aggregates and glutelins that are difficult to solubilize: accordingly, only 41% yields of extracted protein have been reported from BSG without the use of detergents or protein‐digesting enzymes .…”

Section: Introductionmentioning

confidence: 99%

High Yield Protein Extraction from Brewer's Spent Grain with Novel Carboxylate Salt - Urea Aqueous Deep Eutectic Solvents

et al. 2017

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The sustainability of the global protein production is seriously challenged, why upgrading protein from new or under‐utilized sources such as brewer's spent grain (BSG) is desirable. In this article a new method for high‐yield protein extraction from BSG with deep eutectic solvents (DESs) is reported. Novel carboxylate salt ‐ urea DESs are described and protein extraction from BSG using 6 of these mixtures is compared. The extraction with 90 wt% NaAcO : urea (molar ratio 1:2) was compared to choline chloride : urea (1:2), of which the first was found to be a more suitable protein extractant with up to 79% extraction yields from BSG. Based on microscopy, it is proposed that the high extraction yield from BSG with 90 wt% NaAcO : urea (1:2) was due to dissolution of proteins insoluble by nature or denatured during the brewing process. The production of protein concentrates with a protein content of >50 wt% was demonstrated and the fractions were characterized in detail.

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How biomass composition determines protein extractability

Cited by 61 publications

References 62 publications

Dry matter yield, chemical composition and estimated extractable protein of legume and grass species during the spring growth

Dry matter yield, chemical composition and estimated extractable protein of legume and grass species during the spring growth

Estimation of extractable protein in botanical fractions of legume and grass species

High Yield Protein Extraction from Brewer's Spent Grain with Novel Carboxylate Salt - Urea Aqueous Deep Eutectic Solvents

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