A comparative approach of hydrological variables in order to clarify the abundance and diversity of the zooplankton community was investigated on a spring cruise in the Northern and Southern coastal waters of Sfax coupled with water-quality. Environmental and zooplankton data were taken across the tidal front TF, covering well-mixed MZ and frontal interface FI waters. Zooplankton abundance, structure, specific composition and life histories were analyzed in relation to depth, temperature, salinity, orthophosphate, major and trace elements and chlorophyll-a. Zooplankton-specific composition did not vary across the different zones but, due to differences in relative abundance in Southern and Northern part. Three main copepod assemblages were revealed and characterized by the distribution of physico-chemical variables and chl-a. A strong divergence of zooplankton group was deduced through both multivariate analyses (NMDS, PCA and CCA), reflecting changes in zooplankton between the three typical zones. Results suggest that environmental parameters related differential responses of zooplankton should be considered when zooplankton monitoring is performed to assess the effect of climate forcing and pollution in coastal environments. Data on the distribution of copepod assemblages in the sfax coastline are scarce makes our contribution useful for further global effect of climate forcing and pollution in coastal environments in the Mediterranean Sea.