How has dental pulp stem cells isolation been conducted? A scoping review

Ferrúa, Camila Perelló; Centeno, Eduarda Gervini Zampieri; Rosa, Laísa Camerini da; Amaral, Cainá Corrêa do; Severo, Rafaely Ferreira; Sarkis‐Onofre, Rafael; Nascimento, Gustavo G.; Cordenonzi, Gabriele; Krolow, Rachel; Demarco, Flávio Fernando; Nedel, Fernanda

doi:10.1590/1807-3107bor-2017.vol31.0087

Cited by 26 publications

(22 citation statements)

References 39 publications

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“…Over 20 different enzymes and their combinations were considered during the DPSC dissociation methods. Collagenase I associated with dispase and collagenase alone were the most frequently used methods [16]. A recent research showed it will enrich more cells with CD146+, which were considered as the mainly DPSCs marker, as well as osteogenic and chondrogenic ability [14].…”

Section: Isolation Culture and Cryopreservation Of Dpscsmentioning

confidence: 99%

Recycle the dental fairy’s package: overview of dental pulp stem cells

Yang

Xiao

et al. 2018

Stem Cell Res Ther

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Adult stem cells are excellent cell resource for cell therapy and regenerative medicine. Dental pulp stem cells (DPSCs) have been discovered and well known in various application. Here, we reviewed the history of dental pulp stem cell study and the detail experimental method including isolation, culture, cryopreservation, and the differentiation strategy to different cell lineage. Moreover, we discussed the future potential application of the combination of tissue engineering and of DPSC differentiation. This review will help the new learner to quickly get into the DPSC filed.

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Section: Isolation Culture and Cryopreservation Of Dpscsmentioning

confidence: 99%

Recycle the dental fairy’s package: overview of dental pulp stem cells

Yang

Xiao

et al. 2018

Stem Cell Res Ther

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“…As extracted teeth are typically liquidated as biological waste, it is very convenient to preserve these teeth as a source of DPSC isolation. Dental pulp tissues from impacted or semi-impacted wisdom teeth are most frequently used as a source of DPSCs [ 1 ]. Surgical removal of impacted mandibular third molars should be carried out well before the age of 24 years.…”

Section: Introductionmentioning

confidence: 99%

“…Older patients are at higher risk of postoperative complications [ 2 ]. The second most frequent source of DPSCs involves premolars [ 1 ]. They are mostly extracted during orthodontic treatment of severe tooth crowding, class II malocclusion [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Cryogenic Storage on Human Dental Pulp Stem Cells

Pilbauerova

Schmidt

Soukup

et al. 2021

IJMS

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Dental pulp stem cells (DPSCs) are a type of easily accessible adult mesenchymal stem cell. Due to their ease of access, DPSCs show great promise in regenerative medicine. However, the tooth extractions from which DPSCs can be obtained are usually performed at a period of life when donors would have no therapeutic need of them. For this reason, it is imperative that successful stem cell storage techniques are employed so that these cells remain viable for future use. Any such techniques must result in high post-thaw stem cell recovery without compromising stemness, proliferation, or multipotency. Uncontrolled-rate freezing is not a technically or financially demanding technique compared to expensive and laborious controlled-rate freezing techniques. This study was aimed at observing the effect of uncontrolled-rate freezing on DPSCs stored for 6 and 12 months. Dimethyl sulfoxide at a concentration of 10% was used as a cryoprotective agent. Various features such as shape, proliferation capacity, phenotype, and multipotency were studied after DPSC thawing. The DPSCs did not compromise their stemness, viability, proliferation, or differentiating capabilities, even after one year of cryopreservation at −80 °C. After thawing, they retained their stemness markers and low-level expression of hematopoietic markers. We observed a size reduction in recovery DPSCs after one year of storage. This observation indicates that DPSCs can be successfully used in potential clinical applications, even after a year of uncontrolled cryopreservation.

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“…7,11 Therefore, in order to fully exploit these desirable DPSCs for regenerative purposes, it is imperative that strategies are developed which permit the optimisation of population selection through the selectively screening and isolation of superior quality DPSCs from dental pulp tissues for in vitro expansion, assessment and prudent cell banking; thereby aiding the translational development of more effective DPSC-based therapies for clinical evaluation and application. [12][13][14] However, conventional ex vivo cellular expansion and stem cell/senescence characterisation techniques used to identify superior DPSCs are often expensive, labour-intensive and time-consuming; whilst cellular expansion can also detrimentally alter stem cell characteristics leading to reduced regenerative properties. 11,15 Such issues may be confounded by the requirement for invasive fixation, staining or cell permeabilisation techniques, which damage cellular components during MSC characteristation.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Dental Pulp Stem Cell Regenerative Heterogeneity by Single-Cell Raman Spectroscopy

Alraies

Canetta²,

Waddington

et al. 2019

Tissue Engineering Part C: Methods

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Dental pulp stem cells (DPSCs) are increasingly being recognized as a viable cell source for regenerative medicine. However, significant heterogeneity in their ex vivo expansion capabilities are well-established, which influences their regenerative and therapeutic potentials. As highly proliferative/multi-potent DPSCs are minority sub-populations within dental pulp, the development of non-invasive strategies capable of successfully discriminating between DPSC sub-populations with contrasting proliferative and differentiation capabilities in situ, would be immensely beneficial for the selective screening/isolation of superior quality DPSCs for in vitro assessment and therapy development. Consequently, this study assessed the effectiveness of Single Cell Raman Spectroscopy (SCRM), in distinguishing between DPSC sub-populations with contrasting proliferative and differentiation capabilities isolated from dental pulp tissues. Individual DPSC sub-populations were isolated from human third molars and identified as high or low proliferative and multi-potent or uni-potent, following in vitro expansion and senescence confirmation. High proliferative/multi-potent DPSCs, such as A3 (18PDs and 60PDs) and low proliferative/uni-potent DPSCs, including A1 and B1 (8PDs and 7PDs respectively), were analysed using an iHR550 Raman Spectrometer, equipped with a CCD camera and Eclipse Ti-U Inverted Microscope. Single cell spectra were acquired for 20 cells in each sub-population (10 spectra per nuclear and 10 spectra per cytoplasmic/membrane regions in each cell analysed), over 500-2100 cm-1. Spectra and peak assignments were obtained, followed by PCA and multivariate statistical analysis. Although DPSC spectra contained typical Raman peaks for nucleic acids, proteins and lipids, the spectral intensities of high proliferative/multi-potent DPSCs, A3 (18PDs), were higher than A3 (60PDs), A1 (8PDs) and B1 (7PDs), reflecting significantly elevated DNA (729 cm-1) and protein (1111, 1167, 1245 and 1680 cm-1) contents overall. PCA and multivariate analysis revealed significant variations in scatter plots and Raman signatures, with distinct fingerprints for high proliferative/multi-potent DPSCs, A3 at 18PDs and 60PDs; versus the similar overlapping profiles for low proliferative/uni-potent DPSCs, A1 3 (8PDs) and B1 (7PDs). This study confirms that SCRM successfully discriminates between DPSC sub-populations with contrasting proliferative and differentiation capabilities, advocating its further assessment as a viable technique for the selective non-invasive screening, identification and isolation of high proliferative/multi-potent DPSCs from dental pulp tissues for regenerative medicine applications. Impact Statement This study is the first to investigate and confirm the effectiveness of Single Cell Raman Spectroscopy (SCRM), in its ability to discriminate between DPSCs with contrasting proliferative and differentiation capabilities. The findings show that SCRM can rapidly and noninvasively distinguish and identify DPSC sub-populations in vit...

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How has dental pulp stem cells isolation been conducted? A scoping review

Cited by 26 publications

References 39 publications

Recycle the dental fairy’s package: overview of dental pulp stem cells

Recycle the dental fairy’s package: overview of dental pulp stem cells

The Effects of Cryogenic Storage on Human Dental Pulp Stem Cells

Discrimination of Dental Pulp Stem Cell Regenerative Heterogeneity by Single-Cell Raman Spectroscopy

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