The properties of acceleration fluctuations in isotropic turbulence are studied in direct numerical simulations ͑DNS͒ by decomposing the acceleration as the sum of local and convective contributions ͑a L ϭץu/ץt and a C ϭu•ٌu͒, or alternatively as the sum of irrotational and solenoidal contributions ͓a I ϭϪٌ( p/) and a S ϭٌ 2 u͔. The main emphasis is on the nature of the mutual cancellation between a L and a C which must occur in order for the acceleration ͑a͒ to be small as predicted by the ''random Taylor hypothesis'' ͓Tennekes, J. Fluid Mech. 67, 561 ͑1975͔͒ of small eddies in turbulent flow being passively ''swept'' past a stationary Eulerian observer. Results at Taylor-scale Reynolds number up to 240 show that the random-Taylor scenario ͗a 2 ͘Ӷ͗a C 2 ͘ Ϸ͗a L 2 ͘, accompanied by strong antialignment between the vectors a L and a C , is indeed increasingly valid at higher Reynolds number. Mutual cancellation between a L and a C also leads to the solenoidal part of a being small compared to its irrotational part. Results for spectra in wave number space indicate that, at a given Reynolds number, the random Taylor hypothesis has greater validity at decreasing scale sizes. Finally, comparisons with DNS data in Gaussian random fields show that the mutual cancellation between a L and a C is essentially a kinematic effect, although the Reynolds number trends are made stronger by the dynamics implied in the Navier-Stokes equations.