How much solute is needed to inhibit the fluid to gel membrane phase transition at low hydration?

Lenné, Thomas; Bryant, Gary; Holcomb, Roland; Koster, Karen L.

doi:10.1016/j.bbamem.2007.01.008

Cited by 32 publications

(54 citation statements)

References 19 publications

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“…Sucrose can also avoid the rise in membrane phase transition temperature during dehydration (Bryant et al 2001;Koster 2001). Recently, Lenné et al (2007) reported that the effect of sugars on membrane phase transition is maximized at a particular sugar concentration. Sucrose content in M. polymorpha cultured cells made up 14% of DW and was the same as that in dehydrated leaves of resurrection plants reported to date (Scott 2000).…”

Section: Discussionmentioning

confidence: 99%

Development of desiccation tolerance and vitrification by preculture treatment in suspension-cultured cells of the liverwort Marchantia polymorpha

Hatanaka

Sugawara

2010

Planta

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Some cultured plant cells are able to acquire tolerance to various stresses when they are cultured under suitably controlled conditions. Induction of a high level of desiccation tolerance in suspension-cultured cells of the liverwort Marchantia polymorpha was examined for studying the mechanisms of desiccation tolerance and vitrification at the cellular level. Desiccation tolerance level of cells was very low and the survival rate was less than 10% after exposure to drying below 0.1 g H(2)O g(-1) dry weight (DW). Preculture treatment in 0.5 M sucrose medium was the most effective method for inducing a high level of desiccation tolerance in cells and the survival rate was 87% even after being desiccated to below 0.1 g H(2)O g(-1) DW. Preculture treatment caused alteration of cell structures and accumulation of a large amount of sucrose and newly synthesized proteins in cells. Abundant sucrose and preculture-induced proteins were necessary for full development of desiccation tolerance in the cells. When water content decreased to below 0.1 g H(2)O g(-1) DW, desiccation-tolerant cells that had been precultured were vitrified above 0 degrees C and maintained stable viability. We have succeeded in the induction of desiccation tolerance that allows formation of intracellular glass with cell viability at ambient temperatures by controlling culture conditions, and our results suggest that suspension-cultured cells of M. polymorpha are useful for studying cellular mechanisms for the development of desiccation tolerance and the stabilization of vitrified cells.

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Section: Discussionmentioning

confidence: 99%

Development of desiccation tolerance and vitrification by preculture treatment in suspension-cultured cells of the liverwort Marchantia polymorpha

Hatanaka

Sugawara

2010

Planta

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“…Phase transitions which affect the partitioning between the intra-and extra-cellular compartments (e.g. lamellar to hexagonal phase transition 9 ), or change the dynamic nature of the hydrophobic domain through more rigid lipid chain packing (lamellar fluid to gel phase transition 10 ) are found to be inhibited by sugar molecules in both model systems and biological cells. [11][12] Several molecular mechanisms have been proposed to explain the stabilization of the fluid lamellar phase and the protective mechanism of sugars.…”

Section: Introductionmentioning

confidence: 99%

Direct Comparison of Disaccharide Interaction with Lipid Membranes at Reduced Hydrations

et al. 2015

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Abstract:Understanding sugar-lipid interactions during desiccation and freezing is an important step in the elucidation of cryo-and anhydro-protection mechanisms. We determine sucrose, trehalose and water concentration distributions in intra-bilayer volumes between opposing dioleoylphosphatidylcholine bilayers over a range of reduced hydrations and sugar concentrations. Stacked lipid bilayers at reduced hydration provide a suitable system to mimic environmental dehydration effects, as well as a suitable system for direct probing of sugar locations by neutron membrane diffraction. Sugar distributions show that sucrose and trehalose both behave as typical uncharged solutes, largely excluded from the lipid bilayers regardless of sugar identity, and with no correlation between sugar distribution and the lipid headgroup position as the hydration is changed. These results are discussed in terms of current opinions about cryo-and anhydro-protection mechanisms.2

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“…In order to shed some light on this problem we have recently reported results using small angle neutron scattering (SANS)-contrast variation (Lenné et al, 2006), small and wide angle X-ray scattering SAXS/WAXS (Lenné et al, 2009) and differential scanning calorimetry (DSC) (Lenné et al, 2007). This work has made significant progress in clarifying and quantifying the effects of sugars on the gel-fluid transition.…”

Section: Introductionmentioning

confidence: 99%

Kinetics of the lamellar gel–fluid transition in phosphatidylcholine membranes in the presence of sugars

Lenné

Garvey

Koster

et al. 2010

Chemistry and Physics of Lipids

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Phase diagrams are presented for dipalmitoylphosphatidylcholine (DPPC) in the presence of sugars (sucrose) over a wide range of relative humidities (RHs). The phase information presented here, determined by small angle X-ray scattering (SAXS), is shown to be consistent with previous results achieved by differential scanning calorimetry (DSC). Both techniques show a significant effect of sucrose concentration on the phase behaviour of this phospholipid bilayer. An experimental investigation into the effect of sugars on the kinetic behaviour of the gel to fluid transition is also presented showing that increasing the sugar content appears to slightly increase the rate at which the transition occurs.

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How much solute is needed to inhibit the fluid to gel membrane phase transition at low hydration?

Cited by 32 publications

References 19 publications

Development of desiccation tolerance and vitrification by preculture treatment in suspension-cultured cells of the liverwort Marchantia polymorpha

Development of desiccation tolerance and vitrification by preculture treatment in suspension-cultured cells of the liverwort Marchantia polymorpha

Direct Comparison of Disaccharide Interaction with Lipid Membranes at Reduced Hydrations

Kinetics of the lamellar gel–fluid transition in phosphatidylcholine membranes in the presence of sugars

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