2015
DOI: 10.1074/jbc.m114.619205
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How pH Modulates the Dimer-Decamer Interconversion of 2-Cys Peroxiredoxins from the Prx1 Subfamily

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Cited by 47 publications
(51 citation statements)
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“…Note that the hydrodynamic radius (R H ) of the protein decreases upon the addition of EDTA in samples pretreated with CaCl 2 . conformation of region I required for decamer stabilization (9). As expected, we found a site in which Ca 2ϩ could be coordinated by residues from both interfacing subunits, assuming a distorted trigonal bipyramidal geometry according to molecu-lar dynamics simulations (Fig.…”
Section: Structural Basis For the Cation-dependent Mechanism Of Decamsupporting
confidence: 78%
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“…Note that the hydrodynamic radius (R H ) of the protein decreases upon the addition of EDTA in samples pretreated with CaCl 2 . conformation of region I required for decamer stabilization (9). As expected, we found a site in which Ca 2ϩ could be coordinated by residues from both interfacing subunits, assuming a distorted trigonal bipyramidal geometry according to molecu-lar dynamics simulations (Fig.…”
Section: Structural Basis For the Cation-dependent Mechanism Of Decamsupporting
confidence: 78%
“…Like other members of the AhpC/Prx1 subfamily (30), the LbPrx1m decamer is formed when five dimers bind to each other via the A-type interface (9). Because the formation of this interface depends on conformational changes in region I (residues 75-79) preceding the C p -loop (residues 80 -84) (9), we hypothesized that the decamer-stabilizing effect of Ca 2ϩ /Mg 2ϩ might be related to their binding to the A-type interface and the concomitant stabilization of region I in a conformation that favors the decameric assembly.…”
Section: Structural Basis For the Cation-dependent Mechanism Of Decammentioning
confidence: 99%
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“…However, hyperoxidized Prx3 with one disulfide and one hyperoxidized active site was dimeric (Figure 7B). Lowering the pH to 7 stabilizes the decameric structure of Prx2 [27,29]. This pH effect was also observed with Prx3, with the disulfide and the hyperoxidized dimers associating to dodecamers at pH 7 (result not shown).…”
Section: Resultsmentioning
confidence: 83%