How to control the size and morphology of apatite nanocrystals in bone

Xie, Baoquan; Nancollas, G.H.

doi:10.1073/pnas.1017493108

Cited by 117 publications

(115 citation statements)

References 18 publications

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“…One possible reason is that they grow via an OCP transition phase, in which crystals are plate-shaped (Weiner and Wagner 1998). Another explanation involves the presence of citrates, which strongly bound to (10Ī0) surface of biological apatite because of space matching (Hu et al 2010;Xie and Nancollas 2010). Therefore, the crystal growth in the [10Ī0] direction becomes inhibited, while the citrate effect on other crystal surfaces of biological apatite appears Fig.…”

Section: Bonementioning

confidence: 99%

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Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Dorozhkin¹

2017

Morphologie

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Section: Bonementioning

confidence: 99%

“…13 a A schematic to be very small owing to poor space matching. Thus, after crystal growth, the (10Ī0) crystal face becomes predominant resulting in plate-like morphology of biological apatite (Xie and Nancollas 2010).…”

Section: Bonementioning

confidence: 99%

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Dorozhkin¹

2017

Morphologie

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“…Inspired by the critical physiological role of citric acid on bone apatite nanocrystal size control and stabilization [24,25], we have envisaged the citrate-assisted growth of Hap particles as a promising synthesis technique in terms of particle morphology control. Considering the dynamic nature of nanoparticle properties, special attention was given to the particles' characterization.…”

Section: Introductionmentioning

confidence: 99%

“…This chemical entity appears to have a critical role in crystal thickening and stabilization of the apatite nanocrystals through its carboxylic groups [7,24,25]. WCS and HS nanoparticles presented different morphology and crystallinity features which reflect the temperature and pressure conditions that distinguish the two synthesis methods.…”

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Relevance of the sterilization-induced effects on the properties of different hydroxyapatite nanoparticles and assessment of the osteoblastic cell response

Santos

Gomes

Duarte

et al. 2012

J. R. Soc. Interface.

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Hydroxyapatite (Hap) is a calcium phosphate with a chemical formula that closely resembles that of the mineral constituents found in hard tissues, thereby explaining its natural biocompatibility and wide biomedical use. Nanostructured Hap materials appear to present a good performance in bone tissue applications because of their ability to mimic the dimensions of bone components. However, bone cell response to individual nanoparticles and/or nanoparticle aggregates lost from these materials is largely unknown and shows great variability. This work addresses the preparation and characterization of two different Hap nanoparticles and their interaction with osteoblastic cells. Hap particles were produced by a wet chemical synthesis (WCS) at 378C and by hydrothermal synthesis (HS) at 1808C. As the ultimate in vivo applications require a sterilization step, the synthesized particles were characterized 'as prepared' and after sterilization (autoclaving, 1208C, 20 min). WCS and HS particles differ in their morphological (size and shape) and physicochemical properties. The sterilization modified markedly the shape, size and aggregation state of WCS nanoparticles. Both particles were readily internalized by osteoblastic cells by endocytosis, and showed a low intracellular dissolution rate. Concentrations of WCS and HS particles less than 500 mg ml 21 did not affect cell proliferation, F-actin cytoskeleton organization and apoptosis rate and increased the gene expression of alkaline phosphatase and BMP-2. The two particles presented some differences in the elicited cell response. In conclusion, WCS and HS particles might exhibit an interesting profile for bone tissue applications. Results suggest the relevance of a proper particle characterization, and the interest of an individual nanoparticle targeted research.

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Biological and Medical Significance of Nanodimensional and Nanocrystalline Calcium Orthophosphates

Dorozhkin¹

2012

Biomedical Materials and Diagnostic Devices

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How to control the size and morphology of apatite nanocrystals in bone

Cited by 117 publications

References 18 publications

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Relevance of the sterilization-induced effects on the properties of different hydroxyapatite nanoparticles and assessment of the osteoblastic cell response

Biological and Medical Significance of Nanodimensional and Nanocrystalline Calcium Orthophosphates

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