Hox-dependent coordination of mouse cardiac progenitor cell patterning and differentiation

Stefanovic, Sonia; Laforest, Brigitte; Desvignes, Jean-Pierre; Lescroart, Fabienne; Argiro, Laurent; Maurel-Zaffran, Corinne; Salgado, David; Plaindoux, Elise; Bono, Christopher De; Pažur, Kristijan; Théveniau-Ruissy, Magali; Béroud, Christophe; Pucéat, Michel; Gavalas, Anthony; Kelly, Robert G.; Zaffran, Stéphane

doi:10.7554/elife.55124

Cited by 48 publications

(61 citation statements)

References 90 publications

(160 reference statements)

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“…Initial studies on the contribution of the SHF to the developing heart mainly focused on its importance for the expansion of the arterial pole, showing that the anterior SHF (aSHF) primarily contributes to the outflow tract, right ventricle, and ventricular septum [ 9 , 12 , 13 ]. Subsequent studies demonstrated that the posterior SHF (pSHF) plays a role in the development of the venous pole by contributing to the DMP and its derivatives [ 4 , 14 ], the pAS [ 15 ], and to the atrial myocardium ( Figure 1 ), although the extent to which the pSHF contributes to the atrial myocardial tissues is still a matter of debate [ 16 ].…”

Section: Early Heart Developmentmentioning

confidence: 99%

The Mesenchymal Cap of the Atrial Septum and Atrial and Atrioventricular Septation

Deepe

Fitzgerald

Wolters

et al. 2020

JCDD

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In this publication, dedicated to Professor Robert H. Anderson and his contributions to the field of cardiac development, anatomy, and congenital heart disease, we will review some of our earlier collaborative studies. The focus of this paper is on our work on the development of the atrioventricular mesenchymal complex, studies in which Professor Anderson has played a significant role. We will revisit a number of events relevant to atrial and atrioventricular septation and present new data on the development of the mesenchymal cap of the atrial septum, a component of the atrioventricular mesenchymal complex which, thus far, has received only moderate attention.

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Section: Early Heart Developmentmentioning

confidence: 99%

The Mesenchymal Cap of the Atrial Septum and Atrial and Atrioventricular Septation

Deepe

Fitzgerald

Wolters

et al. 2020

JCDD

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“…Perturbation of the SHF during elongation of the forming heart results in a spectrum of OFT defects associated with early embryonic lethality [ 11 , 12 , 13 ]. Lineage tracing analysis in mice has revealed that the SHF is sub-divided into distinct anterior and posterior regions (aSHF and pSHF) [ 14 , 15 ] ( Figure 1 ). Indeed, aSHF progenitors engender the right ventricular and proximal OFT myocardium, while progenitor cells located in the pSHF contribute to the formation of the atrial and atrioventricular septation through development of the dorsal mesenchymal protrusion (DMP) that forms the muscular base of the primary atrial septum [ 16 ].…”

Section: Embryonic Origins Of the Oftmentioning

confidence: 99%

“…Hoxb1 plays a key role in patterning those cardiac progenitor cells that contribute to both cardiac poles [ 15 ]. Progenitor cells expressing Hoxb1 alone or concomitantly with Hoxa1 contribute to the proximal dorsal OFT or distal OFT, respectively ( Figure 1 ).…”

Section: Hox Transcription Factorsmentioning

confidence: 99%

Outflow Tract Formation—Embryonic Origins of Conotruncal Congenital Heart Disease

Stefanovic

Etchevers

Zaffran

2021

JCDD

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Anomalies in the cardiac outflow tract (OFT) are among the most frequent congenital heart defects (CHDs). During embryogenesis, the cardiac OFT is a dynamic structure at the arterial pole of the heart. Heart tube elongation occurs by addition of cells from pharyngeal, splanchnic mesoderm to both ends. These progenitor cells, termed the second heart field (SHF), were first identified twenty years ago as essential to the growth of the forming heart tube and major contributors to the OFT. Perturbation of SHF development results in common forms of CHDs, including anomalies of the great arteries. OFT development also depends on paracrine interactions between multiple cell types, including myocardial, endocardial and neural crest lineages. In this publication, dedicated to Professor Andriana Gittenberger-De Groot and her contributions to the field of cardiac development and CHDs, we review some of her pioneering studies of OFT development with particular interest in the diverse origins of the many cell types that contribute to the OFT. We also discuss the clinical implications of selected key findings for our understanding of the etiology of CHDs and particularly OFT malformations.

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“…1e). Clusters C1 and C18 contains pSHF populations as identified by expression of Hoxb1 5 , Tbx5, Foxf1, and Wnt2 25,26 (Fig. 1f and Extended Data Fig.…”

Section: Identification Of Common Progenitor Cells In the Cpmmentioning

confidence: 99%

“…The pharyngeal apparatus consists of individual bulges of cells termed arches that form in a rostral to caudal manner from mouse embryonic days (E)8-10. 5. The cellular and molecular mechanisms of how CPM cells in the pharyngeal apparatus both are maintained in a progenitor state and are allocated to form the heart and BrMs in mammals, are unknown.…”

Section: Introductionmentioning

confidence: 99%

Single cell multi-omic analysis identifies aTbx1-dependent multilineage primed population in the murine cardiopharyngeal mesoderm

Nomaru

Liu

Bono

et al. 2020

Preprint

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The poles of the heart and branchiomeric muscles of the face and neck are formed from the cardiopharyngeal mesoderm (CPM) within the pharyngeal apparatus. The formation of the cardiac outflow tract and branchiomeric muscles are disrupted in patients with 22q11.2 deletion syndrome (22q11.2DS), due to haploinsufficiency of TBX1, encoding a T-box transcription factor. Here, using single cell RNA-sequencing, we identified a multilineage primed population (MLP) within the CPM, marked by the Tbx1 lineage, which has bipotent properties to form cardiac and skeletal muscle cells. The MLPs are localized within the nascent mesoderm of the caudal lateral pharyngeal apparatus and provide a continuous source of progenitors that undergo TBX1-dependent progression towards maturation. Tbx1 also regulates the balance between MLP maintenance and maturation while restricting ectopic non-mesodermal gene expression. We further show that TBX1 confers this balance by direct regulation of MLP enriched genes and downstream pathways, partly through altering chromatin accessibility. Our study thus uncovers a new cell population and reveals novel mechanisms by which Tbx1 directs the development of the pharyngeal apparatus, which is profoundly altered in 22q11.2DS.

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Hox-dependent coordination of mouse cardiac progenitor cell patterning and differentiation

Cited by 48 publications

References 90 publications

The Mesenchymal Cap of the Atrial Septum and Atrial and Atrioventricular Septation

The Mesenchymal Cap of the Atrial Septum and Atrial and Atrioventricular Septation

Outflow Tract Formation—Embryonic Origins of Conotruncal Congenital Heart Disease

Single cell multi-omic analysis identifies aTbx1-dependent multilineage primed population in the murine cardiopharyngeal mesoderm

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