HOX gene analysis in the osteogenic differentiation of human mesenchymal stem cells

Chae, Song Wha; Jee, Bo Keun; Lee, Joo Yong; Han, Chang Whan; Jeon, Young‐Woo; Lim, Yejee; Lee, Kweon-Haeng; Rha, Hyoung Kyun; Chae, Gue Tae

doi:10.1590/s1415-47572008005000019

Cited by 5 publications

(2 citation statements)

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“…Genes from the HOXC cluster (Homeobox C Cluster) were also reported in muscles where myoblast and myotubes showed differential methylation and that these genes present active promoters and enhancer domains that contain MYOD binding sites [25]. HOXC8 and HOXC11 were reported to be involved in the regulation of osteogenesis [26]. Recently it was suggested by [21] that only a subset of muscles may require HOXC8 protein for full activation of muscle-specific gene expression.…”

Section: Discussionmentioning

confidence: 99%

Gene expression of Hanwoo satellite cell differentiation in longissimus dorsi and semimembranosus

et al. 2019

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Background Korean Hanwoo cattle are known for their high meat quality, especially their high intramuscular fat compared to most other cattle breeds. Different muscles have very different meat quality traits and a study of the myogenic process in satellite cells can help us better understand the genes and pathways that regulate this process and how muscles differentiate. Results Cell cultures of Longissimus dorsi muscle differentiated from myoblast into multinucleated myotubes faster than semimembranosus . Time-series RNA-seq identified a total of 13 differentially expressed genes between the two muscles during their development. These genes seem to be involved in determining muscle lineage development and appear to modulate the expression of myogenic regulatory factors (mainly MYOD and MYF5 ) during differentiation of satellite cells into multinucleate myotubes. Gene ontology enriched terms were consistent with the morphological changes observed in the histology. Most of the over-represented terms and genes expressed during myoblast differentiation were similar regardless of muscle type which indicates a highly conserved myogenic process albeit the rates of differentiation being different. There were more differences in the enriched GO terms during the end of proliferation compared to myoblast differentiation. Conclusions The use of satellite cells from newborn Hanwoo calves appears to be a good model to study embryonic myogenesis in muscle. Our findings provide evidence that the differential expression of HOXB2 , HOXB4 , HOXB9 , HOXC8 , FOXD1 , IGFN1 , ZIC2 , ZIC4 , HOXA11 , HOXC11 , PITX1 , SIM2 and TBX4 genes could be involved in the differentiation of Longissimus dorsi and Semimembranosus muscles. These genes seem to modulate the muscle fate of the satellite cells during myogenesis through a differential expression profile that also controls the expression of some myogenic regulatory factors ( MYOD and MYF5 ). The number of differentially expressed genes across time was unsurprisingly large. In relation to the baseline day 0, there were 631, 155, 175, 519 and 586 DE genes in LD, while in SM we found 204, 0, 615, 761 and 1154 DE genes at days 1, 2, 4, 7 and 14 respectively. Electronic supplementary material The online version of this article (10.1186/s12864-019-5530-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Gene expression of Hanwoo satellite cell differentiation in longissimus dorsi and semimembranosus

et al. 2019

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“…Considerando o envolvimento de HOXD10 na regulação da diferenciação osteoblástica (CHAE et al, 2008), é possível que estas diferenças na caracterização das linhagens estejam relacionadas com o contraste no papel de HOXD10 observado entre elas.…”

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HOXD10 em osteossarcoma pediátrico: análise do seu valor prognóstico e envolvimento nos processos de invasão e metástase

Hakime¹

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À minha orientadora Prof a Dra María Sol Brassesco Annichini, grande responsável por todo meu crescimento profissional desde a iniciação científica. Obrigado por acreditar em mim e me incentivar, mesmo nos momentos mais difíceis.Ao Prof o Dr Luiz Gonzaga Tone por ceder o espaço e infraestrutura para o desenvolvimento de parte dos experimentos do meu projeto.À Faculdade de Medicina de Ribeirão Preto e a Faculdade de Filosofia Ciências e Letras de Ribeirão Preto pela oportunidade e por todo o aprendizado científico.

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Polycomb Protein BMI1 Regulates Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells Downstream of GSK3

Becker¹,

Potapenko²,

Niklaus³

et al. 2016

Stem Cells and Development

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Polycomb proteins such as the B lymphoma Mo-MLV insertion region 1 homolog (BMI1) are essential chromatin factors for the self-renewal and differentiation of embryonic and adult stem cells. BMI1 also plays a critical role in osteogenesis as Bmi1-deficient mice display a skeletal phenotype caused by the exhaustion of the mesenchymal stem cell pool. In this study, we have studied the role of BMI1 in the osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hASCs). BMI1 protein, but not RNA levels, increases during in vitro osteogenic differentiation of hASCs. Overexpression of BMI1 leads to an osteogenic priming of hASCs under nondifferentiating conditions and enhanced osteogenesis upon differentiation, along with increased BMP2 and WNT11 expressions. Conversely, knockdown of BMI1 expression reduces osteogenic differentiation. Furthermore, our studies indicate that during osteogenic differentiation of hASCs, BMI1 is a downstream target of GSK3 signaling. BMI1, therefore, acts as a pro-osteogenic differentiation factor in hASCs and hence it is a promising target for active modulation of hASC-derived osteogenesis.

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HOX gene analysis in the osteogenic differentiation of human mesenchymal stem cells

Cited by 5 publications

References 31 publications

Gene expression of Hanwoo satellite cell differentiation in longissimus dorsi and semimembranosus

Gene expression of Hanwoo satellite cell differentiation in longissimus dorsi and semimembranosus

HOXD10 em osteossarcoma pediátrico: análise do seu valor prognóstico e envolvimento nos processos de invasão e metástase

Polycomb Protein BMI1 Regulates Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells Downstream of GSK3

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