HOXC13-AS accelerates cell proliferation and migration in oral squamous cell carcinoma via miR-378g/HOXC13 axis

Wenlu, Li; Zhu, Qinglin; Zhang, Sanke; Liu, Lei; Zhang, Han; Zhu, Dandan

doi:10.1016/j.oraloncology.2020.104946

Cited by 27 publications

(17 citation statements)

References 18 publications

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“…This lncRNA has been previously identified as a cancer biomarker in cervical cancer 52 , breast cancer 53 , glioma 54 , hepatocellular carcinoma 55 , cholangiocarcinoma 56 , and head and neck squamous cell carcinoma [57][58][59] , which emphasizes the absence of HOXC13-AS expression in most normal tissues except the skin. Additionally, HOXC13-AS reportedly functions as an oncogene promoting proliferation and the epithelial-mesenchymal transition of cancer cells 57 . However, we did not observe a significant effect on normal keratinocyte migration or proliferation (Supplementary Fig.…”

Section: Discussionmentioning

confidence: 96%

Human skin specific long noncoding RNA HOXC13-AS regulates epidermal differentiation by interfering with Golgi-ER retrograde transport

Zhang

Piipponen

Liu

et al. 2022

Preprint

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After a skin injury, keratinocytes switch from a state of homeostasis to one of regeneration leading to the reconstruction of the epidermal barrier. The regulatory mechanism of gene expression underpinning this key switch during human skin wound healing is enigmatic. Long noncoding RNAs (lncRNAs) constitute a new horizon in the understanding of the regulatory programmes encoded in the mammalian genome. By comparing the transcriptome of an acute human wound and skin from the same donor as well as keratinocytes isolated from these paired tissue samples, we generated a list of lncRNAs showing changed expression in keratinocytes during wound repair. Our study focused on HOXC13-AS, a recently evolved human lncRNA specifically expressed in epidermal keratinocytes, and we found that its expression was temporally downregulated during wound healing. In line with its enrichment in suprabasal keratinocytes, HOXC13-AS was found to be increasingly expressed during keratinocyte differentiation, but its expression was reduced by EGFR signalling. After HOXC13-AS knockdown or overexpression in human primary keratinocytes undergoing differentiation induced by cell suspension or calcium treatment and in organotypic epidermis, we found that keratinocyte differentiation was promoted, while the cell inflammatory response was suppressed. Moreover, RNA pull-down assays followed by mass spectrometry and RNA immunoprecipitation analysis revealed that mechanistically HOXC13-AS sequestered the coat complex subunit alpha (COPA) protein and interfered with Golgi-to-endoplasmic reticulum (ER) molecular transport, resulting in ER stress and enhanced keratinocyte differentiation. In summary, we identified HOXC13-AS as a crucial regulator of human epidermal differentiation.

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Section: Discussionmentioning

confidence: 96%

Human skin specific long noncoding RNA HOXC13-AS regulates epidermal differentiation by interfering with Golgi-ER retrograde transport

Zhang

Piipponen

Liu

et al. 2022

Preprint

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“…For example, Liu et al [ 47 ] reported that miR-378g targeted CHI3L1 to regulate the migration, invasion, and EMT of ovarian cancer cells. Li et al [ 48 ] showed that HOXC13 , a target gene of miR-378g, accelerates the malignant behavior of oral squamous cell carcinoma cells. Lin et al [ 37 ] revealed that miR-378g partially enhances the radiosensitivity of NPC cells by targeting SHP-1.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of long non-coding RNA HOTAIR promotes bone marrow mesenchymal stem cell differentiation by sponging microRNA miR-378g that inhibits nicotinamide N-methyltransferase

Wang

Feng

2021

Bioengineered

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Osteoporosis (OP) is associated with a serious social and economic burden. Recent studies have shown that the differential expression of long non-coding RNAs (lncRNAs) is closely related to OP. However, the specific molecular mechanism of HOX transcript antisense intergenic RNA (HOTAIR) remains to be elucidated. The expression of HOTAIR and miR-378g in OP patients was detected using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Bone marrow mesenchymal stem cells (BMSCs) were isolated and cultured, and osteogenic differentiation was induced. Alkaline phosphatase (ALP) and Runt-related transcription factor 2 (RUNX2) were detected by qRT-PCR, ELISA, and Western blotting. Calcium deposition was measured using Alizarin red s (ARS) staining. Molecular interactions between HOTAIR, miR-378g, and nicotinamide N-methyltransferase (NNMT) were detected using a dual-luciferase reporter assay. HOTAIR expression was upregulated and miR-378g level was downregulated in OP patients. HOTAIR expression decreased during the osteogenic differentiation of BMSCs. Silencing HOTAIR or NNMT reduced ALP and RUNX2 levels and promoted calcium deposition. The overexpression of HOTAIR or interference with miR-378g inhibited the osteogenic differentiation of BMSCs. HOTAIR negatively regulates miR-378g by targeting NNMT. HOTAIR is an miR-378g sponge that targets NNMT, inhibits the osteogenic differentiation of BMSCs, and provides a valuable target for the treatment of OP.

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“…HOXC13-AS has been assumed to involve in the invasion and cell proliferation of nasopharyngeal carcinoma via miR-383-3p/ HMGA2 axis (30) and to denote a weak prognosis (31). This lncRNA accelerates the malignancy through the miR-378g/HOXC13 axis, presenting novel thought for facilitating the lncRNA-directed remedy of the cell carcinoma of oral squamous (32). However, the HOXC13-AS expression was insigni cantly up-regulated in the tumor tissue compared to the marginal tissue in the present study.…”

Section: Discussionmentioning

confidence: 99%

HOTAIR, HOXC13, and HOXC10 Are Overexpressed in Gastric Cancer

Habibi

Fakhari

Zamani

et al. 2021

Preprint

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Gastric cancer is concerned as the second leading cause of tumor-associated death worldwide after lung tumors and is specified as one of the most common malignant cancers. Given the increasing importance of HOX genes in gastric cancer research, this study was aimed at exploring the expression profile of HOTAIR, HOXC13, HOXC10, HOXC13-AS, and HOXC‑AS3 in gastric cancer. To achieve this goal, 30 pairs of tumor and normal margin samples were assessed for these genes expression analyses via qRT-PCR. result we found that, the HOTAIR, HOXC13, and HOXC10 expression was significantly increased in cancer tissue samples in comparison with adjacent normal tissue samples, P<0.01. Moreover, there were significant positive correlations between the expressions of genes studied: HOXC‑AS3 and HOXC10 (r=0.52, P<0.003), HOXC13-AS and HOXC13 (r=0.57, P<0.001), HOTAIR and HOXC‑AS3 (r=0.39, P<0.03), HOTAIR and HOXC13-AS (r=0.36, P<0.05). The HOXC13 and HOXC10 expression exhibited a significant relationship with both distant metastasis and perineural invasion (metastasis: P<0.014, r=0.443 and P<0.0041, r=0.51 perineural invasion: P<0.025, r=0.41 and P<0.0017, r=0.55). The HOXC13-AS displayed a significant relationship with the sex factor so that in females the expression of this gene was higher than males (P<0.030, r=0.4). Our findings suggest that the expression of HOXC genes and HOTAIR lncRNA increased through gastric tumor progression and thus they possibly participate in malignant transformation and gastric carcinoma metastasis.

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HOXC13-AS accelerates cell proliferation and migration in oral squamous cell carcinoma via miR-378g/HOXC13 axis

Cited by 27 publications

References 18 publications

Human skin specific long noncoding RNA HOXC13-AS regulates epidermal differentiation by interfering with Golgi-ER retrograde transport

Human skin specific long noncoding RNA HOXC13-AS regulates epidermal differentiation by interfering with Golgi-ER retrograde transport

Knockdown of long non-coding RNA HOTAIR promotes bone marrow mesenchymal stem cell differentiation by sponging microRNA miR-378g that inhibits nicotinamide N-methyltransferase

HOTAIR, HOXC13, and HOXC10 Are Overexpressed in Gastric Cancer

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