HPCE quantification of 5-methyl-2′-deoxycytidine in genomic DNA: Methodological optimization for chestnut and other woody species

Hasbún, Rodrigo; Valledor, Luís; Rodrı́guez, José Luis; Santamaría, M. Estrella; Ríos, Darcy; Sánchez, Manuel Ortigueira; Cañal, María Jesús; Rodrı́guez, Roberto

doi:10.1016/j.plaphy.2008.04.009

Cited by 28 publications

(19 citation statements)

References 21 publications

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“…Global DNA methylation was measured according to Hasbú n et al (2008). In brief, each sample 5 mL of DNA (0.5-2 mg mL À 1 ) was denatured and hydrolyzed by adding 0.25 mL of 10 mM ZnSO 4 and 0.5 mL of nuclease P1 (Sigma-Aldrich S.A.; 200 U/mL in 30 mM C 2 H 3 O 2 Na).…”

Section: Dna Methylation Level Measurementmentioning

confidence: 99%

Variations in DNA methylation, acetylated histone H4, and methylated histone H3 during Pinus radiata needle maturation in relation to the loss of in vitro organogenic capability

Valledor

Meijón

Hasbún

et al. 2010

Journal of Plant Physiology

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Section: Dna Methylation Level Measurementmentioning

confidence: 99%

Variations in DNA methylation, acetylated histone H4, and methylated histone H3 during Pinus radiata needle maturation in relation to the loss of in vitro organogenic capability

Valledor

Meijón

Hasbún

et al. 2010

Journal of Plant Physiology

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“…Genomic DNA extraction was carried out with a plant genomic DNA extraction kit (DNeasy Plant MiniKit, Qiagen) following the instructions of the supplier with some modifications to obtain a RNA-free DNA (Hasbún et al 2008).…”

Section: Dna Extractionmentioning

confidence: 99%

DNA demethylation and decrease on free polyamines is associated with the embryogenic capacity of Pinus nigra Arn. cell culture

Noceda

Salaj

Pérez

et al. 2009

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Embryogenic cell lines initiated from immature zygotic embryos of Pinus nigra Arn. ssp. Austriaca were characterized in terms of macromorphological traits (colour, bipolar structures formation, germination ability) and their embryogenic potential was defined as high, medium or null. Quantification of global genomic DNA methylation revealed the existence of specific DNA methylation levels for the determinated embryogenic potentials. The line considered as effectively embryogenic, i.e., with the ability of develop the whole embryogenic program and producing plants, showed the lowest methylation levels. There was also proved the existence of an inverse relationship between total contents of free PAs and embryogenic potential, being the highest contents of free putrescine and spermidine in the non-embryogenic line and the lowest in the effectively embryogenic one. Relationships among DNA methylation levels, profiles of free individual polyamine contents and embryogenic potentials based on the ability to produce well-formed somatic embryos with effective plant conversion are discussed.

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“…Hydrolyzed solutions were centrifuged for 20 min at 15,000g. Finally samples were analyzed by HPCE (CIA, Waters Chromatography) according to Fraga et al (2002) with the modifications of Hasbún et al (2008).…”

Section: Global Dna Methylation Quantification By High Performance Camentioning

confidence: 99%

Epigenetic characterization of the vegetative and floral stages of azalea buds: Dynamics of DNA methylation and histone H4 acetylation

Meijón

Valledor

Santamaría

et al. 2009

Journal of Plant Physiology

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Floral induction in plants is achieved through a complex genetic network and regulated by multiple environmental and endogenous cues. Epigenetic control is determinative in plants for coordinating the switch to flowering under favorable environmental conditions and achieving reproductive success. Global DNA methylation, whose increase is associated with heterochromatinization-cell differentiation, and histone H4 acetylation, which is linked to euchromatin, were analyzed in vegetative and floral buds of azalea in order to study the involvement of epigenetic mechanisms in the floral development of woody plants. The results showed an increase of DNA methylation in floral buds in contrast to the decrease observed for acetylated H4 (AcH4) levels. In addition, when the distributions of 5-mdC and AcH4 in vegetative and floral buds of azalea were analyzed by immunodetection, opposite patterns in their distribution were revealed and confirmed the existence of different cell types in the shoot apical meristem with varying degrees of differentiation.

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HPCE quantification of 5-methyl-2′-deoxycytidine in genomic DNA: Methodological optimization for chestnut and other woody species

Cited by 28 publications

References 21 publications

Variations in DNA methylation, acetylated histone H4, and methylated histone H3 during Pinus radiata needle maturation in relation to the loss of in vitro organogenic capability

Variations in DNA methylation, acetylated histone H4, and methylated histone H3 during Pinus radiata needle maturation in relation to the loss of in vitro organogenic capability

DNA demethylation and decrease on free polyamines is associated with the embryogenic capacity of Pinus nigra Arn. cell culture

Epigenetic characterization of the vegetative and floral stages of azalea buds: Dynamics of DNA methylation and histone H4 acetylation

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