HPLC Analysis of Amino Acids Content in Crambe Cordifolia and Crambe Koktebelica Leaves

Slobodianiuk, Liudmyla; Budniak, Liliia; Marchyshyn, S. М.; Skrynchuk, O. Ya.; Кудря, В. В.

doi:10.22159/ijap.2021v13i4.41265

Cited by 6 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Standards of amino acids, including L-asparagine, L-glutamic acid, L-alanine, L-leucine, L-serine, L-isoleucine, L-aspartic acid, L-valine, L-methionine, L-cysteine, L-phenylalanine, L-threonine, L-glutamine, L-proline, L-histidine, L-tryptophan, L-tyrosine, L-lysine, obtained from Sigma (Sigma-Aldrich, St. Louis, MO, USA), were of analytical grade (> 99 % purity) (Slobodianiuk et al 2019;Feshchenko et al 2021b;Slobodianiuk et al 2021f). All other reagents were of the highest purity.…”

Section: Standards and Chemicalsmentioning

confidence: 99%

Determination of amino acids content of the Tagetes lucida Cav. by GC/MS

Slobodianiuk¹,

Budniak²,

Marchyshyn³

et al. 2021

PHAR

Self Cite

View full text Add to dashboard Cite

Plant raw materials are widely used for the prevention and treatment providing of many diseases. The interest is the in-depth research of the flowers, leaves, and herb of Tagetes lucida. Therefore, the study aimed to determine the content of primary metabolites, namely amino acids in the raw materials of this plant. The amino acids composition and content in flowers, leaves, and herb were determined by the GC/MS method. The results of the study revealed that the raw material of Tagetes lucida contains more bound and less free amino acids. Free and bound L-proline, L-isoleucine were present in all the analyzed samples in the greatest amount (1.909 mg/g and 20.999 mg/g, 0.804 mg/g and 18.908 mg/g in the flowers; 2.721 mg/g and 18.973 mg/g, 3.459 mg/g and 28.518 mg/g in the leaves; 6.436 mg/g and 18.817 mg/g, 0.245 mg/g and 0.222 mg/g in the herb). Another free amino acid with a high content in flowers (1.321 mg/g) and herb (0.825 mg/g) of Tagetes lucida was L-aspartic acid. In addition, high content of L-phenylalanine in bound form was found in the leaves (11.843 mg/g) of the study plant. These amino acids to be considered distinguishing markers of the Tagetes lucida. This research contributes to already known information of Tagetes lucida use as herbal medicine, nutraceutical, and food reinforcement.

show abstract

Section: Standards and Chemicalsmentioning

confidence: 99%

Determination of amino acids content of the Tagetes lucida Cav. by GC/MS

Slobodianiuk¹,

Budniak²,

Marchyshyn³

et al. 2021

PHAR

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Standards and Chemicalsmentioning

confidence: 99%

Figure 1 from: Slobodianiuk L, Budniak L, Marchyshyn S, Kostyshyn L, Ezhned M (2021) Determination of amino acids content of the Tagetes lucida Cav. by GC/MS. Pharmacia 68(4): 859-867. https://doi.org/10.3897/pharmacia.68.e73325

Slobodianiuk¹,

Budniak²,

Marchyshyn³

et al. 2021

Self Cite

View full text Add to dashboard Cite

Determination of amino acids of some plants from Gentianaceae family

Budniak¹,

Slobodianiuk²,

Marchyshyn³

et al. 2021

PHAR

Self Cite

View full text Add to dashboard Cite

Medicines from plants are widely used in the complex treatment of different diseases every day. Therefore, theoretical and practical interest is the in-depth study of the herb of perspective plants like Centaurium erythraea Rafn. and Gentiana cruciata L. These plants have a long history of usage and interest of people. The aim of the study was to determine the content of amino acids in these plants. The amino acids composition and content in the herb of study species of the family Gentianaceae determined by the HPLC method. The results of the research revealed that the raw material of Centaurium erythraea Rafn. and Gentiana cruciata L. contains free and bound amino acids. Sixteen free and seventeen bound amino acids were identified in the herb of Centaurium erythraea Rafn. The herb of Gentiana cruciata L. contained thirteen free and fifteen bound amino acids. L-glutamic acid, L-arginine, L-aspartic acid, and L-cystine were predominant of Centaurium erythraea Rafn. herb. Amino acids L-lysine, L-serine, L-aspartic acid, and L-phenylalanine were present in the herb of Gentiana cruciata L. in the greatest amount. The metabolic processes in which these amino acids are involved connected to the medicinal properties of the study plants according to their use in official or nontraditional medicine.

show abstract

HPLC Analysis of Amino Acids Content in Crambe Cordifolia and Crambe Koktebelica Leaves

Cited by 6 publications

References 28 publications

Determination of amino acids content of the Tagetes lucida Cav. by GC/MS

Determination of amino acids content of the Tagetes lucida Cav. by GC/MS

Figure 1 from: Slobodianiuk L, Budniak L, Marchyshyn S, Kostyshyn L, Ezhned M (2021) Determination of amino acids content of the Tagetes lucida Cav. by GC/MS. Pharmacia 68(4): 859-867. https://doi.org/10.3897/pharmacia.68.e73325

Determination of amino acids of some plants from Gentianaceae family

Contact Info

Product

Resources

About