HPLC Analysis of Carotenoids from Tomatoes Using Cross-Linked C18 Column and MS Detection

Daood, Hussein G.; Bencze, Gyula; Palotás, Gábor; Pék, Zoltán; Sidikov, A. S.; Helyes, Lájos

doi:10.1093/chromsci/bmt139

Cited by 51 publications

(28 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, the organic solvent was evaporated under vacuum by rotary evaporator at not higher than 40°C. The residues were re-dissolved in HPLC acetone, as the best organic solvent that ensures high solubility of most of carotenoids before injection onto HPLC column [43].…”

Section: Extraction Of Carotenoidsmentioning

confidence: 99%

Using Infrared Spectroscopy for Tracking and Estimating Antioxidant in Tomato Fruit Fractions

Ibrahim¹,

Daood²,

Bori³

et al. 2018

EJERS

View full text Add to dashboard Cite

Infrared technology has brought a quantum leap in the specialization of non-destructive systems for internal quality inspection of agricultural and food products. Applying near-infrared spectroscopy technique (NIRs) for tracking and estimating some antioxidants such as (Lycopene, β-carotene, Phytoene and Phytofluenxe) in tomato fruit fractions (Exocarp, Mesocarp, Endocarp and Tomato pomace) with prediction model. High-performance liquid chromatography (HPLC) device showed the antioxidant concentrations values within tomato fractions. Where, the maximum and minimum values observed in the mesocarp and exocarp fractions. Also, tomato fractions color analysis confirmed these results. Meanwhile, mesocarp fraction within range dark red color with h°≈ 31.7°, due to increased lycopene concentration, whereas, exocarp fraction was 77.29° for h°, within yellow range. In addition to HPLC and color reference methods were consensus significantly with the different of spectral transformations by the regression of partial least square (PLS). NIR spectra and antioxidant in tomato fractions were taken to establish calibration models for tracking and estimating antioxidant in tomato fractions by using partial least squares (PLS) model. The obtained Coefficients of prediction model (R2p) were 0.95, 0.91, 0.93 and 0.94 for Lycopene, β-Carotene, Phytoene and Phytofluenxe respectively. The values of (RPD) ratio obtained from the standard deviation to the standard error of prediction and also (RER) obtained from the standard error range of prediction model were varied for different tomato fractions and antioxidant content, and found that the NIR model suitable not only for screening the different concentrations values of antioxidants for tomato fractions, but also suitable for most applications including quality assurance.

show abstract

Section: Extraction Of Carotenoidsmentioning

confidence: 99%

Using Infrared Spectroscopy for Tracking and Estimating Antioxidant in Tomato Fruit Fractions

Ibrahim¹,

Daood²,

Bori³

et al. 2018

EJERS

View full text Add to dashboard Cite

show abstract

“…Separation of carotenoids was performed on Nucleodur ISIS, 3 µm, 15 cm x 4.6 mm, column with gradient elution of (A) water and (B) acetone, according to Daood et al [30].…”

Section: Instrument and Hplc Conditionsmentioning

confidence: 99%

Effect of irrigation on yield parameters and antioxidant profiles of processing cherry tomato

et al. 2014

View full text Add to dashboard Cite

A two-year (2010 and 2011) open field experiment was conducted to study the effect of drip irrigation and seasonal variation on the yield parameters and main bioactive components, carotenoids (mainly all trans, cis lycopene, and β-carotene), polyphenols (chlorogenic acid, caffeic acid, gallic acid, quercetin, rutin, naringin, etc.), and tocopherols of processing Strombolino F1 cherry tomatoes. The irrigated plants (STI) gave a higher marketable yield (61% and 101% respectively), and rain fed plants showed a yield loss. Water supply had a strong positive (R2=0.98) effect on marketable yield in 2011, but weak (R2=0.69) in 2010. In both years, the antioxidant concentration (all carotenoids, total polyphenols, tocopherols) showed a decrease with irrigation. Water supply affected the composition of carotenoids to a considerable extent. The optimum water supply treatment gave a lower proportion of lycopene than the rain fed control (STC) treatment. We observed significant negative correlation between rutin concentration and irrigation. The α-tocopherol concentration was significantly higher in STC treatments. Irrigation negatively influenced antioxidant concentrations of cherry tomato fruits, but higher yield could account for the concentration loss of individual fruits by higher antioxidant production per unit area.

show abstract

“…Nonetheless, the complexation method can be concluded to be as good as solvent extraction, as both methods yielded carotenoid extract at a comparable level of purity. The broad peak at 8.9 min in the fraction from both extraction methods ( Figure 1 d) indicates the presence of β-carotene in both forms of trans and cis isomers [ 19 ]. Due to the overlapping peaks, the β-carotenes peaks were considered as a whole single peak.…”

Section: Resultsmentioning

confidence: 99%

Extraction of Carotenoids from Tomato Pomace via Water-Induced Hydrocolloidal Complexation

Nagarajan

Kay

K³

et al. 2020

Biomolecules

View full text Add to dashboard Cite

Agro-industrial waste is a largely untapped natural resource of bioactive compounds including carotenoids and pectin. However, conventional solvent extraction involves the excessive use of organic solvents, costly equipment, and tedious operation. These limitations of conventional extraction methods could be prospectively overcome by the carotenoid–pectin hydrocolloidal complexation. The complexation of lycopene and pectin was efficiently promoted in an aqueous environment, resulting in the colloidal complexes that can be subsequently recovered by sedimentation or centrifugation. In this study, the potential of carotenoid–pectin complexation on tomato pomace containing carotenoids and pectin was evaluated. Tomato pomace is a rich source of lycopene, β-carotene as well as pectin, making it suitable as the raw material for the carotenoid extraction. The extraction of carotenoid and pectin from tomato pomace was optimized using response surface methodology. The maximum recovery was 9.43 mg carotenoid fractions/100 g tomato pomace, while the purity of carotenoid-rich fractions was 92%. The antioxidant capacity of carotenoids extracted from the complexation method was found to be higher than that from the solvent extraction method. Moreover, extraction yield and antioxidant capacity of carotenoid obtained from the carotenoid–pectin complexation were comparable to that from solvent extraction. The carotenoid–pectin complexation is a promising green approach to valorize agro by-products for the extraction of valuable carotenoids.

show abstract

HPLC Analysis of Carotenoids from Tomatoes Using Cross-Linked C18 Column and MS Detection

Cited by 51 publications

References 30 publications

Using Infrared Spectroscopy for Tracking and Estimating Antioxidant in Tomato Fruit Fractions

Using Infrared Spectroscopy for Tracking and Estimating Antioxidant in Tomato Fruit Fractions

Effect of irrigation on yield parameters and antioxidant profiles of processing cherry tomato

Extraction of Carotenoids from Tomato Pomace via Water-Induced Hydrocolloidal Complexation

Contact Info

Product

Resources

About