HPLC Analysis ofMontanoaSpecies for Pharmacologically Active Constituents¹

Abstract

A reversed phase HPLC method was developed for the qualitative and quantitative analysis of selected biologically active diterpenoid constituents of methanolic and hot water extracts of some MONTANOA species.

Capillary electrophoretic method for the determination of diterpenoid isomers in Acanthopanax species

“…For effective utilization and quality control of the natural resources, analysis of the active compounds in Acanthopanax species could be very important. Although several reports have dealt with the determination of kaurenoic acid from Annona glabra by HPLC [19], from Xylopia species by HPLC [20], from Mikania glomerata by GC [21] or from Montanoa species by HPLC [22], no report about the simultaneous determination of diterpenoid isomers has been published. In our preliminary trials, acanthoic, kaurenoic and continentalic acids could not be separated simultaneously by HPLC because of their close molecular structure and physical properties (Fig.…”