HPLC analysis of non-enzymatic antioxidants in Azolla caroliniana (Pteridopsida) subjected to UV-B

Abstract

The ultrastructure of chloroplast and quantified antioxidant compounds such as α-tocopherol, ascorbic acid and beta carotene, semi quantitatively by comparing the peak areas of HPLC chromatograms, were affected considerably in response to exposure of Azolla caroliniana to UV-B radiation for 24 and 48 h. The results show that the most striking effect of the enhanced UV-B treatment on the ultrastructure of A. caroliniana was observed after 48 h as markedly widened chloroplast area occupied by large amount of plastoglobuli in stressed fronds. Prolonged UV-B exposure of A. caroliniana for 48 h resulted in a reduction in peaks area for for αtocopherol, ascorbic acid and beta-carotene. The results show that changes in the chromatogram of non-enzymatic antioxidants were correlated with the duration of exposure to UV-B radiation. These antioxidant metabolites play a vital role in protecting A. caroliniana to a certain limit against UV-B radiation.

The Evaluation and Comparison of Chemical Quality of β-Carotene Extracted in Different Seasons from Azolla filicloides

“…The results obtained in this study were in line with those obtained by Zarreh and Kianirad, that involved extraction of β-Carotene through fermenting the mold -Blakeslea tripsora [40], Razavai et al using fermentation method for poralomyces ruberrimus H110 via physicochemical processes [41], Baigan et al including the use of algae Donaliasalina by altering the culture medium [42] and Moghadasi et al applying soapy method with micro algae Donaliasalina [43,44] as well as the findings of Lejeune et al using solvent [44], Vennugopal et al who also applied solvent and Mustafa et al involving Tee method [45,46]. The results of present study are consistent with results of these researchers.…”