Hplc analysis of phenolic acids in mountain germander (Teucrium montanum L) extracts

Abstract

The methanol, petroleum ether, chloroform, ethyl acetate, 1-butanol and water extracts were obtained by extraction of mountain germander (Teucrium montanum L). The total phenolic content in extracts was measured by Folin-Ciocalteu method. The 1-butanol extract had the highest phenolic content (296.00 mg/g). High performance liquid chromatography (HPLC) was employed to define qualitative and quantitative content of phenolic acids in mountain germander extracts. The largest number of phenolic acids were determined in ethyl acetate and 1-butanol extracts, while these acids were not present in petroleum ether extract. The highest content of phenolic acids (28.619 mg/g) had ethyl acetate extract and gentisic acid (14.432 mg/g) was its major component. Despite of a large number of phenolic acids in 1-butanol extract their content was only 3.740 mg/g

Subcritical water extraction of antioxidants from mountain germander (Teucrium montanum L.)

“…On the other hand, vanillic acid (45.3 ± 5.1 mg/100 g DE) was recovered in less extent. To the best of our knowledge only few reports were found describing the phenolic composition of this plant [8,27,38]. Tumbas et al [27] investigated the influence of different solvents, namely methanol, petroleum ether, chloroform, ethyl acetate, 1-butanol and water on the TPC as well as the amount of individual phenolic compounds extracted.…”

“…To the best of our knowledge only few reports were found describing the phenolic composition of this plant [8,27,38]. Tumbas et al [27] investigated the influence of different solvents, namely methanol, petroleum ether, chloroform, ethyl acetate, 1-butanol and water on the TPC as well as the amount of individual phenolic compounds extracted. According to the authors, the 1-butanol extract had the highest TPC (296 mg/g), while the HPLC analysis revealed that the highest content of phenolic acids (28.619 mg/g) was found for ethyl acetate extract.…”

“…T. montanum is used as a diuretic, analgesic and antispasmodic agent, as well as in the treatment of digestive disorders and pulmonary diseases. Some of the bioactive compounds previously identified in T. montanum include phenolic acids, mainly gentisic, chlorogenic and siringic, flavonoids, sesquiterpenes, potassium, magnesium and sodium [10,[26][27][28]. According to Vukovic et al [28], high antimicrobial activity of T. montanum could be associated with sesquiterpenes, such as δ-cadinene and α-selinene.…”

Antimicrobial activity, total phenolic content and flavonoid concentrations of Teucrium species

“…Qualitative phytochemical analysis of Teucrium species revealed the presence of different secondary metabolites such as phenolic acids [10][11][12] and flavonoids with very strong biological activity [13,14]. However, little is known about the antimicrobial properties of Teucrium spp extracts.…”

Antimicrobial Activities of Essential Oil and Methanol Extract of Teucrium montanum

“…Teucrium montanum is a grass crop that has long been consumed both as a herbal medicine and as a nourishing food. It is widely used as diuretic, stomachic, analgesic and antispasmodic agent, and also possesses antibacterial, antifungal, anti-inflammatory and antioxidative activity [Jancic et al ( 4 ), Tumbas et al ( 5 )].…”