HPLC Analysis, Optimization of Extraction Conditions and Biological Evaluation of Corylopsis coreana Uyeki Flos

Abstract

Abstract:A method for the separation and quantification of three flavonoids and one isocoumarin by reverse-phase high performance liquid chromatography (HPLC) has been developed and validated. Four constituents present in a crude ethanolic extract of the flowers of Coryloposis coreana Uyeki, were analyzed. Bergenin, quercetin, quercitrin and isosalipurposide were used as calibration standards. In the present study, an excellent linearity was obtained with an r 2 higher than 0.999. The chromatographic peaks showed good resolution. In combination with other validation data, including precision, specificity, and accuracy, this method demonstrated good reliability and sensitivity, and can be conveniently used for the quantification of bergenin, quercetin, quercitrin and isosalipurposide in the crude ethanolic extract of C. coreana Uyeki flos. Furthermore, the plant extracts were analyzed with HPLC to determine the four constituents and compositional differences in the extracts obtained under different extraction conditions. Several extracts of them which was dependent on the ethanol percentage of solvent were also analyzed for their antimicrobial and antioxidant activities. One hundred % ethanolic extract from C. coreana Uyeki flos showed the best antimicrobial activity against the methicillin-resistant Staphylococcus aureus (MRSA) strain. Eighty % ethanolic extract showed the best antioxidant activity and phenolic content. Taken of all, these results suggest that the flower of C. coreana Uyeki flos may be a useful source for the cure and/or prevention of septic arthritis, and the validated method was useful for the quality control of C. coreana Uyeki.

Effects of extracts from Corylopsis coreana Uyeki (Hamamelidaceae) flos on xanthine oxidase activity and hyperuricemia

“…In our previous study, 80% ethanolic extract showed optimal 2,2diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and phenolic content. [10] Thus, based on the results of our previous study and the findings of the current study, the 80% ethanolic extract was selected for further in-vitro and in-vivo studies.…”

“…Our previous study revealed that BER, ISO, QCT and QCIT are the main bioactive phytochemicals identified from C. coreana Uyeki flos. [10] Thus, these four constituents were analysed in the 80% ethanolic extract that was prepared in this study. BER was one of the major compounds in the 80% ethanolic extract of C. coreana Uyeki flos.…”

“…Constituent profiling of C. coreana Uyeki flos samples by high‐performance liquid chromatography (HPLC) analysis was conducted as previously described . All analyses were performed using an Alliance 2695 HPLC system (Waters; Millford, MA, USA) equipped with a photodiode array detector.…”

“…Constituent profiling of C. coreana Uyeki flos samples by high-performance liquid chromatography (HPLC) analysis was conducted as previously described. [10] All analyses were performed using an Alliance 2695 HPLC system (Waters; Millford, MA, USA) equipped with a photodiode array detector. The analytical column used was an Agilent Zorbax extended C18 (5 lm, 150 mm 9 5 mm) with a mobile phase consisting of solvent A (acetonitrile) and B (water containing 0.2% phosphoric acid) and employing gradient elution (from 10/90 to 100/0, v/v) at a flow rate of 0.8 ml/min ( Table 1).…”

“…In a previous study, 15 compounds from the leaves of C. coreana Uyeki were identified, and their antioxidative and antiproliferative properties were evaluated . Recently, we identified quercetin (QCT), quercitrin (QCIT), bergenin (BER) and isosalipurposide (ISO) in the flower extract of C. coreana Uyeki and reported its antimicrobial and antioxidant activity . However, to the best of our knowledge, there have been no studies on the effects of C. coreana Uyeki and its phytochemicals on XO activity and hyperuricemia.…”