HPLC and GC-MS identification of the major organic constituents in New Zeland propolis

Markham, Kenneth R.; Mitchell, Kevin; Wilkins, Alistair L.; Daldy, Julia A.; Lu, Ying

doi:10.1016/0031-9422(96)83286-9

Cited by 198 publications

(130 citation statements)

References 18 publications

Supporting

Mentioning

108

Contrasting

Unclassified

Order By: Relevance

“…Most widely studied components are flavonoids and phenolic compounds and reported as a type of antioxidants that has strong inhibitory effect against lipid oxidation by radicalscavenging. In addition, the contents of flavonoid and other phenolic substances may also prevent the development of cancer and heart disease [16,17].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical analysis and antioxidant activities of Trigona Apicalis propolis extract

Rosli¹,

Roslan²,

Omar³

et al. 2016

AIP Conference Proceedings

View full text Add to dashboard Cite

Abstract. Propolis is a resinous substance found in beehives. It provides beneficial effects on human health and has been used to treat many diseases since ancient times. The objectives of this study were to analyze the phytochemical profile of propolis derived from local T. apicalis species and its antioxidant activities. The ethanolic extract of propolis was subjected to HPLC analysis to analyze its phytochemical profile. The propolis extract was later tested for antioxidant capacities by using DPPH radical scavenging assay. TPC and TFC were performed to determine the correlation with its antioxidant activities. TEAC for each serial dilution sample was 2621.15 (4.76 mg/mL), 2050.85 (2.38 mg/mL), 1883.27 (1.19 mg/mL), 1562.67 (0.59 mg/mL), 1327.82 (0.29 mg/mL), 1164.49 (0.15 mg/mL), 983.27 (0.07 mg/mL), and 944.79 (0.04 mg/mL). The results demonstrated that the antioxidant activities of propolis extract were dose dependent. The IC50 of propolis for DPPH assay was 4.27 mg/ml. Correlation values of TPC and TFC against DPPH indicate that the antioxidant activities of propolis extract used in this study could be mainly influenced by the phenolic and flavonoid contents. These findings highlighted the importance of quality analysis in order to ensure the consistency of biological effects or therapy of a natural product, such as propolis.

show abstract

Section: Introductionmentioning

confidence: 99%

Phytochemical analysis and antioxidant activities of Trigona Apicalis propolis extract

Rosli¹,

Roslan²,

Omar³

et al. 2016

AIP Conference Proceedings

View full text Add to dashboard Cite

show abstract

“…Some studies have reported on the composition of A. mellifera propolis from other regions of the world such as New Zealand [7] and Korea [8], including flavonoids as important constituents of propolis from these regions. For the majority of Asian or African countries, however, the composition and plant origins of propolis samples are only recently being studied [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…For the majority of Asian or African countries, however, the composition and plant origins of propolis samples are only recently being studied [9][10][11]. Both gas chromatography coupled to mass spectrometry (GC/MS) [7] and high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC/ESI-MS) have been used to compare A. mellifera propolis from different regions [9,10] as well as its plant origins [12].…”

Section: Introductionmentioning

confidence: 99%

Fingerprinting of propolis by easy ambient sonic-spray ionization mass spectrometry

Sawaya

Abdelnur²,

Eberlin³

et al. 2010

Talanta

View full text Add to dashboard Cite

a b s t r a c tChemical profiles of a representative set of 49 propolis ethanolic extracts collected worldwide (North and South America, Europe, Asia and Oceania) were obtained via easy ambient sonic-spray ionization mass spectrometry (EASI-MS). This simple and easily implemented fingerprinting technique analyses directly (without any pre-separation or sample manipulation) a tiny droplet of the ethanolic extract placed on a inert surface under ambient conditions. Data acquisition took about a minute per sample with no substantial sample carry-over. Extraction of propolis with ethanol by using an ultrasonic bath method gave EASI-MS data similar to the traditional maceration method, reducing total analysis time for the crude propolis resin from a week to just ca 1 h. Principal component analysis of the EASI-MS data is shown to group samples according to the plant sources of their resins, which characterizes their geographical origin.

show abstract

“…Na Europa, América do Norte e oeste da Ásia, a fonte dominante de própolis é o exsudato do botão de álamo (Populus sp.) (MARKHAM et al, 1996;WOLLENWEBER & BUCHMANN, 1997). Entretanto, na América do Sul a espécie vegetal do gênero Populus não é nativa, existindo uma grande diversidade vegetal para a retirada de resina, o que dificulta a correlação da própolis com a fonte produtora.…”

Section: Introductionunclassified

Própolis produzida no sul do Brasil, Argentina e Uruguai: evidências fitoquímicas de sua origem vegetal

et al. 2002

View full text Add to dashboard Cite

show abstract

HPLC and GC-MS identification of the major organic constituents in New Zeland propolis

Cited by 198 publications

References 18 publications

Phytochemical analysis and antioxidant activities of Trigona Apicalis propolis extract

Phytochemical analysis and antioxidant activities of Trigona Apicalis propolis extract

Fingerprinting of propolis by easy ambient sonic-spray ionization mass spectrometry

Própolis produzida no sul do Brasil, Argentina e Uruguai: evidências fitoquímicas de sua origem vegetal

Contact Info

Product

Resources

About