HPLC–DAD–FLD Determination of Veterinary Pharmaceuticals in Pharmaceutical Industry Wastewater with Precolumn Derivatization Using Fluorescamine

Ašperger, Danijela; Tišler, Vesna; Zrnčić, Mirta; Pavlović, Dragana Mutavdžić; Babić, Sandra; Horvat, Alka J.M.; Kaštelan‐Macan, Marija

doi:10.1007/s10337-014-2685-x

Cited by 20 publications

(11 citation statements)

References 29 publications

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“…The performance of the developed method was compared with that from previous studies (Table ). As for HPLC–FLD detection for SA analysis, the sensitivity levels recorded in this study were in the same order of those determined using other extraction methods such as in situ derivatization by pyridine/acetic anhydride and HF‐LPME , in situ derivatization by fluorescamine and IL‐based MADLLME , and SPE with precolumn derivatization by fluorescamine . Regarding the applied HF‐LPME method, the LOQs of SAs in our study were slightly lower than those without derivatization in previous studies .…”

Section: Resultssupporting

confidence: 83%

“…The most widely used detection method equipped with LC for SAs quantification is MS/MS . Additionally, diode array detection (DAD) , UV detection , and fluorescence detection (FLD) are frequently used. DAD and UV detectors are common but their sensitivities are limited; by contrast, MS/MS is efficient and sensitive but also expensive .…”

Section: Introductionmentioning

confidence: 99%

“…SAs exhibit low fluorescent properties, and thus require derivatization for adding a fluorophore to facilitate FLD detection. SAs have been derivatized to improve method sensitivity and selectivity in situ [11,[28][29][30], in both precolumn [22][23][24][31][32][33][34] and post-column mode [35]. The most common labeling reagent is fluorescamine, which is readily available and inexpensive [33].…”

mentioning

confidence: 99%

“…Thus far, HF-LPME has been applied to SAs extraction from water sample through CE with electrochemical detection (ECD) [36], HPLC-DAD-FLD [37], UHPLC-MS/MS [38], HPLC-UV [26], and also from human urine through HPLC-DAD-FLD [39]. Further, FLD has been widely applied in analyzing SAs through pre-column or postcolumn derivatization in different matrixes such as honey [22,29,30,32], meat [33][34][35]40,41], milk [23,30,40,42,43], animal plasma [30,44], animal feed [31], eggs [34,41,45], and water samples [24,30].…”

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confidence: 99%

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In situ derivatization and hollow‐fiber liquid‐phase microextraction to determine sulfonamides in water using UHPLC with fluorescence detection

Yang

Shi

Li³

et al. 2018

J of Separation Science

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A sensitive method for determining sulfonamides in water was developed and validated through in situ derivatization and hollow-fiber liquid-phase microextraction with ultra-high performance liquid chromatography and fluorescence detection. The target sulfonamides were sulfadiazine, sulfacetamide, sulfamerazine, sulfamethazine, sulfamethoxypyridazine, sulfachloropyridazine, sulfamethoxazole, and sulfisoxazole. Following in situ derivatization with fluorescamine, three-phase hollow-fiber liquid-phase microextraction with an S 6/2 polypropylene hollow-fiber membrane was applied automatically using a multipurpose autosampler. Experimental parameters including derivatization time, choice of organic phase, pH of donor and acceptor phase, stirring rate, extraction temperature and time were optimized. Under optimized conditions, the target sulfonamides achieved excellent linearity with correlation coefficients of 0.9924-0.9994 within the concentration range of 0.05-5 μg/L. The limits of detection of the eight sulfonamides were 3.1-11.2 ng/L, and the limits of quantification were 10.3-37.3 ng/L. Enrichment factors of 0.1 and 5 μg/L sulfonamides spiked in lake water were 14-60, and recoveries were 56-113% with relative standard derivations of 3-19%. Applied with the developed method, sulfamerazine and sulfamethoxazole were measurable in both influent and effluent water of the three sewage treatment plants in Guangzhou, China. The developed method was sensitive and provided an alternative method for simultaneously enriching and quantifying multiple sulfonamides in environmental water.

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Section: Resultssupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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In situ derivatization and hollow‐fiber liquid‐phase microextraction to determine sulfonamides in water using UHPLC with fluorescence detection

Yang

Shi

Li³

et al. 2018

J of Separation Science

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“…More generally, pharmaceuticals move into the sewage system and to waste water treatment plants [10]. The nutrition-rich sewage sludge and compost can be used as fertilizers for plants.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Fluoroquinolones and Sulfonamides Originating from Sewage Sludge Compost

Kipper

Lillenberg

Herodes

et al. 2017

The Scientific World Journal

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A simultaneous method for quantitative determination of traces of fluoroquinolones (FQs) and sulfonamides (SAs) in edible plants fertilized with sewage sludge was developed. The compounds were extracted from the plants by rapid and simple liquid extraction followed by extracts clean-up using solid phase extraction. The eluent additive 1,1,1,3,3,3-hexafluoro-2-propanol was used for liquid chromatographic detection to achieve separation of structurally similar antimicrobials like ciprofloxacin and norfloxacin. Identification and quantification of the compounds were performed using high-performance liquid chromatography with electrospray ionization mass spectrometry in selected reaction monitoring mode. Method was validated and extraction recoveries of FQs and SAs ranged from 66% to 93%. The limit of quantifications was from 5 ng/g in the case of ofloxacin to 40 ng/g for norfloxacin. The method precision ranged from 1.43% to 2.61%. The developed novel method was used to evaluate the plats antimicrobial uptake (potato (Solanum tuberosum L.), carrot (Daucus carota L.), lettuce (Lactuca sativa L.), and wheat (Triticum vulgare L.)) from soil and migration of the analytes inside the plants.

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Use of hydroxypropyl β‐cyclodextrin hybrid monolithic material as adsorbent for dispersive solid‐phase extraction of fluoroquinolones from environmental water samples

Zhou

2022

J of Separation Science

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In this study, the hydroxypropyl β-cyclodextrin hybrid monolithic material was fabricated and firstly applied as an adsorbent for dispersive solidphase extraction coupled with high-performance liquid chromatography to detect trace-level seven fluoroquinolones in water samples. The prepared hydroxypropyl β-cyclodextrin hybrid monolithic material was characterized by Fourier-transform infrared spectroscopy, scanning electron microscopy, and adsorption experiments, which showed excellent specific adsorption to the target fluoroquinolones. Under the optimized conditions, the extraction methodology showed satisfactory precision with relative standard deviations between 2.6% and 5.6%, good linearity (R 2 ≥ 0.9990), and satisfactory recoveries (82.5-91.8%).The limits of detection and limits of quantification of the method were in the range of 0.4-1.2 and 1.4-4.0 ng/mL, respectively, which confirmed the possibility of quantifying trace levels. Furthermore, the material could be reused at least five times. These results demonstrated that the hydroxypropyl β-cyclodextrin hybrid monolithic material was a promising adsorbent for fluoroquinolones, and the established method combined dispersive solid-phase extraction with highperformance liquid chromatography was suitable for the determination of fluoroquinolones in aqueous samples.

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HPLC–DAD–FLD Determination of Veterinary Pharmaceuticals in Pharmaceutical Industry Wastewater with Precolumn Derivatization Using Fluorescamine

Cited by 20 publications

References 29 publications

In situ derivatization and hollow‐fiber liquid‐phase microextraction to determine sulfonamides in water using UHPLC with fluorescence detection

In situ derivatization and hollow‐fiber liquid‐phase microextraction to determine sulfonamides in water using UHPLC with fluorescence detection

Simultaneous Determination of Fluoroquinolones and Sulfonamides Originating from Sewage Sludge Compost

Use of hydroxypropyl β‐cyclodextrin hybrid monolithic material as adsorbent for dispersive solid‐phase extraction of fluoroquinolones from environmental water samples

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