In situ derivatization and hollow‐fiber liquid‐phase microextraction to determine sulfonamides in water using UHPLC with fluorescence detection

Abstract

A sensitive method for determining sulfonamides in water was developed and validated through in situ derivatization and hollow-fiber liquid-phase microextraction with ultra-high performance liquid chromatography and fluorescence detection. The target sulfonamides were sulfadiazine, sulfacetamide, sulfamerazine, sulfamethazine, sulfamethoxypyridazine, sulfachloropyridazine, sulfamethoxazole, and sulfisoxazole. Following in situ derivatization with fluorescamine, three-phase hollow-fiber liquid-phase microextraction with an S 6/2 polypropylene hollow-fiber membrane was applied automatically using a multipurpose autosampler. Experimental parameters including derivatization time, choice of organic phase, pH of donor and acceptor phase, stirring rate, extraction temperature and time were optimized. Under optimized conditions, the target sulfonamides achieved excellent linearity with correlation coefficients of 0.9924-0.9994 within the concentration range of 0.05-5 μg/L. The limits of detection of the eight sulfonamides were 3.1-11.2 ng/L, and the limits of quantification were 10.3-37.3 ng/L. Enrichment factors of 0.1 and 5 μg/L sulfonamides spiked in lake water were 14-60, and recoveries were 56-113% with relative standard derivations of 3-19%. Applied with the developed method, sulfamerazine and sulfamethoxazole were measurable in both influent and effluent water of the three sewage treatment plants in Guangzhou, China. The developed method was sensitive and provided an alternative method for simultaneously enriching and quantifying multiple sulfonamides in environmental water.

Recent Advances and Perspectives on the Sources and Detection of Antibiotics in Aquatic Environments

“…At the same time, the method was proved by simultaneous determination of nine FQs and quinolone pipemidic acid in urban wastewater, which was successfully applied in FQ quantification of urban sewage treatment plant effluent. In 2018, Yang [ 60 ] and others established an ultraperformance liquid chromatography-fluorescence method for the determination of sulfonamides in water. The detection of target sulfonamides was linear in the concentration range of 0.05–5 μ g/L, and the correlation coefficient was 0.9924–0.9994.…”

Synthesis of the Magnetically Nanoporous Organic Polymer Fe3O4@SiO2‐NH2‐COP and Its Application in the Determination of Sulfonamide Residues in Surface Water Surrounding a Cattle Farm

“…The ultraviolet (UV) detector has been the most commonly applied one for HPLC because of its price advantage [ 18 ]. Other sensitive detectors, such as fluorescence [ 19 ] and mass spectrometry (MS), [ 20 , 21 ] have been applied to improve the detection sensitivity. These detectors are, however, relatively expensive for maintenance.…”

Development of an amplified luminescent proximity homogeneous assay for the detection of sulfonamides in animal‐derived products

“…Several instrumental analysis techniques have been proposed for the determination of SAs residue (Premarathne et al., 2017 ; Wang et al., 2019 ; Xie et al., 2020 ; Yang et al., 2018 ; Zhao et al., 2018 ), including liquid chromatography (LC), liquid chromatography–tandem mass spectrometry (LC‐MS/MS), and ultra‐high‐performance supercritical fluid chromatography (SFC). All these detection techniques have earned robust recognition in SAs and have many advantages, such as large dynamic linear range, low detection limits, and high productivity.…”