HPLC-DAD-MS/MS profiling of phenolics from different varieties of peach leaves and evaluation of their antioxidant activity: A comparative study

Mokrani, Abderrahmane; Cluzet, Stéphanie; Madani, Khodir; Pakina, Elena; Gadzhikurbanov, Anvar; Mesnil, Marc; Monvoisin, Arnaud; Richard, Tristan

doi:10.1016/j.ijms.2019.116192

Cited by 23 publications

(18 citation statements)

References 27 publications

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“…In this regard, excluding tyrosols, each polyphenol class analyzed was significantly correlated (p < 0.01) to the DPPH, FRAP, CUPRAC, and ABTS assays. In accordance with our results, polyphenols were reported as the main contributors to antioxidant properties in earlier studies [41,42]. On the other hand, only flavonoids (i.e., anthocyanins, flavanols, flavonols, and flavones) were found to be highly correlated to the enzymatic inhibition tested, with the most significant values (p < 0.01) recorded for flavanols (Supplementary Materials).…”

Section: Correlationssupporting

confidence: 91%

Chemical Profiling and Biological Properties of Extracts from Different Parts of Colchicum Szovitsii Subsp. Szovitsii

et al. 2019

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Like other members of the Colchicum genus, C. szovitsii subsp. szovitsii is also of medicinal importance in Turkish traditional medicine. However, its biological properties have not been fully investigated. Herein, we focused on the evaluation of the in vitro antioxidant and enzyme inhibitory effects of flower, root and leaf extracts, obtained using different extraction methods. In addition, a comprehensive (poly)-phenolic and alkaloid profiling of the different extracts was undertaken. In this regard, ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) allowed us to putatively annotate 195 polyphenols and 87 alkaloids. The most abundant polyphenols were flavonoids (83 compounds), whilst colchicine and 2-demethylcolchicine were some of the most widespread alkaloids in each extract analyzed. However, our findings showed that C. szovitsii leaf extracts were a superior source of both total polyphenols and total alkaloids (being, on average 24.00 and 2.50 mg/g, respectively). Overall, methanolic leaf extracts showed the highest (p < 0.05) ferric reducing antioxidant power (FRAP) reducing power (on average 109.52 mgTE/g) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging (on average 90.98 mgTE/g). Interestingly, each C. szovitsii methanolic extract was more active than the water extracts when considering enzymatic inhibition such as against tyrosinase, glucosidase, and acetylcholinesterase (AChE). Strong correlations (p < 0.01) were also observed between polyphenols/alkaloids and the biological activities determined. Multivariate statistics based on supervised orthogonal projections to latent structures discriminant analysis (OPLS-DA) allowed for the detection of those compounds most affected by the different extraction methods. Therefore, this is the first detailed evidence showing that C. szovitsii subsp. szovitsii might provide beneficial effects against oxidative stress and the associated chronic diseases. Nevertheless, the detailed mechanisms of action need to be further investigated.

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Section: Correlationssupporting

confidence: 91%

Chemical Profiling and Biological Properties of Extracts from Different Parts of Colchicum Szovitsii Subsp. Szovitsii

et al. 2019

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“…These findings suggest that the activity of antioxidants is affected by environmental conditions, plant parts, stage of maturity, method of harvesting, and solvents used for extraction. These results are consistent with the literature as PP leaf extract was found to have a better antioxidant capacity than seed, peel, pulp, and fruit extracts in both assays [ 14 , 20 , 50 ]. Also, the reported high flavonoidal content of PP leaves, in particular, flavonols was thought to be responsible for the antioxidant activity [ 20 ].…”

Section: Resultssupporting

confidence: 92%

“…PPEE showed high concentrations of phenolic and flavonoid compounds. Both results are following values previously reported for PP leaf extracts confirming that flavonoids are the main chemical constituents in PP leaves [ 20 ].…”

Section: Resultssupporting

confidence: 91%

“…Traditionally, its leaves have been used as diuretics, laxatives, vermifuges, insecticides, sedatives, for whooping cough, for the treatment of leukoderma, and as febrifuges [ 17 ]. Furthermore, pharmacological studies on leaves characterized their in-vivo anti-diabetic, spasmogenic effect, anti-inflammatory, anticoagulant, hepatoprotective, antimalarial, anti-asthmatic, and in-vitro cytotoxic, antimicrobial, and nitric oxide inhibitory with significant anti-oxidant activities [ 18 , 19 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…To date, a single report is available describing the isolation of five flavonol glycosides from an ethanol leaves extract [ 25 ]. In addition, phenolic compounds have been characterized, including phenolic acids and flavonoids using HPLC-MS analysis with flavonols as dominant substances among all the determined compounds [ 20 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

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A Unique Acylated Flavonol Glycoside from Prunus persica (L.) var. Florida Prince: A New Solid Lipid Nanoparticle Cosmeceutical Formulation for Skincare

et al. 2021

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Polyphenols are known dietary antioxidants. They have recently attracted considerable interest in uses to prevent skin aging and hyperpigmentation resulting from solar UV-irradiation. Prunus persica (L.) leaves are considered by-products and were reported to have a remarkable antioxidant activity due to their high content of polyphenols. This study aimed at the development of a cosmeceutical anti-aging and skin whitening cream preparation using ethanol leaves extract of Prunus persica (L.) (PPEE) loaded in solid lipid nanoparticles (SLNs) to enhance the skin delivery. Chemical investigation of PPEE showed significantly high total phenolic and flavonoids content with notable antioxidant activities (DPPH, ABTS, and β-carotene assays). A unique acylated kaempferol glycoside with a rare structure, kaempferol 3-O-β-4C1-(6″-O-3,4-dihydroxyphenylacetyl glucopyranoside) (KDPAG) was isolated for the first time and its structure fully elucidated. It represents the first example of acylation with 3,4-dihydroxyphenyl acetic acid in flavonoid chemistry. The in-vitro cytotoxicity studies against a human keratinocytes cell line revealed the non-toxicity of PPEE and PPEE-SLNs. Moreover, PPEE, PPEE-SLNs, and KDPAG showed good anti-elastase activity, comparable to that of N-(Methoxysuccinyl)-Ala-Ala-Pro-Val-chloromethyl ketone. Besides, PPEE-SLNs and KDPAG showed significantly (p < 0.001) higher anti-collagenase and anti-tyrosinase activities in comparison to EDTA and kojic acid, respectively. Different PPEE-SLNs cream formulae (2% and 5%) were evaluated for possible anti-wrinkle activity against UV-induced photoaging in a mouse model using a wrinkle scoring method and were shown to offer a highly significant protective effect against UV, as evidenced by tissue biomarkers (SOD) and histopathological studies. Thus, the current study demonstrates that Prunus persica leaf by-products provide an interesting, valuable resource for natural cosmetic ingredients. This provides related data for further studying the potential safe use of PPEE-SLNs in topical anti-aging cosmetic formulations with enhanced skin permeation properties.

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HPLC–ESI/MS profiling, phytoconstituent isolation and evaluation of renal function, oxidative stress and inflammation in gentamicin‐induced nephrotoxicity in rats of Ficus spragueana Mildbr. & Burret

Taher

Raslan

Masoud

et al. 2021

Biomedical Chromatography

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Ficus spragueana Mildbr. & Burret (family Moraceae) was reported to have various biological activities. However, its activity in treatment of renal injury has not been investigated yet. The current study aimed to evaluate the effects of F. spragueana leaf extract on nephrotoxicity caused by gentamicin. Gentamicin is an important broad‐spectrum antibiotic; nevertheless, it exhibits serious nephrotoxic adverse effects. HPLC–ESI/MS spectrometric analysis of the extract revealed the presence of 37 phenolic compounds. Moreover, five compounds were isolated from the leaf extract, and identified on the basis of spectroscopic analysis. The isolated compounds were syringic acid (1), p‐coumaric acid (2), 3′,5′ O‐dicaffeoylquinic acid (3), luteolin‐8‐C‐β‐D glucopyranoside (orientin) (4) and 8‐methoxy kaempferol‐3‐O‐[α‐L‐rhamnopyranosyl (1→2) β‐D‐glucopyranoside] (5). The gentamicin‐induced nephrotoxicity model was used to evaluate the protective effect of F. spragueana on renal toxicity biomarkers throughout the development of acute kidney injury. Administration of extract led to improvement in kidney function through inhibition of kidney injury molecule‐1, creatinine, blood urea nitrogen and total bilirubin, as well as decreasing the inflammatory markers interlukin1‐beta and myeloperoxidase. Furthermore, it reduced the oxidative stress by increasing reduced glutathione and total antioxidant capacity levels while decreasing malondialdehyde and nitric oxide content, and improved renal histopathological injuries.

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HPLC-DAD-MS/MS profiling of phenolics from different varieties of peach leaves and evaluation of their antioxidant activity: A comparative study

Cited by 23 publications

References 27 publications

Chemical Profiling and Biological Properties of Extracts from Different Parts of Colchicum Szovitsii Subsp. Szovitsii

Chemical Profiling and Biological Properties of Extracts from Different Parts of Colchicum Szovitsii Subsp. Szovitsii

A Unique Acylated Flavonol Glycoside from Prunus persica (L.) var. Florida Prince: A New Solid Lipid Nanoparticle Cosmeceutical Formulation for Skincare

HPLC–ESI/MS profiling, phytoconstituent isolation and evaluation of renal function, oxidative stress and inflammation in gentamicin‐induced nephrotoxicity in rats of Ficus spragueana Mildbr. & Burret

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