HPLC determination of biogenic amines in discrete brain areas in food deprived rats

Loullis, Costas C.; Felten, David L.; Shea, P.A.

doi:10.1016/0091-3057(79)90302-2

Cited by 65 publications

(16 citation statements)

References 11 publications

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“…Alternatively, Maruyama et al (1980) used N-methyldopamine as internal standard for brain assays with reverse-phase separation where DHBA and epinephrine overlap. N-methyl-5-HT (Loullis et al, 1979) and 3-methoxy-4-hydroxyphenethyl alcohol (Mefford, 1981) have proved to be successful internal standards for indoleamines with reverse-phase ion-pair separation. The internal standard should be added at the start of the extraction process ( Fig.…”

Section: Internal Standards and Recovery Determinationsmentioning

confidence: 99%

Electrochemical Detection Methods for Monoamine Measurements in Vitro and in Vivo

Adams

Marsden

1982

Handbook of Psychopharmacology

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Section: Internal Standards and Recovery Determinationsmentioning

confidence: 99%

Electrochemical Detection Methods for Monoamine Measurements in Vitro and in Vivo

Adams

Marsden

1982

Handbook of Psychopharmacology

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“…After washing with 2 ml of bidistilled water the indoles were finally obtained by application of 5.0 ml of sodium acetatelcitrate buffer (0.1 mmol/l, pH 4.3) containing 85% (v/v) methanol. Ten microliters of the dried extract dis-solved in perchloric acid (100 ~1 , 0.1 mol/l) was used for high performance liquid chromatography modified from Loullis et al (12) and Mefford and Barchas (13). The stationary phase was a Bondapack C18, 1 0 -~, column (4.6 x 250 mm, Waters Ass., Milford, MA); the mobile phase was the above mentioned citratelacetate buffer but with 5% methanol (v/v).…”

Section: Methodsmentioning

confidence: 99%

Increase of Tryptophan and 5-Hydroxyindole Acetic Acid in the Brain of Ornithine Carbamoyltransferase Deficient Sparse-Fur Mice

Bachmann

Colombo

1984

Pediatr Res

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Summaryeated in that complex model (1 1). For this reason we used a Sparse-fur mice, 28 d of age with the x-chromosomal inherited defect of ornithine carbamoyltransferase, were used to investigate if tryptophan and the serotonin pathway in the brain are affected in this animal model which closely resembles the human inborn error of metabolism. Increased concentrations of tryptophan and 5-hydroxyindole acetic acid were found in forebrain and brainstem. Application of probenecid, which blocks the efflux of 5-hydroxyindole acetic acid from the brain, led to an augmented accumulation of this serotonin metabolite in the affected males. We conclude that the increased concentration of tryptophan in brain and the subsequent increased flux through the serotonin pathway are a consequence of hyperammone@a in this inherited defect of urea synthesis. Surprisingly, increased carbamoylphosphate synthetase was found in the liver of the male sparse-fur mice. Abbreviations CPS, carbamoylphosphate synthetase HIAA, 5-hydroxyindole acetic acid HT, 5-hydroxytryptamine NAGS, N-acetylglutamate synthetase OCT, ornithine carbamoyltransferase spf, sparse-fur TRP, tryptophan Sparse-fur (spf) mice have a x-chromosomal inherited defect of OCT (E C 2.3.3.1 1). In affected males this leads to hyperammonemia (10, 17) as in the human variant of OCT deficiency, an inborn error of metabolism which presents with neurologic symptoms (1, 23). These are presumably mediated by changes of neurotransmitters in brain. We chose to investigate metabolites of the serotonin pathway in spf mice, because TRP and HIAA were increased in two animal models where hyperammonemia is present. Studies with rats subjected to portacaval shunting have repeatedly led to the conclusion that chronic liver insufficiency (with hyperammonemia) increases the brain concentration of TRP, the precursor of serotonin (HT), and of HIAA, the main metabolite of HT (1 1). Because of the multiple metabolic changes encountered in portacaval anastomosis, the particular role of hyperammonemia has not been clearly delinsimpler set-up and injected rats with urease. We found that the transport of TRP into the brain was enhanced and that the concentrations of TRP and HIAA in forebrain and brainstem were higher than in pair-fed controls (3).The following investigations were performed to find out if an inherited defect of an urea cycle enzyme leads to comparable changes in the serotonin pathway. We examined if, in the spf mouse model, the affected males (spf/Y) had increased concentrations of TRP, HT, and HIAA in brain as compared with their unaffected littermates (+/Y). In order to detect if the flux through the serotonin pathway is altered in spf mice, the indole metabolites were determined also after injection of probenecid, an inhibitor of HIAA transport out of the brain. MATERIALS AND METHODSSpf-females (spf/+) from the Oak Ridge National Laboratory (Oak Ridge, TN) were mated with C57BL*BALBc males (Jackson Lab, Bar Harbour, ME). The offspings were used for the experiments. These were camed ou...

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“…Thus, in our study 7 out of 13 partially trans planted rats survived throughout the study (54%). In contrast, survival rates for whole liver transplantations are reported in the range of 70-80% [ 17], The neurological test has been useful for the study of ammonium salt deposits in the brain [12][13][14], It therefore reflects the func tion of the liver. The test also describes objec tively the general condition of the animal.…”

Section: Discussionmentioning

confidence: 99%

“…The function of the transplanted liver graft w'as evaluated by a noninvasive neurological test [12][13][14] on day 1.2, 4, 15 and 23 after the operation. The 15-point neurological test examined the following re flexes.…”

Section: Neurological Test Performancementioning

confidence: 99%

Liver Regeneration following 68&percnt; Partial Orthotopic Liver Transplantation in the Rat

Foss

Zoucas²,

Steinbauer³

et al. 1993

Eur Surg Res

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Liver regeneration following transplantation in ‘small for size’ conditions is not fully understood. We therefore evaluated the regenerative response of transplanted partial liver grafts in outbred rats without the use of immunosuppression and compared it to liver regeneration following resection. The transplanted livers showed enhanced regeneration compared to controls. We suggest that this is caused by activation of the immune system.

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HPLC determination of biogenic amines in discrete brain areas in food deprived rats

Cited by 65 publications

References 11 publications

Electrochemical Detection Methods for Monoamine Measurements in Vitro and in Vivo

Electrochemical Detection Methods for Monoamine Measurements in Vitro and in Vivo

Increase of Tryptophan and 5-Hydroxyindole Acetic Acid in the Brain of Ornithine Carbamoyltransferase Deficient Sparse-Fur Mice

Liver Regeneration following 68&percnt; Partial Orthotopic Liver Transplantation in the Rat

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