HPLC determination of chlorobenzenes, benzenesulphonyl chlorides and benzenesulphonic acids in industrial waste water

Abstract

SummaryAn HPLC method has been developed for the determination of chlorobenzenes, benzenesulphonyl chlorides, benzenesulphonic acids and related compounds in industrial waste water. The separation and determination of diphenylsulphone, 1,4-dichlorobenzene, 1,2,3,4-tetrachlorobenzene, 1,2,3,5-tetrachlorobenzene and pentachlorobenzene was performed in reversed-phase system with octadecylsihca gel stationary phase and aqueous-methanohc mobile phase. Successful separation of ionic and polar compounds as water pollutants such as: benzenesulphonamide, benzenesulphonic acid, 1,3-benzenedisulphonic acid, 4-chlorobenzenesulphonic acid, 2,5-dichlorobenzenesulphonyl chloride, 2,3,4-trichlorobenzenesulphonyl chloride and 2,4,5-trichlorobenzenesulphonyl chloride requires addition of a salt (sodium sulphate) to the mobile phase.Addition of a buffer or of an ion-pair reagent to aqueous-methanohc mobile phases did not yield satisfactory improvement in separation selectivity nor of peak shape. Polar and ionic compounds, diphenylsulphone and chlorobenzenes, were separated and determined by a Iwo-step, reversed-phase LC method. On-line sample enrichment on an adsorption column packed either with octadecyl silica orwith aminopropyl silica connected to an LC column improved the limits of detection of the pollutants.

The evaluation and comparison of trigonal and linear tricationic ion-pairing reagents for the detection of anions in positive mode ESI-MS

“…A LOD of 200 pg for benzenedisulfonate by LC-UV was reported [19]. Using our method and TTC 1, the LOD for the same analyte is 8.75 pg using SIM detection and 500 fg using LTC 1 and SRM detection.…”

“…Common separation methods include ion chromatography [1,[12][13][14][15], ion pair chromatography [3,11,16], reverse-phase mode chromatography [17][18][19], and capillary electrophoresis (CE) [4,5,20]. To enhance the spectroscopic detection of anions that do not contain a UV chromophore, some CE and high-performance liquid chromatography (HPLC) methods utilize sample derivatization [21,22] or indirect UV or fluorescence detection methods [23][24][25].…”

Xenobiotics in the Urban Water Cycle

“…Besides, due to their poor lipophilicity, sulphonatd aromatic amines are considered to be less dangerous than their unsulphonated analogues (Jandera et al 2001;Alonso et al 2005). The fate of naphthalene sulphonates and their degradation products in the aquatic ecosystem as well as in biological treatment facilities is still not very clear since until now only limited attention has been paid towards their occurrence and degradability in treatment systems as well as in the natural environment (Jandera et al 2001;Song et al 2003).…”

Is the naphthalene sulfonate H-acid biodegradable in mixed microbial cultures under aerobic conditions?

“…The fate of naphthalene sulfonates in biological treatment systems is not very clear since until now only limited attention has been paid towards their occurrence and degradability (Jandera et al, 2001;Song et al, 2003). Depending upon their molecular structure and/or other physicochemical properties, naphthalene sulfonates may be degradable in biological treatment systems at very slow rates and upon acclimation (O'Neill et al, 1999).…”