HPLC determination of Vitamin D3 and its metabolite in human plasma with on-line sample cleanup

Brunetto, Marı́a del Rosario; Obando, María Antonieta; Gallignani, Máximo; Alarcon, O. M.; Nieto, Edgar; Salinas, Rafael Gil; Burguera, J.L.; Burguera, M.

doi:10.1016/j.talanta.2004.04.035

Cited by 30 publications

(11 citation statements)

References 31 publications

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“…In contrast to this the other HPLC methods reported the LOD ranged between 3 and 7.5 nmol/L while the LOQ ranged between 10 and 17.5 nmol/L [22][23][24][25][26].…”

Section: Sensitivitycontrasting

confidence: 81%

An Improved and Sensitive Method for Vitamin D3 Estimation by RPHPLC

Chawla¹,

Tripathi²

2015

Pharm Anal Acta

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“…In contrast to this the other HPLC methods reported the LOD ranged between 3 and 7.5 nmol/L while the LOQ ranged between 10 and 17.5 nmol/L [22][23][24][25][26].…”

Section: Sensitivitycontrasting

confidence: 81%

An Improved and Sensitive Method for Vitamin D3 Estimation by RPHPLC

Chawla¹,

Tripathi²

2015

Pharm Anal Acta

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“…Switching techniques with capillary columns have also been used for analysis of peptides in dialysate samples, but with electrochemical detection [19]. Column-switching techniques are also employed for conventional sized LC-MS systems [20], but such an approach would not be useful for this study since the samples were only available in relatively small amounts (450 lL) and it was expected that the total mass of analyte in the sample would be so small that (lC techniques would be required for analyte identification and quantification.…”

Section: Introductionmentioning

confidence: 99%

Determination of bradykinin and arg‐bradykinin in rat muscle tissue by microdialysis and capillary column‐switching liquid chromatography with mass spectrometric detection

Wilson¹,

Boix

Holm

et al. 2005

J of Separation Science

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Quantification of bradykinin peptides in limited amounts of rat muscle tissue dialysate has been performed using a packed capillary LC-ESI-TOF-MS method. The micro dialysate samples (450 microL) with added internal standard were loaded onto a 1 mm x 5 mm loading column packed with 5 microm Kromasil C18 particles by a carrier solution of 0.1% formic acid in ACN/water (5:95, v/v) at a flow rate of 250 microL/min for online preconcentration of the analytes. Back-flushed elution onto a 150 mm x 0.5 mm Zorbax C18 column packed with 5 microm particles was conducted using a linear solvent ACN/H2O gradient containing 0.1% formic acid. (Tyr8)-bradykinin was used as an internal standard and was added to the dialysis sample prior to injection. Baseline separation of bradykinin, arg-bradykinin and (tyr8)-bradykinin was achieved within 10 min. Positive ESI was performed in the m/z range of 200-1300. The method was validated in the range 0.2-1.0 ng/mL dialysate, yielding correlation coefficients of 0.995 and 0.990 for bradykinin and arg-bradykinin, respectively. The within-assay and between-assay precisions were between 4.3-9.6% and 6.2-10.6%, respectively. Both arg-bradykinin and bradykinin were detected in dialysate from rat muscle tissue, at concentrations of 0.1 and 0.4 ng/mL for bradykinin and arg-bradykinin, respectively, confirming the presence of arg-bradykinin in rat muscles.

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“…All measurements were run in duplicate and the mean values were calculated. Retinol, Vitamin D and Vitamin C were measured by HPLC (High-performance liquid chromatography), and folic acid and PTH were measured using an Immulite 2,000 device (Siemens, Germany) [ 8 9 10 11 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of seasonal changes on nutritional status and biochemical parameters in Turkish older adults

et al. 2018

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BACKGROUND/OBJECTIVESAvailable data suggest that seasonal changes may influence the nutritional status and overall health of elderly individuals. Therefore, this study was conducted to investigate the effects of seasonal changes and related factors on energy and nutrient intake of older adults.SUBJECTS/METHODSIndividuals aged 65 years or over were prospectively enrolled in this single-center study (male: 11, female: 20). Data were collected between May 2013 and February 2014 during winter, spring, summer and autumn. Food consumption and biochemical parameters were taken during each season to assess the seasonal nutrition status of the elderly. Upon analysis of biochemical parameters (retinol, vitamin D and vitamin C), an high-performance liquid chromatography device was utilized whereas an Immulite 2000 device was utilized during analysis of serum folic acid and parathyroid hormone.RESULTSFruit, fat, egg and bread consumption varied seasonally in males and females (P < 0.05). During winter, daily energy intake was found to be greater than in other seasons in males (557 kcal) and females (330 kcal) (P < 0.05). Additionally, carbohydrates, vegetable protein, n-3 fatty acid and sodium intake increased in winter, while the n-6/n-3 ratio increased in summer among males (P < 0.05). Dietary fiber and sodium intake in winter, vitamin C, iron and zinc intake in spring, and cholesterol, retinol, vitamin D and niacin intake in autumn were found to be higher in females when compared to other seasons (P < 0.05). Serum parathyroid hormone level was higher in winter, and vitamin D level was higher in autumn in both genders (P < 0.05). In males, blood folic acid level was higher in winter, while vitamin C level was higher in females, and there was no seasonal variation in retinol concentration (P < 0.05).CONCLUSIONFood consumption and biochemical parameters showed significant seasonal variations in older adults. It is not clear if nutrition plans in older adults will benefit from consideration of seasonal changes in eating habits.

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HPLC determination of Vitamin D3 and its metabolite in human plasma with on-line sample cleanup

Cited by 30 publications

References 31 publications

An Improved and Sensitive Method for Vitamin D3 Estimation by RPHPLC

An Improved and Sensitive Method for Vitamin D3 Estimation by RPHPLC

Determination of bradykinin and arg‐bradykinin in rat muscle tissue by microdialysis and capillary column‐switching liquid chromatography with mass spectrometric detection

Effect of seasonal changes on nutritional status and biochemical parameters in Turkish older adults

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