HPLC Evaluation of Phenolic Profile, Nutritive Content, and Antioxidant Capacity of Extracts Obtained fromPunica granatumFruit Peel

Abstract

This study revealed polyphenolic content, nutritive content, antioxidant activity, and phenolic profile of methanol and aqueous extracts of Punica granatum peel extract. For this, extracts were screened for possible antioxidant activities by free radical scavenging activity (DPPH), hydrogen peroxide scavenging activity and ferric-reducing antioxidant power (FRAP) assays. The total phenolics and flavonoid recovered by methanolic (MPE) and the water extract (AQPE) were ranged from 185 ± 12.45 to 298.00 ± 24.86 mg GAE (gallic acid equivalents)/gm and 23.05 ± 1.54 to 49.8 ± 2.14 quercetin (QE) mg/g, respectively. The EC50 of herbal extracts ranged from 100 µg/ml (0.38 quercetin equivalents), for AQPE, 168 µg/ml (0.80 quercetin equivalents), for MPE. The phenolic profile in the methanolic extracts was investigated by chromatographic (HPLC) method. About 5 different flavonoids, phenolic acids, and their derivatives including quercetin (1), rutin (2), gallic acid (3), ellagic acid (4), and punicalagin as a major ellagitannin (5) have been identified. Among both extracts, methanolic extract was the most effective. This report may be the first to show nutritive content and correlation analysis to suggest that phenols and flavonoids might contribute the high antioxidant activity of this fruit peel and establish it as a valuable natural antioxidant source applicable in the health food industry.

Chromatographic Determination of Phenolic Profile From Punica Granatum Fruit Peels

“…In this concern, the present finding was coincided with Hmid et al, (2013), who reported the presence of gallic, chlorogenic, caffeic, ferulic, ellagic acids, catechin, epicatechin, phloridzin, quercetin and rutin in P. granatum fruit juices 16 . Furthermore, Middha et al, (2013), revealed the presence of some major phenolic compounds such as gallic acid and ellagic acids in addition to punicalagin as a major ellagitannin in P. granatum fruit peel which were in agreements with the current studies 17 . On the other hand, the studies of Mansour et al, (2013), also examined the presence of quercetin and vanillic acid in the pomegranate fruit peel of Chinese and Tunisian variety although in the present study, quercetin was not found in P. granatum fruit peel 18 .…”

Analysis of toxic, antidiabetic and antioxidant potential of Bambusa balcooa Roxb. leaf extracts in alloxan-induced diabetic rats

“…The chromatographic peaks of the analytes were confirmed by comparing their retention time (Rt) and UV spectra with those of the reference standards. Integrated peak areas of the samples using the corresponding standard were used to quantify the compounds (Middha et al 2013). …”

Use of Phytoestrogens for the Treatment of Psychiatric Symptoms Associated with Menopause Transition

“…Interestingly, most of the health beneits of pomegranate are atributed to its high content of polyphenols, which represents the 26-30% of the total weight of the fruit [85]. The main polyphenols present in the pomegranate are ellagitannins, such as punicalagin (α and β), and lavonoids such as anthocyanidins, catechins, lavonols and isolavones [80,[86][87][88]. Ellagitannins are a type of hydrolyzable tannins with several hexahydroxydiphenoyl (HHDP) groups esteriied to sugar moieties.…”