HPLC method development for determination of doxycycline in human seminal fluid

Sunarić, Slavica; Denić, Marko; Bojanić, Zoran; Bojanić, Vladmila

doi:10.1016/j.jchromb.2013.08.035

Cited by 32 publications

(17 citation statements)

References 14 publications

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“…Therefore, the detection of Dox residues is important. In the past years, many analytical methods have been used to detect Dox, such as high-performance liquid chromatography, [7][8][9] electroluminescent molecularly imprinted polymer membranes, 10 spectrouorimetry, 11 electrochemistry, 12 immunosorbent assay, 13 liquid chromatography-ultraviolet, and liquid chromatography-mass spectrometry. 14 However, these methods require complicated sample pretreatment, expensive apparatus, and costly operation, limiting practical Dox detection in many elds.…”

Section: Introductionmentioning

confidence: 99%

A novel fluorescent sensor based on sulfur and nitrogen co-doped carbon dots with excellent stability for selective detection of doxycycline in raw milk

Song

Guo

et al. 2017

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

A novel fluorescent sensor based on sulfur and nitrogen co-doped carbon dots with excellent stability for selective detection of doxycycline in raw milk

Song

Guo

et al. 2017

RSC Adv.

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“…After centrifugation under the same conditions, the obtained supernatant (approx 60µl) was injected into the chromatographic system for analysis. 10 All these solutions were stable for seven days when stored at room temperature (20-25 ᵒC) and were used within one week. The bulk spiked CC samples were stored at −20 ∘ C and brought to room temperature before use.…”

Section: Preparation Of Calibration Curve (Cc)mentioning

confidence: 99%

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2017

IJPSR

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A novel method of estimation and validation of Atorvastatin by Reverse Phase-High Performance Liquid Chromatography coupled with Ultra-violet detection was developed which had high potential in determining drug concentration in rat plasma samples during preclinical studies. Atorvastatin being >99% protein bound, exhibits great challenge in getting extracted from plasma samples. Hence, by treating them with strong protein precipitating agents, that is, initially with 10% w/v perchloric acid and further treating its supernatant with mixture of 2M potassium carbonate and 3M of potassium hydroxide, drug extraction was facilitated. Diclofanac sodium was the selected internal standard. Process of elution was conducted using Phenomenex C 18 column and mobile phase comprising of a mixture of methanol: water in 70:30 ratio adjusted to pH 5.5. This precise method was linear between a range of 10 to 1000 ng/ml with limit of detection and quantification as 10ng/ml and 15ng/ml respectively. 2-factor 3-level face centred Central Composite Design was employed using Design Expert Software ver. 8.0.0 to examine effect of independent chromatographic factors like pH of the mobile and methanol: water ratio on the dependent factors like retention time, theoretical plates and tailing factor. The ANOVA studies proved that the model employed for this study was significant. Further, to improve applicability, marketed drug formulation was spiked in rat plasma and developed method was applied for drug detection.

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“…Tetracyclines were determined in biological fluids (blood serum and plasma [75,[125][126][127][128], urine [75,121,125,127,129], seminal fluid [130], and saliva [131]) after the precipitation of proteins with trichlo roacetic [75] or perchloric [125,130] acids, acetoni trile [131], or buffer solutions [39,75,121,[126][127][128]. The samples were additionally purified by SPE [75,125,129] with the use of MP1 Varian [75] and Oasis HLB [129] cartridges.…”

Section: Sample Preparationmentioning

confidence: 99%

Methods for the separation, preconcentration, and determination of tetracycline antibiotics

2015

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The data published in the last five years concerning the determination of tetracycline antibiotics in different samples are generalized. Sample preparation procedures for real samples, including liquid extrac tion from solid matrices, pressurized liquid extraction, matrix solid phase dispersion, QuEChERS method, and solid phase extraction, are compared. The following important determination techniques are discussed: chromatographic, electrophoretic, spectroscopic, and electrochemical. The determination of the above anti biotics in food products, feed, environmental samples, pharmaceuticals, and biological fluids is exemplified.

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HPLC method development for determination of doxycycline in human seminal fluid

Cited by 32 publications

References 14 publications

A novel fluorescent sensor based on sulfur and nitrogen co-doped carbon dots with excellent stability for selective detection of doxycycline in raw milk

A novel fluorescent sensor based on sulfur and nitrogen co-doped carbon dots with excellent stability for selective detection of doxycycline in raw milk

Untitled

Methods for the separation, preconcentration, and determination of tetracycline antibiotics

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