HPLC method for simultaneous determination of cefprozil diastereomers in human plasma

Park, Tae Hwan; Kim, Jin‐Ki; Jee, Jun‐Pil; Park, Jeong-Sook; Kim, Chong‐Kook

doi:10.1016/j.jpba.2004.06.001

Cited by 18 publications

(17 citation statements)

References 12 publications

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“…The bioassay method has been employed, but it cannot distinguish cis-and trans-isomers in biological matrices (Nye et al, 1990). The HPLC method has been successfully applied to the simultaneous determination of cefprozil isomers in human plasma (Shyu et al, 1991b;Park et al, 2004). However, there are still some limitations such as long analysis time, complex mobile phase composition and endogenous peak interference, and so it cannot meet the needs of clinical pharmacokinetic and bioequivalence studies.…”

Section: Introductionmentioning

confidence: 99%

An LC‐MS/MS method for simultaneous determination of cefprozil diastereomers in human plasma and its application for the bioequivalence study of two cefprozil tablets in healthy Chinese volunteers

Liu

Zhang

et al. 2015

Biomedical Chromatography

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A rapid and sensitive liquid chromatography-tandem mass spectrometric method was developed for the first time and validated for the determination of cefprozil diastereomers in human plasma. The plasma samples were prepared by protein precipitation using acetonitrile. Detection was performed using an electronic spray ion source in the negative ion mode, operating in the multiple reaction monitoring of the transitions m/z 388.0 to m/z 205.0 for cefprozil diastereomers and m/z 346.1 to m/z 268.1 for cephalexin (the internal standard). The calibration curves of cis-cefprozil and trans-cefprozil were linear in the ranges 0.125-16.0 µg/mL and 0.0403-1.72 µg/mL, respectively. The lower limits of quantification of cis- and trans-cefprozil were 0.125 and 0.0403 µg/mL in human plasma, respectively. The intra- and inter-day precisions of cis- and trans-cefprozil were all <9.7%, and the accuracy ranged from 99.2 to 104.7% and from 100.6 to 102.2%, respectively. The validated method was successfully applied to a bioequivalence study of two cefprozil formulations in 24 healthy Chinese volunteers. The two cefprozil tablets were bioequivalent by measurement of cis-, trans- and total cefprozil. We suggest that the bioequivalence of cefprozil formulations can be evaluated only using cis-cefprozil as the analyte in future studies.

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Section: Introductionmentioning

confidence: 99%

An LC‐MS/MS method for simultaneous determination of cefprozil diastereomers in human plasma and its application for the bioequivalence study of two cefprozil tablets in healthy Chinese volunteers

Liu

Zhang

et al. 2015

Biomedical Chromatography

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“…The most methods of analysis of the drug in bulk form or in its pharmaceutical formulations rely on the use of high-performance liquid chromatography (HPLC) methods. Few techniques have been reported to determine cefprozil either in pure form, pharmaceutical preparations or biological fluids; including colorimetry [3][4][5], UV spectrophotometry [6] and HPLC [7][8][9]. Abdel Sattar et al [3] described a spectrophotometric method for the determination of cefprozil.…”

Section: Introductionmentioning

confidence: 99%

Flow-injection chemiluminescent determination of cefprozil using Tris (2,2′-bipyridyl) ruthenium (II)-permanganate system

Alarfaj

El-Razeq

2006

Journal of Pharmaceutical and Biomedical Analysis

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“…Earlier publications have reported high-performance liquid chromatography (HPLC) [5] and high performance thin-layer chromatography (HPTLC) [6] methods for quantification of Cefprozil in human plasma and pharmaceutical dosage form. However, these methods involve arduous sample preparation Chronicles of Young Scientists  159  and long chromatographic run times for biological samples.…”

Section: Introductionmentioning

confidence: 99%

Application of UV spectrophotometric method to study stress degradation behavior of cefprozil

Jadhav¹,

Dhamecha²,

Asnani³

et al. 2013

Chron Young Sci

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Background: Cefprozil is a bactericidal drug that is used in the treatment of susceptible infections including upper and lower respiratory tract infections and skin and soft tissue infections. Materials and Methods: The objective of this research work was to develop and validate new, simple ultraviolet (UV) spectrophotometric method of Cefprozil in bulk and pharmaceutical dosage form and its application to study its stress degradation behavior. Results: The absorbance maxima peak was found at 280 nm, and linearity was observed in the concentration range of 2-10 µg/ml. The method was validated and found to be precise. Accuracy (percent recovery) for Cefprozil was found to be 99.117±1.005. Conclusion: A new method for estimation of Cefprozil by UV spectrophometry was developed and validated and Cefprozil was found to undergo degradation in all stress conditions.

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HPLC method for simultaneous determination of cefprozil diastereomers in human plasma

Cited by 18 publications

References 12 publications

An LC‐MS/MS method for simultaneous determination of cefprozil diastereomers in human plasma and its application for the bioequivalence study of two cefprozil tablets in healthy Chinese volunteers

An LC‐MS/MS method for simultaneous determination of cefprozil diastereomers in human plasma and its application for the bioequivalence study of two cefprozil tablets in healthy Chinese volunteers

Flow-injection chemiluminescent determination of cefprozil using Tris (2,2′-bipyridyl) ruthenium (II)-permanganate system

Application of UV spectrophotometric method to study stress degradation behavior of cefprozil

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