HPLC method for the analysis of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L.

Kartal, Murat; Altun, Mehmet; Kurucu, Semra

doi:10.1016/s0731-7085(02)00568-x

Cited by 158 publications

(117 citation statements)

References 8 publications

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“…The aqueous acid layer was then made alkaline (pH 10) using NH 4 OH and extracted four times with 50 ml chloroform. The resulting chloroform layer was combined and evaporated to dryness, and the residues were dissolved in 3 ml methanol (Kartal et al 2003). The extracts obtained above were then passed through a 0.45-μm filter and 10 μl of the filtrate extract was directly injected into the HPTLC column made of aluminum sheets of silica gel 60 F254 (Merck).…”

Section: Extraction and Measurement Of ß-Carboline Alkaloidsmentioning

confidence: 99%

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Sharifi

Sattari

Zebarjadi

et al. 2013

Physiol Mol Biol Plants

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We have developed an efficient transformation system for Tribulus terrestris L., an important medicinal plant, using Agrobacterium rhizogenes strains AR15834 and GMI9534 to generate hairy roots. Hairy roots were formed directly from the cut edges of leaf explants 10-14 days after inoculation with the Agrobacterium with highest frequency transformation being 49 %, which was achieved using Agrobacterium rhizogenes AR15834 on hormone-free MS medium after 28 days inoculation. PCR analysis showed that rolB genes of Ri plasmid of A. rhizogenes were integrated and expressed into the genome of transformed hairy roots. Isolated transgenic hairy roots grew rapidly on MS medium supplemented with indole-3-butyric acid. They showed characteristics of transformed roots such as fast growth and high lateral branching in comparison with untransformed roots. Isolated control and transgenic hairy roots grown in liquid medium containing IBA were analyzed to detect ß-carboline alkaloids by High Performance Thin Layer Chromatograghy (HPTLC).Harmine content was estimated to be 1.7 μg g −1 of the dried weight of transgenic hairy root cultures at the end of 50 days of culturing. The transformed roots induced by AR15834 strain, spontaneously, dedifferentiated as callus on MS medium without hormone. Optimum callus induction and shoot regeneration of transformed roots in vitro was achieved on MS medium containing 0.4 mg L −1 naphthaleneacetic acid and 2 mg L −1 6-benzylaminopurine (BAP) after 50 days. The main objective of this investigation was to establish hairy roots in this plant by using A. rhizogenes to synthesize secondary products at levels comparable to the wild-type roots.

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Section: Extraction and Measurement Of ß-Carboline Alkaloidsmentioning

confidence: 99%

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Sharifi

Sattari

Zebarjadi

et al. 2013

Physiol Mol Biol Plants

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“…Peganum harmala is a rich source of b-carboline and quinazoline alkaloids (Kartal et al, 2003). The possible use of P. harmala in modern phyto-indole entheogen preparations is correlated to its b-carboline content: harmine, harmaline, and tetrahydroharmine (THH), collectively known as harmala alkaloids, which are mostly found in the seeds and roots.…”

Section: Abstract: Peganum Harmala Topical Bioassay Aphid Speciesmentioning

confidence: 99%

Toxic and Repellent effecto of Harmal (Peganum harmala L.) Acetonic Extract on Several Aphids and Tribolium castaneum (Herbst)

Salari

Ahmadi

Dehyaghobi

et al. 2012

Chilean J. Agric. Res.

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To reduce the dependence on the sometimes unwise use of synthetic pesticides in fruit and vegetable plantations, the toxicity and repellence of Peganum harmala L. (Zygophyllaceae) acetonic seed extract was assayed against several insect pests. For contact toxicity, 3-to 4-d-old individuals of Aphis fabae Scopoli, A. gossypii Glover, A. nerii Boyer de Fonscolombe, and Myzus persicae (Sulzer) were included, as well as 1-to 7-d-old adult Tribolium castaneum (Herbst). Repellent effect experiments were conducted on adult, 1-to 2-and 3-to 4-d old M. persicae individuals. At 60 mg mL -1 , the topical bioassay mortality percentage was significantly higher in A. gossypii than in A. fabae and A. nerii after 12-72 h. Mortality of the treatments on M. persicae was 87.1% and 90.0% after 24 and 48 h, respectively, and significantly higher than A. fabae and A. nerii during this period. At 60 mg mL -1 , the mortality of T. castaneum was much lower than that of the aphid species. The highest repellent index (over 72%) was observed on 1-to 2-d-old M. persicae individuals.

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“…We used the method of kartal with some modifications [21]. Ten grams of dried and powdered seeds of Peganum harmala were macerated four times with 50ml 70% methanol at 50˚C in a water bath for 1h.…”

Section:  Extraction Processmentioning

confidence: 99%

A framework for harmla alkaloid extraction process development using fuzzy-rough sets feature selection and J48 classification

Badria¹,

Habib²,

Shoaip³

et al. 2017

IJACR

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HPLC method for the analysis of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L.

Cited by 158 publications

References 8 publications

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Toxic and Repellent effecto of Harmal (Peganum harmala L.) Acetonic Extract on Several Aphids and Tribolium castaneum (Herbst)

A framework for harmla alkaloid extraction process development using fuzzy-rough sets feature selection and J48 classification

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