HPLC method for the determination of ecdysterone in extractive solution from Pfaffia glomerata

Zimmer, Aline Rigon; Bruxel, Fernanda; Bassani, Valquíria Linck; Gosmann, Grace

doi:10.1016/j.jpba.2005.07.016

Cited by 32 publications

(26 citation statements)

References 10 publications

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“…Quantification of β-ecdysone was performed using the HPLC and a method with methanol and water, a solvent lower cost, different of Zimmer et al (2006) which used acetonitrile and water in the mobile phase.…”

Section: Validationmentioning

confidence: 99%

Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC

Serra¹,

Felipe²,

Cortez³

2012

Rev. bras. farmacogn.

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Pfaffi a glomerata (Spreng.) Pedersen, Amaranthaceae, is widely distributed in Brazil. Roots are considered as the world's greatest supplier and β-ecdysone is the most important compound extracted from roots of Pfaffi a glomerata. So, the aim this study was analyze the presence of β-ecdysone in the infl orescences and stems and compared with the content from roots of Pfaffi a glomerata and determine the best extractive method of β-ecdysone this plant. The crude extracts were obtained by Soxhlet method, refl ux, maceration, percolation and turbolyse. Compound extracts were quantifi ed by High Performance Liquid Chromatography (HPLC). The analysis were carried out a Phenomenex Column C18, 5 µm, 250x4,6mm, maintened at 30 o C, gradient system using as mobile phase a mixture of methanol and water, fl ow rate 1,0 mL and detection at 245 nm. Results showed Soxhlet method with ethanol:water (90:10 v/v) presented the higher concentration of β-ecdysone in P. glomerata and infl orescences showed higher amount of this active substance (3,06%), compared with stems (2,37%) and roots (1,63%), showing that the infl orescences and plant stems may also be used as a rich source of β-ecdysone.

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Section: Validationmentioning

confidence: 99%

Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC

Serra¹,

Felipe²,

Cortez³

2012

Rev. bras. farmacogn.

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“…glomerata é a espécie de maior importância medicinal e comercial, cujas raízes são utilizadas como matéria prima para a produção de fitoterápicos e suplementos alimentares (Figueiredo et al, 2004;Zimmer et al, 2006). Estudos farmacológicos demonstraram que extratos de raízes de P. glomerata favoreceram a aprendizagem e a memória (Marques et al, 2004), apresentaram efeitos antiinflamatórios, analgésicos (Neto et al, 2005) e antioxidantes (Daniel et al, 2005).…”

Section: Introductionunclassified

Benzilaminopurina (BAP) e thidiazuron (TDZ) na propagação in vitro de Pfaffia glomerata (Spreng.) Pedersen

Flores

Nicoloso

Maldaner

et al. 2009

Rev. bras. plantas med.

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RESUMO:Pfaffia glomerata (Spreng.) Pedersen, conhecida como ginseng brasileiro, é muito utilizada pela medicina popular devido suas propriedades fitoterápicas. Este trabalho teve como objetivo avaliar o efeito de concentrações de BAP e de TDZ na propagação in vitro de dois acessos (BRA e JB-UFSM) desta espécie. Segmentos nodais, provenientes de plantas assépticas, foram cultivados em meio Murashige e Skoog (MS) suplementado com 0, 1 e 5 M de BAP ou TDZ. Após 30 dias, as plantas foram transferidas para meio MS não suplementado com citocinina. Observou-se que a organogênese in vitro de P. glomerata é genótipo-dependente. O acesso BRA mostrou um maior potencial para a propagação in vitro em relação ao JB-UFSM. O cultivo dos segmentos nodais do acesso BRA em meio com 1 M de TDZ, seguido pelo subcultivo dos brotos para meio isento de citocininas mostrou ser um método viável para a propagação in vitro devido à alta taxa de multiplicação e o bom desenvolvimento das plantas. No acesso JB-UFSM, o cultivo dos segmentos nodais em meio não acrescido de citocinina mostrou ser o método mais adequado para a produção de um grande número de plantas com alta qualidade.Palavras chave: Amaranthaceae, ginseng brasileiro, cultivo in vitro, citocininas ABSTRACT: Benzylaminopurine (BAP) and thidiazuron (TDZ) on in vitro propagation ofPfaffia glomerata (Spreng.) Pedersen. The plant Pfaffia glomerata (Spreng.) Pedersen, known as Brazilian ginseng, is extensively used in folk medicine due to its phytotherapic characteristics. This work aimed to evaluate the effect of BAP and TDZ concentrations on the in vitro propagation of two sources (BRA and JB-UFSM) of this species. Nodal segments originated from aseptically grown plants were cultured on Murashige and Skoog (MS) medium supplemented with 0, 1 and 5 M BAP or TDZ. After 30 days, the plants were transferred to MS medium without cytokinin. The in vitro organogenesis of P. glomerata is genotype-dependent. The source BRA had greater potential for in vitro propagation than JB-UFSM. The culture of BRA nodal segments on medium with 1 M TDZ, followed by subcultivation of sprouts on cytokinin-free medium, showed to be a viable method for in vitro propagation due to high multiplication rate and good plant development. In the source JB-UFSM, the culture of nodal segments on medium without cytokinin was the best method to obtain a large number of high-quality plants.

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“…Twentycubic centimeterof the ethyl acetate solution produced by PLE was dispersed into 80 cm 3 of an aqueous solution withOSA-modified starch surfactant (6 g/dm 3 ) by the aid of a h igh speed-stirring mixer(IKA® magic LA B ® , Staufen, Germany) with an engine power of 900 Watt processed during 4 min at 26.000 rp m. The mixer was cooled by ethylene glycol that circulates through a jacket, which allo ws to remove the heat generated by the equipment and to operate at temperatures lower than 298 K to avoid ethyl acetate evaporation.…”

Section: Supercritical Flui D Extracti On Of Emulsions (Sfee) Processmentioning

confidence: 99%

“…Due to the similar mo rphology of its roots to those of ginseng, they are popularly known as "Brazilian ginseng". Around 90 species of Pfaffia are known in Central and South America [3]. In Brazil, 27 species have been described, being Pfaffia glomerata the most important specie.…”

Section: Introductionmentioning

confidence: 99%

Pressurized Organic Solvent Extraction with On-line Particle Formation by Supercritical Anti Solvent Processes

Santos¹,

Barbosa²,

Broccolo³

et al. 2012

fph

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In this work, anovel on-line process for pressurised hot organic solvent extraction of antio xidants from plantsas well as precip itation of the extract with or without a carrier material in one step was developed. This process has been called OEPO,Organic solvent Ext raction and On-line particle formation. With this process, different products with a very low residual organic solvent concentration (< 50 ppm) can be obtained by the use of supercritical CO 2 as anti solvent for solvent elimination.OEPO process consists of hyphenated Pressurized Liquid Extraction (PLE)-Supercritical Anti So lvent (SAS) precipitation, PLE-SAS co-p recipitat ion and PLE-Supercritical Fluid Ext raction of Emulsions (SFEE). OEPO process was successfully developed using Brazilian g inseng roots (Pfa ffiaglomerata)as a model case using ethyl acetate as extracting solvent. Results were co mpared, in terms of antio xidant activity o r mo rphology, with the ones obtained by each process separately.In addition, an optimizat ion study for antio xidants recovery was performed using ethyl acetate as extracting solvent during PLE process. Optimu m PLE extracts were produced under moderate extraction temperature (373 K) and high static extraction t ime (15 min). Under this condition an ext raction yield o f 1% (d ry basis, d.b.) and an antioxidant activity of 53% are obtained, wh ich was appro ximately 14% higherthan that observed after PLE-SAS p recipitation and after SAS precipitation performed in two steps (step one -PLE ext raction; step two -SAS precipitation by the use of the ext ract solution produced by step one stored).Similar behavior (hyphenated process producing similar p roducts than the two step process done separately) was observed for PLE-SAS co-precip itation and PLE-SFEE indicating that the OEPO process developed in this work can be considered as a suitable and p ro mising process to obtain, in only one step, different products (precipitated extract, co-precipitated extract or encapsulated extract in suspension), direct ly fro m plant materials.

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HPLC method for the determination of ecdysterone in extractive solution from Pfaffia glomerata

Cited by 32 publications

References 10 publications

Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC

Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC

Benzilaminopurina (BAP) e thidiazuron (TDZ) na propagação in vitro de Pfaffia glomerata (Spreng.) Pedersen

Pressurized Organic Solvent Extraction with On-line Particle Formation by Supercritical Anti Solvent Processes

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HPLC method for the determination of ecdysterone in extractive solution from Pfaffia glomerata

Cited by 32 publications

References 10 publications

Quantification of &#946;-ecdysone in differents parts of Pfaffia glomerata by HPLC

Quantification of &#946;-ecdysone in differents parts of Pfaffia glomerata by HPLC

Benzilaminopurina (BAP) e thidiazuron (TDZ) na propagação in vitro de Pfaffia glomerata (Spreng.) Pedersen

Pressurized Organic Solvent Extraction with On-line Particle Formation by Supercritical Anti Solvent Processes

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Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC

Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC