HPLC method validation for measurement of sulforaphane level in broccoli by‐products

Abstract

A simple and specific analytical method was developed and tested for the determination of sulforaphane in broccoli by-products. The method includes the optimization of the conversion of glucoraphanin to sulforaphane, followed by purification of extracts using solid-phase extraction and high-performance liquid chromatography (HPLC) analysis. The response surface methodology was used to find optimum conditions for the preparation and purification procedure. Chromatographic conditions for reversed-phase HPLC with UV photodiode array detection were as follows: column, Exil ODS C(18), 25 x 0.46 cm, 5 microm; column temperature, 36 degrees C; mobile phase, a 30 : 70 (v/v) mixture of acetonitrile:water; flow rate, 0.6 mL/min. The detection wavelength was UV 202 nm. Under these conditions, excellent linearity was obtained (r(2) = 1), and the overall recovery was 97.5 and 98.1% for fresh florets and lyophilized florets, respectively. The precision results showed that the relative standard deviation of the repeatability for florets fresh and lyophilized was 3.0 and 4.0%, respectively. Sulforaphane contents were determined in the edible portion of fresh broccoli, and broccoli crop remains.

Optimisation of enzymatic production of sulforaphane in broccoli sprouts and their total antioxidant activity at different growth and storage days

“…In the present study, 2 h was to be the best hydrolysis time for sulforaphane production; either shorter or longer hydrolysis times affected myrosinase activity. This result was in line with the work of Campas-Baypoli et al (2010). Choi et al (2004) reported considerable differences in reaction time and observed that the reaction rate appears to depend on the structure of ITCs.…”

Modification of glucosinolates in turnip greens (Brassica rapasubsp.rapaL.) subjected to culinary heat processes

“…Phytochemicals are found in all plant parts; the seeds, roots, and the inflorescences are the parts that have higher concentrations of glucosinolates, followed by the leaves and finally the stems (Campas-Baypoli, Sánchez-Machado, Bueno-Solano, Ramírez-Wong, & López-Cervantes, 2009). Other factors that may potentially influence and alter the content of glucosinolates are cultural practices, storage conditions, and preparation of food (Yábar, Pedreschi, Chirinos, & Campos, 2011).…”

Development and validation of a LC–MS/MS method to determine sulforaphane in honey

“…SFN has been widely determined in other matrices such as Brassica vegetables (Ares et al, 2014;Azizi, Amiri-Besheli, & SharifiMeher, 2011;Campas-Baypoli, Sánchez-Machado, Bueno-Solano, Ramírez-Wong, & López-Cervantes, 2010;Ghawi, Methven, & Niranjan, 2013;Guo, Guo, Wang, Zhuang, & Gu, 2013;Han & Row, 2011;Liang, Yuan, & Liu, 2013;Matusheski et al, 2001;Moon, Kim, Ahn, & Shibamoto, 2010;Nakagawa et al, 2006;Shen, Su, Wang, Du, & Wang, 2010;Sivakumar, Alboni, & Bacchetta, 2007) and biological ones (Agrawal et al, 2006;Dominguez-Perles et al, 2014;Hauder et al, 2011;Wang, Lin, Shen Khor, Nomeir, & Kong, 2011). Extraction of SFN from plant matrices was usually done by solvent extraction with an organic solvent such as dichloromethane (DCM) (Azizi et al, 2011;Campas-Baypoli et al, 2010;Ghawi et al, 2013;Guo et al, 2013;Han & Row, 2011;Matusheski et al, 2001;Nakagawa et al, 2006;Sivakumar et al, 2007), chloroform (Moon et al, 2010) or methyl t-butyl ether (MTBE) (Ares et al, 2014), and in many of the above-mentioned studies (Ares et al, 2014;Azizi et al, 2011;Campas-Baypoli et al, 2010;Guo et al, 2013;Matusheski et al, 2001;Sivakumar et al, 2007) solid phase extraction (SPE) with silica based cartridges was also performed to purify the plant extracts.…”