HPLC-MS Analysis of Ethanol Extract of Corydalis yanhusuo and Simultaneous Determination of Eight Protoberberine Quaternary Alkaloids by HPLC-DAD

Abstract

An accurate and simple analysis method by high-performance liquid chromatography (HPLC)-electrospray tandem mass spectrometry (ESI-MS-MS) for the ethanol extract of Corydalis yanhusuo was established in this study. In this method, a C(18) column was used, and the mobile phase was water (0.2% acetic acid, 0.1% triethylamine, v/v)-acetonitrile (24:76, v/v). Under the optimal analysis conditions established in this study, eight protoberberine quaternary alkaloids in the ethanol extract of Corydalis yanhusuo were well separated and identified, respectively. Eight regression equations showed a good linear relationship between the peak area of each marker and concentration (r = 0.9990-0.9995). The various other aspects of analysis (i.e., peak purity, precision, recovery, and repeatability) were also validated. For the eight components, the recoveries were found to be 98.9%, 99.2%, 105.0%, 97.7%, 101.4%, 98.6%, 98.5%, and 98.7%, respectively. The limits of detection were 14.6, 42.5, 22.4, 17.9, 25.4, 10.0, 18.7, 3.9 ng/mL, respectively, and the limits of quantitation were 48.6, 141.7, 74.6, 59.6, 84.8, 33.2, 62.3, and 12.9 ng/mL, respectively. The developed method is useful for the simultaneous determination of these alkaloids in a large number of ethanol extracts of Corydalis yanhusuo.

Influence of Rhizophora apiculata Blume extracts on α-glucosidase: Enzyme kinetics and molecular docking studies

“…After, that the modern analytical techniques have facilitated the isolation of alkaloids from plants is making it very simple. The use of 0.2% acetic acid and methanol as a solvent system reduces the ionization, more symmetrical peak and provides compelling evidence for alkaloids in HPLC-MS. Cheng et al (2010) also observed the better efficiency, using acetonitrile instead of methanol. However, mixtures consisting only of water and acetonitrile were not sufficient to separate completely the components in the ethanol extract, and some peaks overlapped.…”

Determination of armepavine in mouse blood by UPLC‐MS/MS and its application to pharmacokinetic study

“…Because alkaloids are generally not volatile, few of them can be analyzed by GC-MS. LC-MS and LC-MS/MS are often used for the analysis of alkaloids (Cheng, Shi, Zhen, Sun, & Jin, 2010;Gardner & Pfister, 2009;Liao, Chang, Yin, Wang, & Meng, 2014). Chromatography-mass *These authors contributed equally to this work.…”

“…Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS/MS) are the most commonly developed analytical methods to determine alkaloids. Because alkaloids are generally not volatile, few of them can be analyzed by GC-MS. LC-MS and LC-MS/MS are often used for the analysis of alkaloids (Cheng, Shi, Zhen, Sun, & Jin, 2010;Gardner & Pfister, 2009;Liao, Chang, Yin, Wang, & Meng, 2014).…”

Simultaneous determination of eleven alkaloids in Corydalis decumbens by HPLC with diode-array detection

“…Several analytical methods have been developed and used for the detection of analogous alkaloids in Corydalis yanhusuo, a plant from the same genus as that of C. decumbens, such as high-performance liquid chromatography (HPLC) [17][18][19][20], liquid chromatography-mass spectrometry (LC-MS) [21][22][23], capillary electrophoresis (CE) [24,25], and high-speed countercurrent chromatography (HSCCC) [26]. However, only a few quantificational studies of C. decumbens alkaloids have been published.…”